DOCK10 Antibody - BSA Free

Western Blot: DOCK10 Antibody [NB100-60669] - Whole cell lysate (50 ug) from Jurkat, LNCaP, and GaMG cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DOCK10 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Western Blot: DOCK10 Antibody [NB100-60669] - Detection of Human DOCK10 on HeLa whole cell lystate using NB100-60670.

Western Blot: DOCK10 Antibody [NB100-60669] - Absence of Zizimin proteins, but normal numbers of hematopoietic cells in KO mice. Western blotting for Ziz2 (A) or Ziz3 (DOCK10) (B) Protein lysates (30 ug/lane) were loaded and designated antibodies were reacted with the membranes. Replicated membranes were used for all antibodies. Zizimin proteins were absent in KO mice (8 weeks old, male). Image collected and cropped by CiteAb from the following publication (//www.immunityageing.com/content/12/1/1), licensed under a CC-BY license.

Western Blot: DOCK10 Antibody [NB100-60669] - Absence of Zizimin proteins, but normal numbers of hematopoietic cells in KO mice. (A-B) Western blotting for Ziz2 (A) or Ziz3 (B) Protein lysates (30 μg/lane) were loaded & designated antibodies were reacted with the membranes. Replicated membranes were used for all antibodies. Zizimin proteins were absent in KO mice (8 weeks old, male). (C-F) The numbers of hematopoietic cells were counted after the hemolytic reaction (C-E) or flow cytometric analysis (F). No significant difference was observed among the groups (C-F). Three mice (8 weeks old, female) per group from three independent experiments (one mouse per group per experiment) were used. 2KO: Ziz2 KO. 3KO: Ziz3 KO. Image collected & cropped by CiteAb from the following publication (//immunityageing.biomedcentral.com/articles/10.1186/s12979-015-0028-x), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu
• Applications: WB, IP, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

DOCK10 Antibody - BSA Free Summary

• Description: Novus Biologicals Knockout (KO) Validated Rabbit DOCK10 Antibody - BSA Free (NB100-60669) is a polyclonal antibody validated for use in WB and IP. Anti-DOCK10 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 100 and 150 of human dedicator of cytokinesis 10 using the numbering given in entry NP_055504.2 (GeneID 55619).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DOCK10
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-10 ug/mg lysate
  • Knockout Validated
  • Western Blot 1:2000-1:10000
• Theoretical MW: 249.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25729399)

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DOCK10 Antibody - BSA Free

• dedicator of cytokinesis 10
• dedicator of cytokinesis protein 10
• DKFZp781A1532
• DRIP2
• KIAA0694protein zizimin 3
• Nbla10300
• ZIZ3dopamine receptor interacting protein 2
• zizimin3
• zizimin-3

Background

DOCK10 is a potential Rho-guanine nucleotide exchange factor (Rho-GEF) that may function to activate small GTPases such as Rac and Cdc42. It is a member of the CZH (CDM-zizimin homology) family of proteins that contain two highly conserved domains, CZH1 and CZH2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DOCK10 Antibody (NB100-60669)(1)

Publication using NB100-60669

• Matsuda T, Yanase S, Takaoka A, Maruyama M. The immunosenescence-related gene Zizimin2 is associated with early bone marrow B cell development and marginal zone B cell formation Immun Ageing. 2015-03-02 [PMID: 25729399] (WB, Mouse)

FAQs for DOCK10 Antibody (NB100-60669). (Showing 1 - 1 of 1 FAQ).

1. The homology of DOCK9 and DOCK10 is high. Do the following antibodies cross-react with other DOCK family proteins? DOCK9 Antibody (NB500-265) and DOCK10 Antibody (NB100-60669).
   • I did a blast of the entire DOCK9 and DOCK10 sequences and they only have 50.54% identity between the two. In the amino acid ranges of the specific products NB500-265 and NB100-60669, I do not see any high similarity and would not predict these two proteins to cross react. We have not tested these products for cross reactivity with other DOCK proteins and cannot say with certainty that they will not cross react. Based on homology, I think it is unlikely.

Bioinformatics

• Gene Symbol: DOCK10
• Entrez:
  • 55619(Human)
• Uniprot:
  • Q96BY6(Human)