Mitofusin 1 Antibody (3C9)

Images

 
Western Blot: Mitofusin 1 Antibody (3C9) [H00055669-M04] - Analysis of MFN1 expression in HepG2.
Western Blot: Mitofusin 1 Antibody (3C9) [H00055669-M04] - MFN1 monoclonal antibody (M04), clone 3C9 Western Blot analysis of MFN1 expression in HeLa ( Cat # L013V1 ).
Western Blot: Mitofusin 1 Antibody (3C9) [H00055669-M04] - Western blot analysis of MFN1 over-expressed 293 cell line, cotransfected with MFN1 Validated Chimera RNAi or non-transfected control. Blot probed with ...read more
Western Blot: Mitofusin 1 Antibody (3C9) [H00055669-M04] - Analysis of MFN1 expression in transfected 293T cell line by MFN1 monoclonal antibody (M04), clone 3C9. Lane 1: MFN1 transfected lysatE (84.2 KDa). Lane 2: ...read more
ELISA: Mitofusin 1 Antibody (3C9) [H00055669-M04] - Detection limit for recombinant GST tagged MFN1 is 0.3 ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IP, KD
Clone
3C9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Mitofusin 1 Antibody (3C9) Summary

Immunogen
MFN1 (AAH40557, 1 a.a. ~ 741 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAEPVSPLKHFVLAKKAITAIFDQLLEFVTEGSHFVEATYKNPELDRIATEDDLVEMQGYKDKLSIIGEVLSRRHMKVAFFGRTSSGKSSVINAMLWDKVLPSGIGHITNCFLSVEGTDGDKAYLMTEGSDEKKSVKTVNQLAHALHMDKDLKAGCLVRVFWPKAKCALLRDDLVLVDSPGTDVTTELDSWIDKFCLDADVFVLVANSESTLMNTEKHFFHKVNERLSKPNIFILNNRWDASASEPEYMEDVRRQHMERCLHFLVEELKVVNALEAQNRIFFVSAKEVLSARKQKAQGMPESGVALAEGFHARLQEFQNFEQIFEECISQSAVKTKFEQHTIRAKQILATVKNIMDSVNLAAEDKRHYSVEEREDQIDRLDFIRNQMNLLTLDVKKKIKEVTEEVANKVSCAMTDEICRLSVLVDEFCSEFHPNPDVLKIYKSELNKHIEDGMGRNLADRCTDEVNALVLQTQQEIIENLKPLLPAGIQDKLHTLIPCKKFDLSYNLNYHKLCSDFQEDIVFRFSLGWSSLVHRFLGPRNAQRVLLGLSEPIFQLPRSLASTPTAPTTPATPDNASQEELMITLVTGLASVTSRTSMGIIIVGGVIWKTIGWKLLSVSLTMYGALYLYERLSWTTHAKERAFKQQFVNYATEKLRMIVSSTSANCSHQVKQQIATTFARLCQQVDITQKQLEEEIARLPKEIDQLEKIQNNSKLLRNKAVQLENELENFTKQFLPSSNEES
Marker
Mitochondrial Fusion Marker
Specificity
MFN1 - mitofusin 1. This antibody may cross react with MFN2 protein.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MFN1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunoprecipitation
  • Knockdown Validated
  • Western Blot 1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for RNAi validation and ELISA. Flow Cytometry was reported in scientific literature.
Publications
Read Publications using
H00055669-M04 in the following applications:

  • 1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Mitofusin 1 Antibody (3C9)

  • DKFZp762F247
  • EC 3.6.5
  • EC 3.6.5.-
  • FLJ20693
  • Fzo homolog
  • hfzo1
  • hfzo2
  • MFN1
  • MGC41806
  • mitochondrial transmembrane GTPase Fzo-1
  • mitochondrial transmembrane GTPase FZO-2
  • Mitofusin 1
  • mitofusin-1
  • putative transmembrane GTPase
  • Transmembrane GTPase MFN1

Background

The protein encoded by this gene is a mediator of mitochondrial fusion. This protein and mitofusin 2 are homologs of the Drosophila protein fuzzy onion (Fzo). They are mitochondrial membrane proteins that interact with each other to facilitate mitochondrial targeting.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Mitofusin 1 Antibody (H00055669-M04)(26)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: FLOW, WB.


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FLOW
(1)
WB
(1)
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(1)
Mouse
(2)
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Showing Publications 1 - 10 of 26. Show All 26 Publications.
Publications using H00055669-M04 Applications Species
Zhao Y, Sun X, Hu D et al. ATAD3A oligomerization causes neurodegeneration by coupling mitochondrial fragmentation and bioenergetics defects. Nat Commun. 2019-03-26 [PMID: 30914652]
Zhou Q, Li H, Li Y et al. Inhibiting neddylation modification alters mitochondrial morphology and reprograms energy metabolism in cancer cells. JCI Insight. 2019-05-04 [PMID: 30668548]
Ferreira JCB, Campos JC, Qvit N et al. A selective inhibitor of mitofusin 1-beta IIPKC association improves heart failure outcome in rats. Nat Commun. 2019-04-09 [PMID: 30659190]
Ishikawa K, Yamamoto S, Hattori S et al. Acquired expression of mutant Mitofusin 2 causes progressive neurodegeneration and abnormal behavior. J Neurosci. 2019-02-03 [PMID: 30606759]
Wei Q, Sun H, Song S et al. microRNA-668 represses MTP18 to preserve mitochondrial dynamics in ischemic acute kidney injury. J Clin Invest 2018-11-12 [PMID: 30325740]
Furuya N, Kakuta S, Sumiyoshi K et al. NDP52 interacts with mitochondrial RNA poly(A) polymerase to promote mitophagy. EMBO Rep 2018-10-11 [PMID: 30309841]
Vukotic M, Nolte H, Konig T et al. Acylglycerol Kinase Mutated in Sengers Syndrome Is a Subunit of the TIM22 Protein Translocase in Mitochondria. Mol Cell 2017-07-14 [PMID: 28712724]
Guedouari H, Daigle T, Scorrano L, Hebert-Chatelain E. Sirtuin 5 protects mitochondria from fragmentation and degradation during starvation. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 2016-11-15 [PMID: 28340937]
Chen M, Chen Z, Wang Y et al. Mitophagy receptor FUNDC1 regulates mitochondrial dynamics and mitophagy. Autophagy 2016-04-02 [PMID: 27050458]
Shiba-Fukushima K, Inoshita T, Hattori N, Imai Y. Lysine 63-linked Polyubiquitination Is Dispensable for Parkin-mediated Mitophagy. J Biol Chem. 2014-11-28 [PMID: 25336644]
Show All 26 Publications.

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Product General Protocols

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol MFN1
Entrez
OMIM
Uniprot