DRP1 Antibody [Alexa Fluor® 647]

Product Discontinued

DRP1 Antibody [Alexa Fluor® 647] Summary

• Immunogen: A synthetic peptide made to an N-terminal region within residues 1-100 of the human DRP1 protein. [Swiss-Prot# O00429]
• Localization: Cytoplasm; cytosol. Intracytoplasmic membrane; peripheral membrane protein. Mainly cytosolic. Also membrane-associated. Localizes to mitochondia at spots of division events. Associated with peroxisomal membranes, it is recruited in part by PEX11B.
• Predicted Species: Primate (100%), Hamster (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DNM1L
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
• Application Notes: This DRP1 antibody is useful for Immunohistochemistry-paraffin embedded sections, Immunocytochemistry/Immunofluorescence and Western blot analysis where a band is seen at ~81 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. In ICC/IF, cytoplasmic staining was observed in HeLa cells.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 23658809).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for DRP1 Antibody [Alexa Fluor® 647]

• DLP1
• DNM1L
• DRP1
• DRP1Dnm1p/Vps1p-like protein
• DVLP
• DVLPEC 3.6.5.5
• Dymple
• DYMPLEDYNIV-11
• dynamin 1-like
• Dynamin family member proline-rich carboxyl-terminal domain less
• dynamin-1-like protein
• Dynamin-like protein 4
• Dynamin-like protein IV
• Dynamin-like protein
• Dynamin-related protein 1
• FLJ41912
• HdynIV
• VPS1

Background

DRP1 is a member of the dynamin GTPase superfamily, which that contributes to mitochondrial division in mammalian cells. DRP1 plays this important role in mitochondrial fission at steady state and during apoptosis. DRP1 is required for proper cellular distribution of mitochondria, and in mutant neurons, mitochondria are largely absent from synapses. Therefore, DRP1 provides a genetic tool to assess the role of mitochondria at synapses.

The DRP1 gene has 3 alternatively spliced transcripts encoding different isoforms, which are alternatively polyadenylated.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DNM1L