Dnmt3L Antibody - Azide and BSA Free

Western Blot: Dnmt3L Antibody [H00029947-D01P] - Analysis of DNMT3L expression in mouse liver.

Immunocytochemistry/Immunofluorescence: Dnmt3L Antibody [H00029947-D01P] - Analysis of antibody to DNMT3L on HeLa cell. Antibody concentration 30 ug/ml.

Western Blot: Dnmt3L Antibody [H00029947-D01P] - Analysis of DNMT3L expression in human liver.

Western Blot: Dnmt3L Antibody [H00029947-D01P] - Analysis of DNMT3L expression in transfected 293T cell line by DNMT3L polyclonal antibody.Lane 1: DNMT3L transfected lysate(43.50 KDa).Lane 2: Non-transfected lysate.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Dnmt3L Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit Dnmt3L Antibody - Azide and BSA Free (H00029947-D01P) is a polyclonal antibody validated for use in WB and ICC/IF. Anti-Dnmt3L Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: DNMT3L (AAH02560.1, 1 a.a. - 386 a.a.) full-length human protein. MAAIPALDPEAEPSMDVILVGSSELSSSVSPGTGRDLIAYEVKANQRNIEDICICCGSLQVHTQHPLFEGGICAPCKDKFLDALFLYDDDGYQSYCSICCSGETLLICGNPDCTRCYCFECVDSLVGPGTSGKVHAMSNWVCYLCLPSSRSGLLQRRRKWRSQLKAFYDRESENPLEMFETVPVWRRQPVRVLSLFEDIKKELTSLGFLESGSDPGQLKHVVDVTDTVRKDVEEWGPFDLVYGATPPLGHTCDRPPSWYLFQFHRLLQYARPKPGSPGPFFWMFVDNLVLNKEDLDVASRFLEMEPVTIPDVHGGSLQNAVRVWSNIPAIRSRHWALVSEEELSLLAQNKQSSKLAAKWPTKLVKNCFLPLREYFKYFSTELTSSL
• Specificity: Reacts with DNA (cytosine-5-)-methyltransferase 3-like.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DNMT3L
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: This antibody is reactive against tissue and transfected lysate in western blot, and as a detection antibody in ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Dnmt3L Antibody - Azide and BSA Free

• DNA (cytosine-5-)-methyltransferase 3-like
• DNA (cytosine-5)-methyltransferase 3-like
• human cytosine-5-methyltransferase 3-like protein, 10cytosine-5-methyltransferase 3-like protein
• MGC1090

Background

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases. This protein is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, this protein does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. Alternative splicing results in two transcript variants. An additional splice variant has been described but its biological validity has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Dnmt3L Antibody (H00029947-D01P)(2)

Publications using H00029947-D01P

• Kuhnel T, Heinz H, Utz N et al. A human somatic cell culture system for modelling gene silencing by transcriptional interference Heliyon 2020-01-01 [PMID: 32021933] (WB)
• Lue, JK;Prabhu, SA;Liu, Y;Gonzalez, Y;Verma, A;Mundi, PS;Abshiru, NA;Camarillo, JM;Mehta, S;Chen, EI;Qiao, C;Nandakumar, R;Cremers, S;Kelleher, NL;Elemento, O;Amengual, JE; Precision Targeting with EZH2 and HDAC Inhibitors in Epigenetically Dysregulated Lymphomas Clin. Cancer Res. 2019-04-12 [PMID: 30979734] (WB, Human)

Bioinformatics

• Gene Symbol: DNMT3L
• Entrez:
  • 29947(Human)
• Uniprot:
  • AAH02560.1(Human)