Dtk/TYRO3 Antibody [Unconjugated]

Western blot shows lysates of human brain (cerebellum) tissue, U‑87 MG human glioblastoma/astrocytoma cell line, and IMR‑32 human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Tyro3/Dtk Antigen Affinity-purified Polyclonal Antibody (Catalog # AF859) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Tyro3/Dtk at approximately 97 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Dtk/TYRO3 Antibody [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Tyro3/Dtk
  Ala41-Ser428
  Accession # Q06418
• Specificity: Detects human Tyro3/Dtk in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse (rm) Dtk is observed and less than 1% cross-reactivity with recombinant human Axl, rmAxl, and rmMer is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TYRO3
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
• Control:

  Dtk/TYRO3 Knockout HeLa Cell Lysate

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Dtk/TYRO3 Antibody [Unconjugated]

• Brt
• BYK
• Dtk
• EC 2.7.10
• EC 2.7.10.1
• FLJ16467
• Rse
• Sky
• Tif
• TYRO3 protein tyrosine kinase
• Tyro3
• Tyrosine-protein kinase byk
• Tyrosine-protein kinase DTK
• tyrosine-protein kinase receptor TYRO3
• Tyrosine-protein kinase RSE
• Tyrosine-protein kinase SKY

Background

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a new receptor tyrosine kinase subfamily. The extracellular domain of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. All three receptors bind the vitamin K-dependent protein growth-arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. The binding affinities for Gas6 is in the order of Axl > Dtk > Mer. Gas6 binding induces tyrosine phosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis. Dtk is widely expressed during embryonic development. In adults, Dtk is predominantly expressed in neurons in restricted regions of the brain.

1. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
2. Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Dtk/TYRO3 Antibody (AF859)(3)

Publications using AF859

• A Kumar, J Jovel, J Lopez-Oroz, D Limonta, AM Airo, S Hou, I Stryapunin, C Fibke, RB Moore, TC Hobman Human Sertoli cells support high levels of Zika virus replication and persistence Sci Rep, 2018;8(1):5477. 2018 [PMID: 29615760] (Neutralization, Human)
• C Dantas-Bar, T Lesluyes, FL Loarer, F Chibon, I Treilleux, JM Coindre, P Meeus, M Brahmi, O Bally, I Ray-Coquar, MP Sunyach, AL Cesne, O Mir, S Bonvalot, M Toulmonde, A Italiano, P Saintigny, M Jean-Denis, F Ducimetier, D Ranchere, H El Sayadi, L Alberti, JY Blay Expression and role of TYRO3 and AXL as potential therapeutical targets in leiomyosarcoma Br. J. Cancer, 2017;117(12):1787-1797. 2017 [PMID: 29024938] (Flow Cytometry, Human)
• KE Happonen, S Tran, M Mörgelin, R Prince, S Calzavarin, A Angelillo-, B Dahlbäck The Gas6-Axl Protein Interaction Mediates Endothelial Uptake of Platelet Microparticles J. Biol. Chem., 2016;291(20):10586-601. 2016 [PMID: 27006397] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: TYRO3
• Uniprot:
  • Q06418()