| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56519SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF6110-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84328-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59344-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98763-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26614-50ul | |||
![Western Blot: DNMT1 Antibody (60B1220.1) [NB100-56519] - Analysis of Dnmt1 in 2102EP (human embryonic carcinoma) in the A) absence and B) presence of immunizing peptide, recombinant human Dnmt1 protein in the C) absence and D) presence of immunizing peptide, NIH 3T3 (mouse embryonic fribroblast) in the E) absence and F) presence of immunizing peptide, and D3 (mouse embryonic stem cell) in the G) absence and H) presence of immunizing peptide using Dnmt1 antibody. this antibody. 5 ug/ml for testing the human cell line and 0.1 ug/ml was used for testing the recombinant protein and the mouse cell lines. ’s goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/DNMT1-Antibody-60B1220-1-Western-Blot-NB100-56519-img0003.jpg "Western Blot: DNMT1 Antibody (60B1220.1) [NB100-56519] - Analysis of Dnmt1 in 2102EP (human embryonic carcinoma) in the A) absence and B) presence of immunizing peptide, recombinant human Dnmt1 protein in the C) absence and D) presence of immunizing peptide, NIH 3T3 (mouse embryonic fribroblast) in the E) absence and F) presence of immunizing peptide, and D3 (mouse embryonic stem cell) in the G) absence and H) presence of immunizing peptide using Dnmt1 antibody. this antibody. 5 ug/ml for testing the human cell line and 0.1 ug/ml was used for testing the recombinant protein and the mouse cell lines. ’s goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunocytochemistry/Immunofluorescence: DNMT1 Antibody (60B1220.1) [NB100-56519] - Dnmt1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Antibody dilution of 1:10 was used. Image objective 40x.](http://images.novusbio.com/fullsize/DNMT1-Antibody-60B1220-1-Immunocytochemistry-Immunofluorescence-NB100-56519-img0006.jpg)
![Immunohistochemistry-Paraffin: DNMT1 Antibody (60B1220.1) [NB100-56519] - Tissue section of human hepatocellular carcinoma at 1:50 dilution.](http://images.novusbio.com/fullsize/DNMT1-Antibody-60B1220-1-Immunohistochemistry-Paraffin-NB100-56519-img0007.jpg)
![Flow Cytometry: DNMT1 Antibody (60B1220.1) [NB100-56519] - An intracellular stain was performed on HeLa cells with DNMT1 [60B1220.1] Antibody NB100-56519AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/DNMT1-Antibody-60B1220-1-Flow-Cytometry-NB100-56519-img0010.jpg)
![Flow Cytometry: DNMT1 Antibody (60B1220.1) [NB100-56519] - Analysis of PE conjugate of NB100-56519. An intracellular stain was performed on HeLa cells with DNMT1 antibody (60B1220.1) NB100-56519PE (blue) and a matched isotype control NBP2-27287PE (orange). Cells were fixed with 4% PFA and then permeablized with 0](http://images.novusbio.com/fullsize/DNMT1-Antibody-60B1220-1-Flow-Cytometry-NB100-56519-img0009.jpg)
![Simple Western: DNMT1 Antibody (60B1220.1) [NB100-56519] - Simple Western lane view shows a specific band for Dnmt1 in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/DNMT1-Antibody-60B1220-1-Simple-Western-NB100-56519-img0008.jpg)
![Immunoprecipitation: DNMT1 Antibody (60B1220.1) [NB100-56519] - The PCNAS228I mutation differentially affects PCNA binding to PIP box-containing proteins. GST-PIP pull down of His-S-PCNAWT or PCNAS228I. Figure shows Coomassie stained gel of representative pull down (top) and anti-PCNA western blot of the same samples diluted 1:20 (bottom). Amount of 'input' loaded for Coomassie is equivalent to 1%, 'Glut. beads' (Glutathione sepharose 4B beads) is equivalent to 25%. Molecular weight markers are indicated. Image collected and cropped by CiteAb from the following publication (//linkinghub.elsevier.com/retrieve/pii/S1568786416304013), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/DNMT1-Antibody-60B1220-1-Immunoprecipitation-NB100-56519-img0011.jpg)
![Western Blot: DNMT1 Antibody (60B1220.1) - BSA Free [NB100-56519] - WA does not alter DNMTs gene & protein expression(A) Effect of WA on DNMT1, DNMT3A & DNMT3B gene expression in MDA-MB-231 & MCF-7 cells normalized to select housekeeping genes & relative to DMSO-treated MCF-7 sample (2-delta delta Ct) as determined by Human Epigenetic Chromatin Modification Enzyme qPCR Array as previously described. Bars represent DNMT1, DNMT3A & DNMT3B relative mRNA (mean ± SEM) expression of at least two independent experiments. (B) Effect of WA on DNMT1, DNMT3A & DNMT3B protein expression in MDA-MB-231 & MCF-7 cells, as compared to to beta -Actin protein levels. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28467815), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56519_mouse-monoclonal-dnmt1-antibody-60b1220-1-310202415171327.jpg)
![Chromatin Immunoprecipitation: DNMT1 Antibody (60B1220.1) - BSA Free [NB100-56519] - Epigenetic changes & protein binding at Ankrd26 promoter in eAT from mice upon 22 weeks of HFD.ChIP of DNMT1, DNMT3a, DNMT3b (a) & MBD2 (b) binding at Ankrd26 promoter region (−553 bp/−348 bp). (c) MNase for Nuc-2 (−257 bp/−198 bp) & Nuc-1 (−84 bp/−25 bp) occupancy at Ankrd26 promoter. (d) ChIP for acetyl-H4 enrichment at Nuc-2 & Nuc-1. (e) ChIP of RNA Pol II binding at Ankrd26 TSS (+16 bp/+159 bp). (a,b) & (d,e), ChIP enrichment is relative to Input chromatin. (a–e), results are mean ± SD from three independent experiments. **p < 0.01 & ***p < 0.001 vs STD. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep43526), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56519_mouse-monoclonal-dnmt1-antibody-60b1220-1-310202415304276.jpg)
![Western Blot: DNMT1 Antibody (60B1220.1) - BSA Free [NB100-56519] - Verification of the expression of the proteins identified.(A) Validation of the SILAC results of selected proteins in immunoblots of protein extracts from the bladder cancer cells analyzed. The results validated the expression levels of proteins identified by the proteomic approach, including differentially & non-differentially expressed candidates. Antibodies displaying a single predominant band at the expected molecular weights were accepted: & alpha -tubulin, was used as the loading control. GSN, Gelsolin; Cul3, Cullin 3; IPO9. Importin 9; EGFR, Epidermal Growth Factor Receptor; NUP133, Nucleoporin 133; HSP70, Heat Shock Protein 70kDa; MCM6, Minichromosome Maintenance Complex Component 6; RCC1, Regulator of Chromosome Condensation 1; BCAS2, Breast Carcinoma Amplified Sequence 2; DNM, Dynamin; NPM, Nucleophosmin; DCTN, Dynactin; CALR, Calreticulin; MAPK, Mitogen-Activated Protein Kinase; DDX21, DEAD (Asp-Glu-Ala-Asp) box polypeptide 21; CDC2: Cell Division Cycle 2; DNMT1, DNA (cytosine-5)-Methyltransferase 1; MSH6, MutS Homolog 6; RAB14, GTPase Rab14; VDAC, Voltage-Dependent Anion Channel; CK18, Cytokeratin 18; CALD, Caldesmon; CD44, CD44 antigen isoform 1 precursor 2; EZR, Ezrin; MSN, Moesin; ANXA2, Annexin A2; CPNE3, Copine 3; FLNA, Filamin A; CAV1, Caveolin 1. Western Blots were scanned & analyzed using alpha -tubulin as normalizing control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23308193), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56519_mouse-monoclonal-dnmt1-antibody-60b1220-1-3102024165721.jpg)
![Western Blot: DNMT1 Antibody [NB100-392] - Detection of human DNMT1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DNMT1 antibody NB100-392 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/DNMT1-Antibody-Western-Blot-NB100-392-img0019.jpg "Western Blot: DNMT1 Antibody [NB100-392] - Detection of human DNMT1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DNMT1 antibody NB100-392 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: DNMT1 Antibody [NB100-392] - Dnmt1 in mouse brain cells. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/DNMT1-Antibody-Western-Blot-NB100-392-img0002.jpg)
![Immunoprecipitation: DNMT1 Antibody [NB100-392] - Detection of human DNMT1 in HeLa nuclear extracts [50 ug/lane (A), 10 ug input, or 1 mg NB 100-392 IP (B)] using NB 100-392. DNMT1 immunoprecipitated with NB 100-392 and detected with NB 100-392 using 0.2 ug/ml. 2 second ECL exposure.](http://images.novusbio.com/fullsize/DNMT1-Antibody-Immunoprecipitation-NB100-392-img0004.jpg)
![Immunoprecipitation: DNMT1 Antibody [NB100-392] - Detection of human DNMT1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DNMT1 antibody NB100-392 used for IP at 3 ug per reaction. For blotting immunoprecipitated DNMT1, NB100-392 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/DNMT1-Antibody-Immunoprecipitation-NB100-392-img0020.jpg)
![Western Blot: DNMT1 Antibody (4G11-C7) [NBP2-59344] - Western Blot analysis of Human H1299 cell lysate showing detection of DNMT1 protein using Mouse Anti-DNMT1 Monoclonal Antibody, Clone 4G11-C7 (SMC-201). Primary Antibody: Mouse Anti-DNMT1 Monoclonal Antibody (SMC-201) at 1:1000.](http://images.novusbio.com/fullsize/DNMT1-Antibody-4G11-C7-Western-Blot-NBP2-59344-img0001.jpg "Western Blot: DNMT1 Antibody (4G11-C7) [NBP2-59344] - Western Blot analysis of Human H1299 cell lysate showing detection of DNMT1 protein using Mouse Anti-DNMT1 Monoclonal Antibody, Clone 4G11-C7 (SMC-201). Primary Antibody: Mouse Anti-DNMT1 Monoclonal Antibody (SMC-201) at 1:1000.")
![Immunoprecipitation: DNMT1 Antibody (1K5K8) [NBP3-15856] - Immunoprecipitation of DNMT1 from 300 ug extracts of Jurkat cells was performed using 3 ug of DNMT1 Rabbit mAb . Rabbit IgG isotype control was used to precipitate the Control IgG sample. IP samples were eluted with 1X reducing Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using DNMT1 Rabbit mAb at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-15856_rabbit-dnmt1-mab-1k5k8-52202517251949.jpg "Immunoprecipitation: DNMT1 Antibody (1K5K8) [NBP3-15856] - Immunoprecipitation of DNMT1 from 300 ug extracts of Jurkat cells was performed using 3 ug of DNMT1 Rabbit mAb . Rabbit IgG isotype control was used to precipitate the Control IgG sample. IP samples were eluted with 1X reducing Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using DNMT1 Rabbit mAb at a dilution of 1:1000.")
![<b>Genetic Strategies Validation. </b>Western Blot: DNMT1 Antibody (1K5K8) [NBP3-15856] - Western blot analysis of lysates from wild type (WT) and DNMT1 knockdown (KD) 293T cells, using [KD Validated] DNMT1 Rabbit mAb at1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-15856_rabbit-dnmt1-mab-1k5k8-52202517152774.jpg)
![Western Blot: DNMT1 Antibody (1K5K8) [NBP3-15856] - Western blot analysis of various lysates using [KD Validated] DNMT1 Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit Exposure time: 30 s.](http://images.novusbio.com/fullsize/nbp3-15856_rabbit-dnmt1-mab-1k5k8-52202517284967.jpg)
![Immunohistochemistry-Paraffin: DNMT1 Antibody [NBP2-98763] - Immunochemical staining of human DNMT1 in human placenta with rabbit polyclonal antibody (1:500, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/DNMT1-Antibody-Immunohistochemistry-Paraffin-NBP2-98763-img0003.jpg "Immunohistochemistry-Paraffin: DNMT1 Antibody [NBP2-98763] - Immunochemical staining of human DNMT1 in human placenta with rabbit polyclonal antibody (1:500, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: DNMT1 Antibody [NBP2-98763] - Immunochemical staining of human DNMT1 in human brain with rabbit polyclonal antibody (1:500, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/DNMT1-Antibody-Immunohistochemistry-Paraffin-NBP2-98763-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: DNMT1 Antibody [NBP2-98763] - Immunofluorescence staining of DNMT1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98763 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to nucleus.](http://images.novusbio.com/fullsize/nbp2-98763_rabbit-polyclonal-dnmt1-antibody-23520252135111.png)
![Immunohistochemistry: DNMT1 Antibody (1A9) [NBP3-26614] - Image of DNMT1 Antibody (1A9) diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26614_rabbit-dnmt1-mab-1a9-262202415475617.jpg "Immunohistochemistry: DNMT1 Antibody (1A9) [NBP3-26614] - Image of DNMT1 Antibody (1A9) diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunocytochemistry/Immunofluorescence: DNMT1 Antibody (1A9) [NBP3-26614] - Staining of HepG2 Cells with DNMT1 Antibody (1A9) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26614_rabbit-dnmt1-mab-1a9-282202412475138.jpg)
![DNMT1 Assay/Inhibitor Screening Assay Kit [KA1515] - The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.](http://images.novusbio.com/fullsize/DNMT1-Assay-Inhibitor-Screening-Assay-Kit-KA1515-img0001.jpg "DNMT1 Assay/Inhibitor Screening Assay Kit [KA1515] - The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.")
![DNMT1 Assay/Inhibitor Screening Assay Kit [KA1515] - Positive control by using nuclear extract from MCF-7 cells.](http://images.novusbio.com/fullsize/DNMT1-Assay-Inhibitor-Screening-Assay-Kit-KA1515-img0002.jpg)
![Western Blot: DNMT1 Antibody (A3A8-R) [NBP3-32274] - Western blot analysis of DNMT1 on different lysates with Mouse anti-DNMT1 antibody (NBP3-32274) at 1/1,000 dilution. Lane 1: HeLa cell lysate, 20 ug/Lane Lane 2: 293T cell lysate, 20 ug/Lane Lane 3: HepG2 cell lysate, 20 ug/Lane Lane 4: Jurkat cell lysate, 20 ug/Lane Predicted band size: 183 kDa Observed band size: 183 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32274) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32274_mouse-dnmt1-mab-a3a8-r-278202416285256.jpg "Western Blot: DNMT1 Antibody (A3A8-R) [NBP3-32274] - Western blot analysis of DNMT1 on different lysates with Mouse anti-DNMT1 antibody (NBP3-32274) at 1/1,000 dilution. Lane 1: HeLa cell lysate, 20 ug/Lane Lane 2: 293T cell lysate, 20 ug/Lane Lane 3: HepG2 cell lysate, 20 ug/Lane Lane 4: Jurkat cell lysate, 20 ug/Lane Predicted band size: 183 kDa Observed band size: 183 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32274) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: DNMT1 Antibody (A3A8-R) [NBP3-32274] - Immunocytochemistry analysis of HeLa cells labeling DNMT1 with Mouse anti-DNMT1 antibody (NBP3-32274) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-DNMT1 antibody (NBP3-32274) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32274_mouse-dnmt1-mab-a3a8-r-2920241192358.jpg)
![Immunohistochemistry-Paraffin: DNMT1 Antibody (DNMT1/2061) - Azide and BSA Free [NBP3-08482] - Formalin-fixed, paraffin-embedded human Placenta stained with DNMT1 Mouse Monoclonal Antibody (DNMT1/2061).](http://images.novusbio.com/fullsize/DNMT1-Antibody-DNMT1-2061-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08482-img0001.jpg "Immunohistochemistry-Paraffin: DNMT1 Antibody (DNMT1/2061) - Azide and BSA Free [NBP3-08482] - Formalin-fixed, paraffin-embedded human Placenta stained with DNMT1 Mouse Monoclonal Antibody (DNMT1/2061).")
![Immunohistochemistry-Paraffin: DNMT1 Antibody (DNMT1/2061) - Azide and BSA Free [NBP3-08482] - Formalin-fixed, paraffin-embedded human Tonsil stained with DNMT1 Mouse Monoclonal Antibody (DNMT1/2061).](http://images.novusbio.com/fullsize/DNMT1-Antibody-DNMT1-2061-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08482-img0002.jpg)