DOT1L Antibody (OTI6E7)

Western Blot: DOT1L Antibody (6E7) [NBP2-46474] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOT1L.

Immunohistochemistry: DOT1L Antibody (6E7) [NBP2-46474] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: DOT1L Antibody (6E7) [NBP2-46474] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

• Reactivity: Hu
• Applications: WB, IHC
• Clone: OTI6E7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

DOT1L Antibody (OTI6E7) Summary

• Description: Novus Biologicals Mouse DOT1L Antibody (OTI6E7) (NBP2-46474) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1008-1424 of human DOT1L (NP_115871) produced in E.coli.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: DOT1L
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 164.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DOT1L Antibody (OTI6E7)

• DOT1L
• DOT1-like protein
• DOT1-like, histone H3 methyltransferase (S. cerevisiae)
• EC 2.1.1.43
• H3-K79-HMTase
• KIAA1814DKFZp586P1823
• KMT4
• KMT4H3 lysine-79 specific
• Lysine N-methyltransferase 4

Background

DOT1 is an evolutionarily conserved histone methyltransferase that methylates lysine-79 of histone H3 in the core domain. Unlike other histone methyltransferases, DOT1 does not contain a SET domain, and it specifically methylates nucleosomal histone H3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DOT1L