Dtk/TYRO3 Antibody (96201) [Phycoerythrin]

K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human Dtk PE-conjugated Monoclonal Antibody (Catalog # FAB859P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 96201
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

Dtk/TYRO3 Antibody (96201) [Phycoerythrin] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Dtk
  Ala41-Ser428 (predicted)
  Accession # Q06418
• Specificity: Detects human Dtk in ELISAs and Western blots. In ELISAs and Western blots, no cross-reactivity or interference with recombinant mouse Dtk, recombinant human (rh) Axl, or rhMer is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TYRO3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Dtk/TYRO3 Antibody (96201) [Phycoerythrin]

• Brt
• BYK
• Dtk
• EC 2.7.10
• EC 2.7.10.1
• FLJ16467
• Rse
• Sky
• Tif
• TYRO3 protein tyrosine kinase
• Tyro3
• Tyrosine-protein kinase byk
• Tyrosine-protein kinase DTK
• tyrosine-protein kinase receptor TYRO3
• Tyrosine-protein kinase RSE
• Tyrosine-protein kinase SKY

Background

Dtk (also known as Sky, Tyro3, Rse, Brt), Axl (also known as Ufo, Ark) and Mer (human and mouse homologues of chicken c-Eyk) constitute a receptor tyrosine kinase subfamily. The extracellular domain of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. All three receptors bind the vitamin K-dependent protein growth-arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor Protein S. The binding affinities for Gas6 are in the order of Axl > Dtk > Mer. Gas6 binding induces tyrosine phosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis. Dtk is widely expressed during embryonic development. In adults, Dtk is predominantly expressed in neurons in restricted regions of the brain.

1. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
2. Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Dtk/TYRO3 Antibody (FAB859P)(9)

Publications using FAB859P

• JS Mytych, Z Pan, C Lopez-Davi, N Redinger, C Lawrence, J Ziegler, N Popescu, JA James, AD Farris Peptidoglycan from Bacillus anthracis Inhibits Human Macrophage Efferocytosis in Part by Reducing Cell Surface Expression of MERTK and TIM-3 bioRxiv : the preprint server for biology, 2023-05-10;0(0):. 2023-05-10 [PMID: 37066181] (Flow Cytometry, Human)
• J Xue, L Xu, H Zhu, M Bai, X Li, Z Zhao, H Zhong, G Cheng, X Li, F Hu, Y Su CD14+CD16- monocytes are the main precursors of osteoclasts in rheumatoid arthritis via expressing Tyro3TK Arthritis Research & Therapy, 2020-09-21;22(1):221. 2020-09-21 [PMID: 32958023] (Flow Cytometry, Human)
• Alisha Holtzhausen, William Harris, Eric Ubil, Debra M. Hunter, Jichen Zhao, Yuewei Zhang et al. TAM Family Receptor kinase inhibition reverses MDSC-mediated suppression and augments anti-PD-1 therapy in melanoma. Cancer Immunology Research 2019-10-01 [PMID: 31451482]
• BA Chua, JA Ngo, K Situ, CM Ramirez, H Nakano, K Morizono Protein S and Gas6 induce efferocytosis of HIV-1-infected cells Virology, 2018-01-02;515(0):176-190. 2018-01-02 [PMID: 29304470] (Flow Cytometry, Human)
• Kasikara C, Kumar S, Kimani S, Tsou W, Geng K, Davra V, Sriram G, Devoe C, Nguyen K, Antes A, Krantz A, Rymarczyk G, Wilczynski A, Empig C, Freimark B, Gray M, Schlunegger K, Hutchins J, Kotenko S, Birge R Phosphatidylserine Sensing by TAM Receptors Regulates AKT-Dependent Chemoresistance and PD-L1 Expression. Mol Cancer Res, 2017-02-09;15(6):753-764. 2017-02-09 [PMID: 28184013] (Flow Cytometry, Human)
• Axl is required for TGF-?2-induced dormancy of prostate cancer cells in the bone marrow Sci Rep, 2016-11-07;6(0):36520. 2016-11-07 [PMID: 27819283] (Flow Cytometry, Human)
• Anna Malawista, Xiaomei Wang, Mark Trentalange, Heather G. Allore, Ruth R. Montgomery Coordinated expression of Tryo3, Axl, and Mer receptors in macrophage ontogeny Macrophage (Houst) 2016-10-04 [PMID: 27695708]
• Zheng S, Hedl M, Abraham C TAM receptor-dependent regulation of SOCS3 and MAPKs contributes to proinflammatory cytokine downregulation following chronic NOD2 stimulation of human macrophages. J Immunol, 2015-01-07;194(4):1928-37. 2015-01-07 [PMID: 25567680] (Flow Cytometry, Human)
• Gould WR, Baxi SM, Schroeder R, Peng YW, Leadley RJ, Peterson JT, Perrin LA Gas6 receptors Axl, Sky and Mer enhance platelet activation and regulate thrombotic responses. J. Thromb. Haemost., 2005-02-23;3(4):733-41. 2005-02-23 [PMID: 15733062] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: TYRO3
• Uniprot:
  • Q06418()