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DPPA4 Antibody [Biotin]

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NTera‑2 human testicular embryonic carcinoma cell line was stained with Sheep Anti-Human DPPA4 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3674, filled histogram) or isotype control antibody (Catalog # BAF020), followed by Streptavidin-Phycoerythrin (Catalog # F0040). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
NTera‑2 human testicular embryonic carcinoma cell line was stained with Sheep Anti-Human DPPA4 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3674, filled histogram) or isotype control ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow
Clonality
Polyclonal
Host
Sheep
Conjugate
Biotin
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Unconjugated
AF3674
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF3674
Carrier Free
AF3674
Carrier Free
AF3674-SP
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DPPA4 Antibody [Biotin] Summary

Immunogen
E. coli-derived recombinant human DPPA4
Met1-Glu304
Accession # Q7L190
Specificity
Detects human DPPA4 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse DPPA4 is observed and less than 1% cross-reactivity with recombinant human DPPA5 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
DPPA4
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
Readout System

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DPPA4 Antibody [Biotin]

  • developmental pluripotency associated 4
  • developmental pluripotency-associated protein 4
  • DPPA4
  • FLJ10713,2410091M23Rik

Background

DPPA4 is a nuclear SAP-domain protein whose expression is restricted to pluripotent cells, undifferentiated embryonal carcinomas and germ cell tumors. It is considered a marker for embryonic stem cells. Human DPPA4 shares 51% amino acid sequence homology with mouse DPPA4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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DPPA4 Antibody
[Biotin]
BAF3674
DPPA4 Antibody [Biotin]
Species: Hu
Applications: WB, Flow

Publications for DPPA4 Antibody (BAF3674) (0)

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Secondary Antibodies

 

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Biotin BAF3674
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Bioinformatics

Gene Symbol DPPA4
Uniprot