Western Blot: DRP1 Antibody [NB110-55237] - Staining of DRP-1 in mouse embryonic fibroblast post-nuclear extracts. Image courtesy of D. Chan California Institute of Technology.
Immunocytochemistry/ Immunofluorescence: DRP1 Antibody [NB110-55237] - DRP1 antibody (1:50) was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight ...read more
Immunohistochemistry: DRP1 Antibody [NB110-55237] - Staining of renal tubular epithelium and visceral epithelial cells of glomeruli in Human kidney cortex. 40X magnification.
Simple Western: DRP1 Antibody [NB110-55237] - Simple Western lane view shows a specific band for DRP1 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
A synthetic peptide made to an N-terminal region within residues 1-100 of the human DRP1 protein. [Swiss-Prot# O00429]
Cytoplasm; cytosol. Intracytoplasmic membrane; peripheral membrane protein. Mainly cytosolic. Also membrane-associated. Localizes to mitochondia at spots of division events. Associated with peroxisomal membranes, it is recruited in part by PEX11B.
Hamster (100%), Primate (100%). Backed by our 100% Guarantee.
Immunogen affinity purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
This DRP1 antibody is useful for Immunohistochemistry-paraffin embedded sections, Immunocytochemistry/Immunofluorescence and Western blot analysis where a band is seen at ~81 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. In ICC/IF, cytoplasmic staining was observed in HeLa cells. Use in Immunoprecipitation reported in scientific literature (PMID 25299344).
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Rat reactivity reported in scientific literature (PMID: 23658809).
Packaging, Storage & Formulations
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Tris-Glycine and 0.15M NaCl
0.05% Sodium Azide
Immunogen affinity purified
Alternate Names for DRP1 Antibody
Dynamin family member proline-rich carboxyl-terminal domain less
Dynamin-like protein 4
Dynamin-like protein IV
Dynamin-related protein 1
DRP1 (dynamin-related protein 1) is a highly conserved large GTPase protein which implicates in several important structural features of mitochondria, including mitochondrial shape, size, distribution, remodeling and maintenance. It functions in mitochondrial as well as peroxisomal division, and facilitates membrane fission via oligomerization into ring-like structures that wrap around fission site to constrict/split the membranes through a GTP hydrolysis-dependent mechanism. For normal brain development, DRP1 plays an essential role by mediating developmentally-regulated apoptosis during neural tube development. It is also required for a normal rate of cytochrome c release or caspase activation during apoptosis and for mitochondrial fission during mitosis, programmed necrosis execution and also for vesicle transport. Moreover, it is associated with mitochondrial structural and functional alterations such as fragmentation, phosphorylation, SUMOylation, ubiquitination and cell death. Drp1 interacts with amyloid beta, causing abnormal mitochondrial dynamics and neuronal damage in Alzheimer's disease patients, and defective DRP1 is also linked to encephalopathy, lethal, due to defective mitochondrial and peroxisomal fission (EMPF).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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