DPPA4 Antibody [Unconjugated]

NTera‑2 human testicular embryonic carcinoma cell line was stained with Sheep Anti-Human DPPA4 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3674, filled histogram) or isotype control antibody (Catalog #5‑001‑A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

DPPA4 was detected in immersion fixed human plasmid-derived induced pluripotent stem cells (iPSK3) using Sheep Anti-Human DPPA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3674) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Identification of USP26 as a negative regulator of pluripotency. a Schematic of experimental strategy for screening essential Usps for the generation of iPSCs. Dox-inducible OSKM-transgenic MEFs were plated, transfected with 48 individual mouse Usp shRNA lentiviral vectors to knockdown Usps, and stained for AP+ iPSC colonies after 12 days of Dox treatment. b Quantification of AP+ colonies after 12 days of OSKM induction in MEFs transduced with shRNA, targeting members of the mouse Usp family, p < 0.001 compared to control shRNA. c Quantification of AP+ colonies after 12 days of OSKM induction in MEFs transduced with mouse Usp26 shRNA, Usp20 shRNA, or control shRNA lentivirus, *p < 0.05 compared to control shRNA, **p < 0.01 compared to control shRNA. d Quantification of AP+ colonies after 12 days of OSKM induction in MEFs transduced with pLtet-O (tetracycline-inducible) mouse Usp26, Usp20, or empty vector (EV) lentivirus, **p < 0.01 compared to EV. e Images of AP staining of iPSC colonies after 12 days’ OSKM induction in MEFs transduced with control shRNA, Usp26 shRNA, EV, or pLtet-O lentivirus overexpressing Usp26. f Bright field (BF) and immunofluorescence microscopic images of Oct4, Nanog, and Dppa4 in Usp26 knockdown iPSCs. iPSC colonies were fixed, blocked, and stained with specific antibodies, followed by goat anti-mouse antibody-conjugated Texas Red. Nuclei were stained with DAPI. Scale bar, 100 µM. The data are presented as means ± SD from three independent experiments. b–d Two-way ANOVA for multiple comparisons Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28839133), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, CyTOF-ready, Flow, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

DPPA4 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human DPPA4
  Met1-Glu304
  Accession # Q7L190
• Specificity: Detects human DPPA4 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse DPPA4 is observed and less than 1% cross-reactivity with recombinant human DPPA5 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: DPPA4
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Immunocytochemistry 5-15 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DPPA4 Antibody [Unconjugated]

• developmental pluripotency associated 4
• developmental pluripotency-associated protein 4
• DPPA4
• FLJ10713,2410091M23Rik

Background

DPPA4 is a nuclear SAP-domain protein whose expression is restricted to pluripotent cells, undifferentiated embryonal carcinomas and germ cell tumors. It is considered a marker for embryonic stem cells. Human DPPA4 shares 51% amino acid sequence homology with mouse DPPA4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DPPA4
• Uniprot:
  • Q7L190()