DRP1 Antibody

Images

 
Western Blot: DRP1 Antibody [NB110-55237] - Staining of DRP-1 in mouse embryonic fibroblast post-nuclear extracts. Image courtesy of D. Chan California Institute of Technology.
Immunocytochemistry/ Immunofluorescence: DRP1 Antibody [NB110-55237] - DRP1 antibody (1:50) was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight ...read more
Immunohistochemistry: DRP1 Antibody [NB110-55237] - Staining of renal tubular epithelium and visceral epithelial cells of glomeruli in Human kidney cortex. 40X magnification.
Simple Western: DRP1 Antibody [NB110-55237] - Simple Western lane view shows a specific band for DRP1 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ha, PmSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

DRP1 Antibody Summary

Immunogen
A synthetic peptide made to an N-terminal region within residues 1-100 of the human DRP1 protein. [Swiss-Prot# O00429]
Localization
Cytoplasm; cytosol. Intracytoplasmic membrane; peripheral membrane protein. Mainly cytosolic. Also membrane-associated. Localizes to mitochondia at spots of division events. Associated with peroxisomal membranes, it is recruited in part by PEX11B.
Predicted Species
Hamster (100%), Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DNM1L
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Simple Western 1:50
  • Immunocytochemistry/Immunofluorescence 1:50-1:400
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Immunoprecipitation
Application Notes
This DRP1 antibody is useful for Immunohistochemistry-paraffin embedded sections, Immunocytochemistry/Immunofluorescence and Western blot analysis where a band is seen at ~81 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. In ICC/IF, cytoplasmic staining was observed in HeLa cells. Use in Immunoprecipitation reported in scientific literature (PMID 25299344).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Positive Control
Kidney Lysate (NB820-59231)
DRP1 Lysate (NBL1-09966)
Control Peptide
DRP1 Peptide (NB110-55237PEP)
Publications
Read Publications using
NB110-55237 in the following applications:

  • 2 publications
  • IHC
    4 publications
  • IP
    1 publication
  • WB
    10 publications

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 23658809).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DRP1 Antibody

  • DLP1
  • DRP1Dnm1p/Vps1p-like protein
  • DVLPEC 3.6.5.5
  • Dymple
  • DYMPLEDYNIV-11
  • dynamin 1-like
  • Dynamin family member proline-rich carboxyl-terminal domain less
  • dynamin-1-like protein
  • Dynamin-like protein 4
  • Dynamin-like protein IV
  • Dynamin-like protein
  • Dynamin-related protein 1
  • FLJ41912
  • HDYNIV
  • VPS1

Background

DRP1 (dynamin-related protein 1) is a highly conserved large GTPase protein which implicates in several important structural features of mitochondria, including mitochondrial shape, size, distribution, remodeling and maintenance. It functions in mitochondrial as well as peroxisomal division, and facilitates membrane fission via oligomerization into ring-like structures that wrap around fission site to constrict/split the membranes through a GTP hydrolysis-dependent mechanism. For normal brain development, DRP1 plays an essential role by mediating developmentally-regulated apoptosis during neural tube development. It is also required for a normal rate of cytochrome c release or caspase activation during apoptosis and for mitochondrial fission during mitosis, programmed necrosis execution and also for vesicle transport. Moreover, it is associated with mitochondrial structural and functional alterations such as fragmentation, phosphorylation, SUMOylation, ubiquitination and cell death. Drp1 interacts with amyloid beta, causing abnormal mitochondrial dynamics and neuronal damage in Alzheimer's disease patients, and defective DRP1 is also linked to encephalopathy, lethal, due to defective mitochondrial and peroxisomal fission (EMPF).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB110-55237
Species: Hu, Mu, Rt, Ha, Pm
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, IP

Publications for DRP1 Antibody (NB110-55237)(14)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: ICC/IF, IHC, IP, WB.


Filter By Application
ICC/IF
(2)
IHC
(4)
IP
(1)
WB
(10)
All Applications
Filter By Species
Human
(7)
Mouse
(5)
Rat
(1)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using NB110-55237 Applications Species
Zou P, Liu L, Zheng LD et al. Coordinated Upregulation of Mitochondrial Biogenesis and Autophagy in Breast Cancer Cells: The Role of Dynamin Related Protein-1 and Implication for Breast Cancer Treatment Oxid Med Cell Longev Sep 1 2016 [PMID: 27746856] (IHC, Human) IHC Human
Chen SD, Zhen YY, Lin JW et al. Dynamin-Related Protein 1 Promotes Mitochondrial Fission and Contributes to The Hippocampal Neuronal Cell Death Following Experimental Status Epilepticus CNS Neurosci Ther Aug 31 2016 [PMID: 27577016] (WB, Human) WB Human
Ishizu N, Yui D, Hebisawa A et al. Impaired striatal dopamine release in homozygous Vps35 D620N knock-in mice. Hum. Mol. Genet. Aug 25 2016 [PMID: 27562021] (WB, Mouse) WB Mouse
Fu J, Yu Hm, Chiu Sy et al. Disruption of SUMO-Specific Protease 2 Induces Mitochondria Mediated neurodegeneration. PLoS Genet. 2014 Oct 01 [PMID: 25299344] (WB, IHC, IP, Mouse) WB, IHC, IP Mouse
Montaigne D, Marechal X, Coisne A et al. Myocardial Contractile Dysfunction is Associated with Impaired Mitochondrial Function and Dynamics in Type 2 Diabetic but not in Obese Patients. Circulation. 2014 Jun 13 [PMID: 24928681] (WB, Human) WB Human
Stavru F, Palmer AE, Wang C et al. Atypical mitochondrial fission upon bacterial infection. Proc Natl Acad Sci U S A. 2013 Oct 1 [PMID: 24043775] (ICC/IF, Human) ICC/IF Human
Wappler EA, Institoris A, Dutta S et al. Mitochondrial Dynamics Associated with Oxygen-Glucose Deprivation in Rat Primary Neuronal Cultures. PLoS One 2013 May 2 [PMID: 23658809] (WB, Rat) WB Rat
Marsboom G, Toth PT, Ryan JJ, Hong Z, Wu X, Fang YH, Thenappan T, Piao L, Zhang HJ, Pogoriler J, Chen Y, Morrow E, Weir EK, Rehman J, Archer SL. Dynamin-related protein 1-mediated mitochondrial mitotic fission permits hyperproliferation of vascular smooth muscle cells and offers a novel therapeutic target in pulmonary hypertension. Circ Res;110(11):1484-97. 2012 May 25. [PMID: 22511751] (WB, IHC, ICC/IF, Human) WB, IHC, ICC/IF Human
Manczak M, Sesaki H, Kageyama Y, Reddy PH. Dynamin-related protein 1 heterozygote knockout mice do not have synaptic and mitochondrial deficiencies Biochim Biophys Acta 2012 Feb 23 [PMID: 22387883] (WB, Mouse) WB Mouse
Rehman J, Zhang HJ, Toth PT, Zhang Y, Marsboom G, Hong Z, Salgia R, Husain AN, Wietholt C, Archer SL. Inhibition of mitochondrial fission prevents cell cycle progression in lung cancer. FASEB J;26(5):2175-86. 2012 May. [PMID: 22321727] (IHC, WB, Human) IHC, WB Human
Show All 14 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DRP1 Antibody (NB110-55237) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-55237AF405
Alexa Fluor 488 Labeled NB110-55237AF488
Alexa Fluor 647 Labeled NB110-55237AF647
Alexa Fluor 700 Labeled NB110-55237AF700
Biotin Labeled NB110-55237B
DyLight 405 Labeled NB110-55237V
DyLight 488 Labeled NB110-55237G
DyLight 550 Labeled NB110-55237R
DyLight 650 Labeled NB110-55237C
FITC Labeled NB110-55237F
HRP Labeled NB110-55237H

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Blogs on DRP1.

Dynamin-related Protein 1 (DRP1) in Mitochondria and Apoptosis.
Dynamin-related Protein 1 (DRP1) is known to function in mitochondrial and peroxisomal division and mediate membrane fission through oligomerization into ring-like structure and sever the mitochondrial membrane, through a GTP hydrolysis-dependent mech...  Read full blog post.

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Bioinformatics

Gene Symbol DNM1L