DNMT3A Antibody

Images

 
Western Blot: DNMT3A Antibody [NB100-265] - Analysis using NB100-265 to detect overexpressed DNMT3A level in HCT-116 cells. Lane 1: 40 ug of nuclear extract from untreated HCT-116 cells. Lane 2: 40 ug of Nuclear extract ...read more
Immunocytochemistry/ Immunofluorescence: DNMT3A Antibody [NB100-265] - Dnmt3a antibody was tested in Ntera-2 cells with Dylight 488 (green). Nuclei and beta-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more
Immunohistochemistry: DNMT3A Antibody [NB100-265] - Dnmt3a antibody was tested in human testis using DAB with hematoxylin counterstain.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

DNMT3A Antibody Summary

Immunogen
This DNMT3A Antibody was made from amino acids 10-118 of Human Dnmt3a [UniProt# Q9Y6K1]
Localization
Nuclear, cytoplasmic.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DNMT3A
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10-1:500
Application Notes
This Dnmt3a antibody is useful for Immunohistochemistry on paraffin-embedded sections, Immunoprecipitation, Immunocytochemistry/Immunofluorescence and Western Blot, where a band should be seen at ~120 kDa. NOTE: This antibody does not neutralize the enzymatic activity of its corresponding methyltransferase. In ICC/IF, nuclear and cytoplasmic staining was observed in Ntera-2 cells. In IHC-P, staining was observed in the cytoplasm of mouse testis. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Theoretical MW
120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
DNMT3A Overexpression Lysate
DNMT3A Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 4
using
NB100-265 in the following applications:

Publications
Read Publications using
NB100-265 in the following applications:

  • WB
    3 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.1% Sodium Azide
Purity
Unpurified

Alternate Names for DNMT3A Antibody

  • DNA (cytosine-5-)-methyltransferase 3 alpha
  • DNA (cytosine-5)-methyltransferase 3A
  • DNA cytosine methyltransferase 3A2
  • DNA Methyltransferase 3 Alpha
  • DNA methyltransferase HsaIIIA
  • DNA MTase HsaIIIA
  • DNMT3A
  • DNMT3A2
  • EC 2.1.1.37
  • M.HsaIIIA
  • TBRS

Background

Methylation of cytosine residues is essential for mammalian development by regulating gene expression, gene silencing and genomic imprinting (1). This process is controlled by the enzyme DNA (cytosine-5)-methyltransferase 3A (DNMt3a), which is encoded by the DNMT3A gene and has a predicted molecular weight of 102 kDa. DNA methyltransferases including DNMt3a are involved in de novo cytosine methylation and maintenance of embryonic and somatic cells through the transfer of methyl groups to specific CpG structures in DNA (2). Somatic mutations in DNMT3A are responsible for cytogenetically normal acute myeloid leukemia (CN-AML). Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA are linked with cancer (3,4). Mutations in the DNMT3A gene have also been found to cause DNMT3A overgrowth syndrome and myelodysplastic syndromes.

References

1. Thakur, A., Mackin, S. J., Irwin, R. E., O'Neill, K. M., Pollin, G., & Walsh, C. (2016). Widespread recovery of methylation at gametic imprints in hypomethylated mouse stem cells following rescue with DNMT3A2. Epigenetics Chromatin, 9, 53. doi:10.1186/s13072-016-0104-2

2. Ravichandran, M., Lei, R., Tang, Q., Zhao, Y., Lee, J., Ma, L.,... Dawlaty, M. M. (2019). Rinf Regulates Pluripotency Network Genes and Tet Enzymes in Embryonic Stem Cells. Cell Rep, 28(8), 1993-2003.e1995. doi:10.1016/j.celrep.2019.07.080

3. Pang, Y., Liu, J., Li, X., Xiao, G., Wang, H., Yang, G.,... Ren, H. (2018). MYC and DNMT3A-mediated DNA methylation represses microRNA-200b in triple negative breast cancer. J Cell Mol Med, 22(12), 6262-6274. doi:10.1111/jcmm.13916

4. Xia, L., Huang, W., Bellani, M., Seidman, M. M., Wu, K., Fan, D.,... Baylin, S. B. (2017). CHD4 Has Oncogenic Functions in Initiating and Maintaining Epigenetic Suppression of Multiple Tumor Suppressor Genes. Cancer Cell, 31(5), 653-668.e657. doi:10.1016/j.ccell.2017.04.005

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DNMT3A Antibody (NB100-265)(11)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.


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WB
(3)
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(6)
Mouse
(3)
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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB100-265 Applications Species
Yang J, Wu R, Li W et al. The triterpenoid corosolic acid blocks transformation and epigenetically reactivates Nrf2 in TRAMP-C1 prostate cells. Mol. Carcinog. 2017 Dec 16 [PMID: 29247555] (Mouse) Mouse
Selvakumar T, Gjidoda A, Hovde SL, Henry RW. Regulation of Human RNA Polymerase III Transcription by DNMT1 and DNMT3a DNA Methyltransferases J Biol Chem 2012 Mar 2 [PMID: 22219193] (WB, Human) WB Human
Kaminskyy V, Surova O, Vaculova A, Zhivotovsky B. Combined inhibition of DNA methyltransferase and histone deacetylase restores caspase-8 expression and sensitizes SCLC cells to TRAIL. Carcinogenesis. 2011 Jul 18. [PMID: 21771726]
Al-Salihi M, Yu M, Burnett DM et al. The depletion of DNA methyltransferase-1 and the epigenetic effects of 5-aza-2'deoxycytidine (decitabine) are differentially regulated by cell cycle progression. Epigenetics 2011 Aug [PMID: 21725200] (Human) Human
Shikauchi Y et al. SALL3 interacts with DNMT3A and shows the ability to inhibit CpG island methylation in hepatocellular carcinoma. Mol Cell Biol;29(7):1944-58. 2009 Apr. [PMID: 19139273]
Fanelli M, Caprodossi S, Ricci-Vitiani L et al. Loss of pericentromeric DNA methylation pattern in human glioblastoma is associated with altered DNA methyltransferases expression and involves the stem cell compartment. Oncogene 2008 Jan 10 [PMID: 17653095] (Human) Human
Reynolds PA et al. Tumor suppressor p16INK4A regulates polycomb-mediated DNA hypermethylation in human mammary epithelial cells. J Biol Chem;281(34):24790-802. 2006 Aug 25. [PMID: 16766534]
La Salle S, Trasler JM. Dynamic expression of DNMT3a and DNMT3b isoforms during male germ cell development in the mouse. Dev Biol 2006 Aug 1 [PMID: 16725135] (Mouse) Mouse
Robert MF et al. DNMT1 is required to maintain CpG methylation and aberrant gene silencing in human cancer cells. Nat Genet;33(1):61-5. 2003 Jan. [PMID: 12496760] (WB, Human) WB Human
Weisenberger, DJ et al. Identification and characterization of alternatively spliced variants of DNA methyltransferase 3a in mammalian cells. Gene;298(1):91-9. 2002 Sep 18. [PMID: 12406579] (Human, Mouse) Human, Mouse
Show All 11 Publications.

Review for DNMT3A Antibody (NB100-265) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-265:
Filter by Applications
ICC
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry DNMT3A NB100-265
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reviewed by:
Anonymous
ICC Human 11/18/2017
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Summary

ApplicationImmunocytochemistry
Sample Tested fibroblasts
SpeciesHuman
Lotc-3

Comments

CommentsDiluted 1:50 in 1X PBS/1% BSA/0.3% Triton™ X-100, 16 hours at 4C incubation.
2 hours (25C) secondary (1.5:1000 dilution)

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DNMT3A Antibody (NB100-265). (Showing 1 - 2 of 2 FAQs).

  1. I'll try to experiment ChIP for using the following antibody. Dnmt3a Antibody (NB100-265) Please tell me a concentration it.
    • NB100-265 is provided in whole antisera and the concentration of antibody is not available.
  2. Does this antibody recognize the short (723 aa / 81 kD) or the long (912 aa/102 kD) form of the DNA methytransferase 3A?
    • The immunogen sequence used for Dnmt3a antibody (NB100-265) is from the amino acids 10-118 of human Dnmt3a which does not match the amino acid sequence of the short forms of this target (i.e. 723 AA Isoform-2 or 166 AA Isoform-3). Therefore this antibody should not be able to detect the short forms of Dnmt3a. In our testing on HCT-116 cells, we have observed the target bands at approximately 120 kDa, and no bands were observed at approximately 82kD or 17kD positions.

Control Lysate(s)

Secondary Antibodies

 

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Blogs on DNMT3A.

Chromatin reader domains of DNMT-targeting protein, UHRF1, are responsible for cancerous DNA hypermethylation
By Jamshed Arslan, Pharm. D., PhD. DNA methylation represses transcription of many genes, including tumor suppressor genes. A protein called UHRF1 recruits DNA methyltransferases (DNMTs) to establish and maintain DNA...  Read full blog post.

Eat responsibly: Epigenetic downregulation of Ankrd26 gene by long-term high-fat intake promotes obesity and inflammation
By Jamshed Arslan Pharm.D. White adipose tissue (WAT) represents the primary site to store energy in humans. WAT’s endocrine regulation of energy balance is controlled by nutritional status, exercise, and hormones l...  Read full blog post.

The role of DNMT3A in development
Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence.  Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation.  Gene silencing in DNA ...  Read full blog post.

Go Ahead! Make My DNA
DNA methylation plays a critical role the long-term silencing of transcription and is essential for processes such as embryonic development, germline differentiation, and tissue maturation. Dnmt3a is a member of the C5-methyltransferase family that re...  Read full blog post.

DNMT's: An Overview of 3 DNA Methyltransferases
DNA methyltransferases catalyze the transfer of the methyl group from S-andenosyl methionine (SAM) to DNA. Such methylation has wide ranging function in the cell, including organismal development and cell differentiation. In cancer, abnormal hypermeth...  Read full blog post.

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Recent Reviews

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1
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Anonymous
11/18/2017
Application: ICC
Species: Human

Bioinformatics

Gene Symbol DNMT3A
Entrez
Uniprot