DR3/TNFRSF25 Antibody [Biotin]

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

DR3/TNFRSF25 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse DR3/TNFRSF25
  Gln31-Phe199
  Accession # AAK11256
• Specificity: Detects mouse DR3/TNFRSF25 in Western blots. In this format, approximately 5% cross-reactivity with recombinant human DR3/TNFRSF25 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TNFRSF25
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DR3/TNFRSF25 Antibody [Biotin]

• APO3
• Apo-3
• Apoptosis-inducing receptor AIR
• Apoptosis-mediating receptor DR3
• Apoptosis-mediating receptor TRAMP
• Death receptor 3
• DR3
• DR3member 12 (translocatingchain-association membrane protein)
• LARD
• Lymphocyte-associated receptor of death
• Protein WSL
• Protein WSL-1
• TNFRSF25
• TRAMP
• tumor necrosis factor receptor superfamily, member 25
• WSL
• WSL1
• WSL-1

Background

Death receptor 3 (DR3), also known as TNFRSF25, LARD, WSL-1, APO3, TRAMP, and TR3, is a 55 kDa TNF receptor superfamily protein that is predominantly expressed by lymphocytes. TNF receptor superfamily members have varying numbers of extracellular cysteine-rich domains (CRDs) with conserved cysteine spacing (1, 2). DR3 contains four CRDs and one cytoplasmic death domain (3, 4). Alternative splicing of mouse DR3 generates an isoform that lacks the fourth CRD and a secreted isoform that consisits of only the extracellular domain (ECD) (3). Human DR3 exists in at least eleven alternate splice forms (5). Within the ECD, mouse and human DR3 share 59% amino acid (aa) sequence identity. DR3 shares 20% - 28% aa sequence identity with the ECD of death domain receptors DR5, DR6, EDAR, Fas, NGF R, and TNF RI. Naïve B and T cells preferentially express truncated soluble isoforms of DR3, whereas stimulated lymphocytes preferentially express transmembrane DR3 (5). TL1A/TNFSF15, a high affinity DR3 ligand which also exists in membrane bound and soluble forms, is expressed by activated endothelial cells and T cells (6, 7). TL1A additionally binds to DcR3/TNFRSF6B, a soluble decoy receptor that interferes with DR3 activation (8). DR3 signaling triggers either apoptosis or NF kappa B-induced anti-apoptotic effects depending on the cellular setting (9). Apoptosis is partially impaired during negative selection of thymocytes in DR3-null mice (10). TL1A interactions with DR3 augment T cell proliferation and proinflammatory cytokine secretion (6, 7, 11, 12). DR3 is upregulated by inflammatory stimulation of CCR9⁺ T cells, a T cell subset important in mucosal immunity (11). T cell and macrophage DR3 expression is prominent in several inflammatory disorders such as Crohn’s disease, inflammatory bowel disease, and atherosclerosis (7, 11-15). DR3 activation on IFN-gamma treated THP-1 cells induces the production of TNF-alpha , CXCL8, CCL2, MMP-1, -9, and -13 (14, 15).

1. Fas, S.C. et al. (2006) Curr. Dir. Autoimmun. 9:1.
2. Aggarwal, B.B. (2003) Nat. Rev. Immunol. 3:745.
3. Wang, E.C.Y. et al. (2001) Immunogenetics 53:59.
4. Borysenko, C.W. et al. (2005) Biochem. Biophys. Res. Commun. 328:794.
5. Screaton, G.R. et al. (1997) Proc. Natl. Acad. Sci. USA 94:4615.
6. Migone, T.S. et al. (2002) Immunity 16:479.
7. Prehn, J.L. et al. (2004) Clin. Immunol. 112:66.
8. Yang, C-R. et al. (2004) Cancer Res. 64:1122.
9. Wen, L. et al. (2003) J. Biol. Chem. 278:39251.
10. Wang, E.C.Y. et al. (2001) Mol. Cell. Biol. 21:3451.
11. Papadakis, K.A. et al. (2005) J. Immunol. 174:4985.
12. Papadakis, K.A. et al. (2004) J. Immunol. 172:7002.
13. Bamias, G. et al. (2003) J. Immunol. 171:4868.
14. Kim, S-H. et al. (2001) Jpn. Circ. J. 65:136.
15. Kang, Y-J. et al. (2005) Cytokine 29:229.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DR3/TNFRSF25 Antibody (BAF2437)(4)

Publications using BAF2437

• JR Ferdinand, AC Richard, F Meylan, A Al-Shamkha, RM Siegel Cleavage of TL1A Differentially Regulates Its Effects on Innate and Adaptive Immune Cells J. Immunol., 2018-01-15;0(0):. 2018-01-15 [PMID: 29335258] (Flow Cytometry, Mouse)
• Richard A, Tan C, Hawley E, Gomez-Rodriguez J, Goswami R, Yang X, Cruz A, Penumetcha P, Hayes E, Pelletier M, Gabay O, Walsh M, Ferdinand J, Keane-Myers A, Choi Y, O'Shea J, Al-Shamkhani A, Kaplan M, Gery I, Siegel R, Meylan F The TNF-family ligand TL1A and its receptor DR3 promote T cell-mediated allergic immunopathology by enhancing differentiation and pathogenicity of IL-9-producing T cells. J Immunol, 2015-03-18;194(8):3567-82. 2015-03-18 [PMID: 25786692] (IHC, Mouse)
• Twohig J, Marsden M, Cuff S, Ferdinand J, Gallimore A, Perks W, Al-Shamkhani A, Humphreys I, Wang E The death receptor 3/TL1A pathway is essential for efficient development of antiviral CD4(+) and CD8(+) T-cell immunity. FASEB J, 2012-05-16;26(8):3575-86. 2012-05-16 [PMID: 22593543] (Flow Cytometry, Mouse)
• Taraban VY, Ferdinand JR, Al-Shamkhani A Expression of TNFRSF25 on conventional T cells and Tregs. J. Clin. Invest., 2011-02-01;121(2):463-4. 2011-02-01 [PMID: 21285518] (Flow Cytometry, Mouse)

Review for DR3/TNFRSF25 Antibody (BAF2437) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer Flow Mouse 01/12/2015

Summary

• Application: Flow Cytometry
• Sample Tested: See PMID 22593543
• Species: Mouse

Bioinformatics

• Gene Symbol: TNFRSF25
• Uniprot:
  • AAK11256()