Doublecortin Antibody

Images

 
Western Blot: Doublecortin Antibody [NBP1-32662] - Non-transfected (-) and Doublecortin-transfected (+, ) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Doublecortin ...read more
Immunocytochemistry/ Immunofluorescence: Doublecortin Antibody [NBP1-32662] - Staining of neurons derived from human ESC with a 1:200 dilution of anti-doublecortin antibody colocalizes with anti-MAP2 antibody (green). ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, ChSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.76 mg/ml

Order Details

Doublecortin Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Doublecortin. The exact sequence is proprietary.
Localization
Cytoplasm
Marker
Immature Neuronal Marker
Predicted Species
Mouse (99%), Rat (100%), Chicken (94%), Bovine (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DCX
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
Theoretical MW
49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.76 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Doublecortin Antibody

  • doublecortin
  • Doublin
  • FLJ51296
  • Lissencephalin-X
  • Lis-X
  • X-linked (doublecortin)

Background

In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The protein encoded by this gene is a cytoplasmic protein which appears to direct neuronal migration by regulating the organization and stability of microtubules. The encoded protein contains two doublecortin domains, which bind microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in this gene are a cause of X-linked lissencephaly. Multiple transcript variants encoding at least three different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-25208
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
MAB140
Species: Hu
Applications: Flow, IHC, CyTOF-ready, ELISA(Cap), ELISA(Det), Neut, ELISA(Sta)
AF-386-PB
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
DDX0131P-100
Species: Hu
Applications: Flow, Func, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
H00003117-M01
Species: Hu
Applications: WB, ELISA, PLA
NBP1-49045
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, CyTOF-ready, InhibTFunc
MAB1774
Species: Hu
Applications: Flow, CyTOF-ready, ICC
NB110-97871
Species: Mu
Applications: Flow, IHC, IHC-P, IP, CyTOF-ready
NB100-91662
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
AF4885
Species: Mu
Applications: WB, IP

Publications for Doublecortin Antibody (NBP1-32662) (0)

There are no publications for Doublecortin Antibody (NBP1-32662).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Doublecortin Antibody (NBP1-32662) (0)

There are no reviews for Doublecortin Antibody (NBP1-32662). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Doublecortin Antibody (NBP1-32662) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Doublecortin Products

Bioinformatics Tool for Doublecortin Antibody (NBP1-32662)

Discover related pathways, diseases and genes to Doublecortin Antibody (NBP1-32662). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Doublecortin Antibody (NBP1-32662)

Discover more about diseases related to Doublecortin Antibody (NBP1-32662).
 

Pathways for Doublecortin Antibody (NBP1-32662)

View related products by pathway.

PTMs for Doublecortin Antibody (NBP1-32662)

Learn more about PTMs related to Doublecortin Antibody (NBP1-32662).
 

Research Areas for Doublecortin Antibody (NBP1-32662)

Find related products by research area.

Blogs on Doublecortin

There are no specific blogs for Doublecortin, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Doublecortin Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol DCX