DOT1L Antibody (6A6) - Azide and BSA Free

Western Blot: DOT1L Antibody (6A6) [H00084444-M01] - DOT1L monoclonal antibody (M01), clone 6A6. Analysis of DOT1L expression in LNCaP.

Immunocytochemistry/Immunofluorescence: DOT1L Antibody (6A6) [H00084444-M01] - Analysis of monoclonal antibody to DOT1L on HeLa cell . Antibody concentration 10 ug/ml.

Sandwich ELISA: DOT1L Antibody (6A6) [H00084444-M01] - Detection limit for recombinant GST tagged DOT1L is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu, Fe
• Applications: WB, ELISA, ICC/IF
• Clone: 6A6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

DOT1L Antibody (6A6) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse DOT1L Antibody (6A6) - Azide and BSA Free (H00084444-M01) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. Anti-DOT1L Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: DOT1L (NP_115871, 3 a.a. ~ 108 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EKLELRLKSPVGAEPAVYPWPLPVYDKHHDAAHEIIETIRWVCEEIPDLKLAMENYVLIDYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNTRPSTGLLR
• Specificity: DOT1L - DOT1-like, histone H3 methyltransferase (S. cerevisiae)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: DOT1L
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against recombinant protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for DOT1L Antibody (6A6) - Azide and BSA Free

• DOT1L
• DOT1-like protein
• DOT1-like, histone H3 methyltransferase (S. cerevisiae)
• EC 2.1.1.43
• H3-K79-HMTase
• KIAA1814DKFZp586P1823
• KMT4
• KMT4H3 lysine-79 specific
• Lysine N-methyltransferase 4

Background

DOT1 is an evolutionarily conserved histone methyltransferase that methylates lysine-79 of histone H3 in the core domain. Unlike other histone methyltransferases, DOT1 does not contain a SET domain, and it specifically methylates nucleosomal histone H3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DOT1L Antibody (H00084444-M01)(1)

Publication using H00084444-M01

• Phillips TC, Wildt DE, Comizzoli P. Incidence of methylated histones H3K4 and H3K79 in cat germinal vesicles is regulated by specific nuclear factors at the acquisition of developmental competence during the folliculogenesis. J Assist Reprod Genet 2016-04-08 [PMID: 27059775]

Bioinformatics

• Gene Symbol: DOT1L
• Entrez:
  • 84444(Human)
• OMIM:
  • 607375(Human)
• Uniprot:
  • NP_115871(Human)