UHRF1 Antibody (1A1B1) - BSA Free

Western Blot: UHRF1 Antibody (1A1B1) [NBP2-61838] - Analysis using UHRF1 mAb against HEK293 (1) and UHRF1 (AA: 616-755)-hIgGFc transfected HEK293 (2) cell lysate.

Immunocytochemistry/Immunofluorescence: UHRF1 Antibody (1A1B1) [NBP2-61838] - Analysis of Hela cells using UHRF1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.

Immunohistochemistry: UHRF1 Antibody (1A1B1) [NBP2-61838] - Analysis of paraffin-embedded esophageal cancer tissues using UHRF1 mouse mAb with DAB staining.

Flow Cytometry: UHRF1 Antibody (1A1B1) [NBP2-61838] - Analysis of MCF-7 cells using UHRF1 mouse mAb (green) and negative control (red).

Western Blot: UHRF1 Antibody (1A1B1) [NBP2-61838] - Analysis using UHRF1 mAb against human UHRF1 (AA: 616-755) recombinant protein. (Expected MW is 41.8 kDa)

Western Blot: UHRF1 Antibody (1A1B1) [NBP2-61838] - Analysis using UHRF1 mouse mAb against MCF-7 (1) and Hela (2) cell lysate.

Immunocytochemistry/Immunofluorescence: UHRF1 Antibody (1A1B1) [NBP2-61838] - Analysis of Hela cells using UHRF1 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Immunohistochemistry: UHRF1 Antibody (1A1B1) [NBP2-61838] - Analysis of paraffin-embedded bladder cancer tissues using UHRF1 mouse mAb with DAB staining.

ELISA: UHRF1 Antibody (1A1B1) [NBP2-61838] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clone: 1A1B1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

UHRF1 Antibody (1A1B1) - BSA Free Summary

• Description: Novus Biologicals Mouse UHRF1 Antibody (1A1B1) - BSA Free (NBP2-61838) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human UHRF1 (AA: 616-755) expressed in E. Coli.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: UHRF1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 89.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for UHRF1 Antibody (1A1B1) - BSA Free

• E3 ubiquitin-protein ligase UHRF1
• EC 6.3.2
• EC 6.3.2.-
• FLJ21925
• hNp95
• hUHRF1
• HuNp95
• ICBP90
• ICBP90NP95
• Inverted CCAAT box-binding protein of 90 kDa
• NP95
• Nuclear protein 95
• Nuclear zinc finger protein Np95
• RING finger protein 106
• RNF106
• RNF106MGC138707
• Transcription factor ICBP90
• Ubiquitin-like PHD and RING finger domain-containing protein 1
• ubiquitin-like with PHD and ring finger domains 1
• ubiquitin-like, containing PHD and RING finger domains, 1
• Ubiquitin-like-containing PHD and RING finger domains protein 1
• UHRF1

Background

This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. It is regarded as a hub protein for the integration of epigenetic information. This gene is up-regulated in various cancers, and it is therefore considered to be a therapeutic target. Multiple transcript variants encoding different isoforms have been found for this gene. A related pseudogene exists on chromosome 12.;

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UHRF1