Doublecortin Antibody [Alexa Fluor® 488]

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 488

Doublecortin Antibody [Alexa Fluor® 488] Summary

• Immunogen: E.coli-derived recombinant human Doublecortin/DCX
  Met116-Thr293
  Accession # O43602
• Specificity: Detects human Doublecortin/DCX in direct ELISAs and Western blots. Detects mouse Doublecortin/DCX in immunohistochemistry.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Doublecortin Antibody [Alexa Fluor® 488]

• DBCN
• Doublecortex
• Doublecortin
• Doublin
• FLJ51296
• Lissencephalin-X
• LISX
• Lis-X
• X-linked (doublecortin)

Background

Doublecortin (DCX) is a 365 aminoacids, 40 kDa microtubule-associated phosphoprotein (MAP) encoded by the DCX gene. DCX is an essential factor in neurogenesis, modulating, stabilizing and stimulating polymerization of microtubules (MTs) to ensure effective migration of neurons of the central and peripheral nervous system during embryonic and postnatal development. Mutations in this protein impairs neuronal migration leading to several pathological conditions.

DCX is very conserved across species. Human and non-human primates DCX proteins are 98-99% identical at the aminoacid level, and 100% identical at the sequences used for immunogen of AF10025; human, mouse and rat DCX aminoacid sequences are 98% identical.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.