Dnmt3L Antibody (5H4A5) - BSA Free

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Western Blot: Dnmt3L Antibody (5H4A5) [NBP2-61755] - Analysis using DNMT3L mAb against human DNMT3L (AA: 147-386) recombinant protein. (Expected MW is 53.9 kDa)
Western Blot: Dnmt3L Antibody (5H4A5) [NBP2-61755] - Analysis using DNMT3L mAb against HEK293 (1) and DNMT3L (AA: 147-386)-hIgGFc transfected HEK293 (2) cell lysate.
ELISA: Dnmt3L Antibody (5H4A5) [NBP2-61755] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
5H4A5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

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Catalog# & Formulation Size Price

Dnmt3L Antibody (5H4A5) - BSA Free Summary

Immunogen
Purified recombinant fragment of human DNMT3L (AA: 147-386) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
DNMT3L
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Western Blot 1:500-1:2000
Theoretical MW
43.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Dnmt3L Antibody (5H4A5) - BSA Free

  • DNA (cytosine-5-)-methyltransferase 3-like
  • DNA (cytosine-5)-methyltransferase 3-like
  • human cytosine-5-methyltransferase 3-like protein, 10cytosine-5-methyltransferase 3-like protein
  • MGC1090

Background

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases, but is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, it does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.;

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Blogs on Dnmt3L.

The role of DNMT3B in the co-incidence of methyltransferase and tumor suppressor expression in malignancies
Epigenetics is the process of heritable change in gene activity despite alteration of the hosts DNA sequence, essentially causing a change in a phenotype without a change in the genotype of a host. To change the gene sequence without interfering w...  Read full blog post.

The role of DNMT3A in development
Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence.  Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation.  Gene silencing in DNA ...  Read full blog post.

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Bioinformatics

Gene Symbol DNMT3L