Doublecortin Antibody (3E1) [PE]

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: 3E1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: PE

Doublecortin Antibody (3E1) [PE] Summary

• Description: This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
• Immunogen: Recombinant full length human Lis-A isoform of Doublecortin purified from E. coli. [UniProt# O43602]
• Localization: Cytoplasm. Cell projection. Note: Localizes at neurite tips.
• Marker: Immature Neuronal Marker
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: DCX
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Western Blot
• Application Notes: This Doublecortin (3E1) antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where bands can be seen at approximately 35 and 45 kDa.
  
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 49.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for Doublecortin Antibody (3E1) [PE]

• DBCN
• Doublecortex
• Doublecortin
• Doublin
• FLJ51296
• Lissencephalin-X
• LISX
• Lis-X
• X-linked (doublecortin)

Background

In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The protein encoded by this gene is a cytoplasmic protein which appears to direct neuronal migration by regulating the organization and stability of microtubules. The encoded protein contains two doublecortin domains, which bind microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in this gene are a cause of X-linked lissencephaly. Multiple transcript variants encoding at least three different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Doublecortin Antibody (NBP1-92684PE)(1)

Publication using NBP1-92684PE

• Wiatrak B, Kubis-Kubiak A, Piwowar A, Barg E PC12 Cell Line: Cell Types, Coating of Culture Vessels, Differentiation and Other Culture Conditions Cells 2020-04-14 [PMID: 32295099] (ICC/IF, Rat)

FAQs for Doublecortin Antibody (NBP1-92684PE). (Showing 1 - 1 of 1 FAQ).

1. Today we received an inquiry from our customer who is interested in #NBP1-92684PE, Anti-Doublecortin, Mouse-Mono(3E1), PE. The customer wants to know the concentration of this antibody. If you have any information, please let us know.
   • The core (unconjugated) product, NBP1-92684, is at a concentration of 1mg/ml. We assume that when we perform the conjugation reaction there is no more than a 20% loss of antibody, and therefore the concentration of NBP1-92684PE is 0.8 - 1mg/ml.

Bioinformatics

• Gene Symbol: DCX