DOCK11 Antibody - BSA Free

Immunoprecipitation: DOCK11 Antibody [NB100-81663] - Detection of Human DOCK11 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-DOCK11 antibody NB100-81663 used for IP at 3 mcg/mg lysate. DOCK11 was also immunoprecipitated by rabbit anti-DOCK11 antibody NB100-81662, which recognizes an upstream epitope. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu
• Applications: IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

DOCK11 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit DOCK11 Antibody - BSA Free (NB100-81663) is a polyclonal antibody validated for use in IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 400 and 450 of human dedicator of cytokinesis 11 using the numbering given in entry NP_653259.3 (GeneID 139818).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DOCK11
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2 - 5 ug/mg lysate
• Application Notes: Immunoprecipitation. It is not recommended for western analysis.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DOCK11 Antibody - BSA Free

• ACG
• Activated Cdc42-associated guanine nucleotide exchange factor
• Activated Cdc42-associated guanine nucleotide exchange factor, ACG
• bB128O4.1
• bB128O4.1.1 (novel protein)
• dedicator of cytokinesis 11
• FLJ32122
• FLJ43653
• ZIZ2dedicator of cytokinesis protein 11
• zizimin2
• zizimin-2

Background

DOCK11 (dedicator of cytokinesis 11) is also known as zizimin-2. It is a DOCK180-related guanine nucleotide exchange factor (GEF) for the small GTPase, Cdc42. As a GEF, DOCK11 functions to catalyze the dissociation of GDP from the GTPase. Dissociation of GDP allows an exchange for GTP and subsequent downstream signaling by the GTPase. Alternate names for DOCK11 include activated Cdc42-associated guanine nucleotide exchange factor, ACG, and ZIZ2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DOCK11
• Entrez:
  • 139818(Human)
• Uniprot:
  • NP_653259.3(Human)