DNMT1 Assay Kit (Colorimetric)

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Product Details

Summary
Applications QFN
Suitable Sample Type
Nuclear extracts
Standard Curve Range
1 to 20 ug sample
Sensitivity
1 ug sample

Order Details

DNMT1 Assay Kit (Colorimetric) Summary

Description
DNMT1 Assay Kit is use for measuring the amount of DNMT1.
Standard Curve Range
1 to 20 ug sample
Sensitivity
1 ug sample
Assay Type
Colorimetric
Sample Volume
2- 6 uL
Kit Type
Assay Kit (Colorimetric)
Gene
DNMT1

Applications/Dilutions

Dilutions
  • Quantification

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. 8-Well Assay Strip (with Frame)
  2. Blocking Buffer
  3. D1 (10X Wash Buffer
  4. D2 (Assay Buffer)
  5. D3 (DNMT1 Standard, 20 µg/mL)
  6. D4 (Capture Antibody, 500 µg/mL)
  7. D5 (Detection antibody 200 µg/mL)
  8. D6 (Developing Solution)
  9. D7 (Stop Solution)

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for DNMT1 Assay Kit (Colorimetric)

  • AIM
  • CXXC9
  • CXXC9CXXC finger protein 9
  • CXXC-type zinc finger protein 9
  • DNA (cytosine-5-)-methyltransferase 1
  • DNA (cytosine-5)-methyltransferase 1
  • DNA methyltransferase 1
  • DNA methyltransferase HsaI
  • DNA MTase HsaI
  • DNMT
  • DNMT1
  • EC 2.1.1.37
  • FLJ16293
  • m.HsaI
  • MCMT
  • MCMTMGC104992

Background

DNMT1 Assay Kit is use for measuring the amount of DNMT1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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Blogs on DNMT1.

The role of DNMT3B in the co-incidence of methyltransferase and tumor suppressor expression in malignancies
Epigenetics is the process of heritable change in gene activity despite alteration of the hosts DNA sequence, essentially causing a change in a phenotype without a change in the genotype of a host. To change the gene sequence without interfering w...  Read full blog post.

The role of DNMT3A in development
Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence.  Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation.  Gene silencing in DNA ...  Read full blog post.

Controlling Epigenetic Signaling with Dnmt1 and Dnmt3b
Dnmt1 belongs to the C5-methyltransferase family that repairs cytosines in dsDNA using a nucleophilic attack mechanism. Dnmt1 is the most abundant mammalian DNA methyltransferase. It is the key methylation maintenance enzyme for both DNA replication/r...  Read full blog post.

PCNA Antibodies: Marking Cell Proliferation & DNA Replication
Proliferating Cell Nuclear Antigen (PCNA), also known as the polymerase delta auxiliary protein, is a nuclear protein essential for DNA replication as well as DNA excision and mismatch repair pathways. It has a large role in cell cycle regulation and ...  Read full blog post.

DNMT's: An Overview of 3 DNA Methyltransferases
DNA methyltransferases catalyze the transfer of the methyl group from S-andenosyl methionine (SAM) to DNA. Such methylation has wide ranging function in the cell, including organismal development and cell differentiation. In cancer, abnormal hypermeth...  Read full blog post.

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Bioinformatics

Gene Symbol DNMT1