DUOX1 Antibody

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Western Blot: DUOX1 Antibody [NBP2-16232] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DUOX1 antibody [N1N3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG ...read more
Immunohistochemistry-Paraffin: DUOX1 Antibody [NBP2-16232] - Immunohistochemical analysis of paraffin-embedded gastric ca, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity Hu, Po, BvSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.95 mg/ml

Order Details

DUOX1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human DUOX1. The exact sequence is proprietary.
Localization
Apical cell membrane
Predicted Species
Porcine (90%), Bovine (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DUOX1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
177 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Mouse (89%), Canine (86%), Rat (87%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.95 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DUOX1 Antibody

  • dual oxidase 1
  • DUOX
  • EC 1.11.1.-
  • EC 1.6.3.1
  • Large NOX 1
  • LNOX1flavoprotein NADPH oxidase
  • Long NOX 1
  • MGC138840
  • NADPH thyroid oxidase 1
  • nicotinamide adenine dinucleotide phosphate oxidase
  • NOXEF1
  • THOX1MGC138841
  • Thyroid oxidase 1

Background

The protein encoded by this gene is a glycoprotein and a member of the NADPH oxidase family. The synthesis of thyroid hormone is catalyzed by a protein complex located at the apical membrane of thyroid follicular cells. This complex contains an iodide transporter, thyroperoxidase, and a peroxide generating system that includes this encoded protein and DUOX2. This protein is known as dual oxidase because it has both a peroxidase homology domain and a gp91phox domain. Two alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol DUOX1
Uniprot