Doublecortin Antibody [Unconjugated]

Western blot shows lysates of human motor cortex tissue, human hypothalamus tissue, and human hippocampus tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse Doublecortin/DCX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF10025) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Doublecortin/DCX at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Doublecortin/DCX was detected in perfusion fixed frozen sections of mouse brain (hippocampus) using Sheep Anti-Human/Mouse Doublecortin/DCX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF10025) at 8 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated

Doublecortin Antibody [Unconjugated] Summary

• Immunogen: E.coli-derived recombinant human Doublecortin/DCX
  Met116-Thr293
  Accession # O43602
• Specificity: Detects human Doublecortin/DCX in direct ELISAs and Western blots. Detects mouse Doublecortin/DCX in immunohistochemistry.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Doublecortin Antibody [Unconjugated]

• DBCN
• Doublecortex
• Doublecortin
• Doublin
• FLJ51296
• Lissencephalin-X
• LISX
• Lis-X
• X-linked (doublecortin)

Background

Doublecortin (DCX) is a 365 aminoacids, 40 kDa microtubule-associated phosphoprotein (MAP) encoded by the DCX gene. DCX is an essential factor in neurogenesis, modulating, stabilizing and stimulating polymerization of microtubules (MTs) to ensure effective migration of neurons of the central and peripheral nervous system during embryonic and postnatal development. Mutations in this protein impairs neuronal migration leading to several pathological conditions.

DCX is very conserved across species. Human and non-human primates DCX proteins are 98-99% identical at the aminoacid level, and 100% identical at the sequences used for immunogen of AF10025; human, mouse and rat DCX aminoacid sequences are 98% identical.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Doublecortin Antibody (AF10025)(1)

Publication using AF10025

• Lei, C;Zhang, B;Yamaguchi, J;Tamura, R;Zhong, W;Cao, X;Liu, Y;Seki, M;Suzuki, Y;Suzuki, K;Tanida, I;Uchiyama, Y;Hisatsune, T; Dual inhibition of IRAK1/TAK1 signaling in astrocytes reduces accelerated mortality in human APOE4 knock-in APPswe/PSEN1dE9/P301S-Tau triple transgenic mouse model Journal of neuroinflammation 2025-10-21 [PMID: 41121315] (Immunohistochemistry, Mouse)

Bioinformatics

• Uniprot:
  • O43602()