Dnmt2 Antibody

Western Blot: Dnmt2 Antibody [NBP3-18663] - Figure 2. Western blot analysis using the antibody directed against Dnmt2 (1) Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against mouse Dnmt2 , diluted 1:1000 in BSA/PBS- Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (47 kDa) is indicated on the right.

ELISA: Dnmt2 Antibody [NBP3-18663] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against mouse Dnmt2 . The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:21,000.

• Reactivity: Mu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Dnmt2 Antibody Summary

• Description: Novus Biologicals Rabbit Dnmt2 Antibody (NBP3-18663) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Polyclonal antibody raised in rabbit against mouse Dnmt2 (DNA methyltransferase 2), using a KLHconjugated synthetic peptide containing an amino acid sequence from the N-terminal part of the protein (1).
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRDMT1
• Purity: Whole Antiserum

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:1000
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antiserum
• Preservative: 0.05% Sodium Azide
• Purity: Whole Antiserum

Alternate Names for Dnmt2 Antibody

• DMNT2
• DNA (cytosine-5-)-methyltransferase 2
• DNA (cytosine-5)-methyltransferase-like protein 2
• DNA methyltransferase homolog HsaIIP
• DNA methyltransferase-2
• DNA MTase homolog HsaIIP
• DNMT2
• EC 2.1.1
• EC 2.1.1.29
• M.HsaIIP
• MHSAIIP
• PUMET
• RNMT1
• tRNA (cytosine-5-)-methyltransferase
• tRNA aspartic acid methyltransferase 1

Background

tRNA aspartic acid methyltransferase 1 (TRDMT1), also known as DNMT2, is a DNA methyltransferase homolog that does not methylate DNA. Instead DNMT-2 methylates cytosine-38 in the anticodon loop of aspartic acid transfer RNA. Spec ifically, DNMT-2 methylates cytosine 38 in the anticodon loop of tRNA(Asp)

DNMT2 antibodies are useful for RNA research, cell replication studies, and research on certian cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRDMT1
• Uniprot:
  • O14717(Mouse)