DNMT1 Antibody

Images

 
Western Blot: DNMT1 Antibody [nb100-264] - Western Blot: DNMT1 Antibody [NB100-264] - Analysis using NB100-264 to detect DNMT1 levels in T24 degrees Cells. Lane 1: 50 ugs of total protein lysate from untreated T24 ...read more
Immunocytochemistry/ Immunofluorescence: DNMT1 Antibody [nb100-264] - Immunocytochemistry/ Immunofluorescence: DNMT1 Antibody [NB100-264] - The Dnmt1 antibody was tested in HeLa cells at a 1:50 dilution against Dylight ...read more
Immunohistochemistry-Paraffin: DNMT1 Antibody [nb100-264] - Immunohistochemistry-Paraffin: DNMT1 Antibody [NB100-264] - IHC staining of Dnmt1 in mouse spleen using DAB with hematoxylin counterstain.
Immunohistochemistry-Paraffin: DNMT1 Antibody [nb100-264] - Immunohistochemistry-Paraffin: DNMT1 Antibody [NB100-264] - IHC staining of Dnmt1 in mouse small intestine using DAB with hematoxylin counterstain.
Simple Western: DNMT1 Antibody [nb100-264] - Simple Western: DNMT1 Antibody [NB100-264] - Simple Western lane view shows a specific band for Dnmt1 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

DNMT1 Antibody Summary

Immunogen
Contained within amino acids 1-125 of the N-terminus of human Dnmt1 [UniProt# P26358]
Localization
Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DNMT1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western 1:100
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:10-1:500
Application Notes
This Dnmt1 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry on paraffin-embedded sections and Western Blot, where a band can be seen at approx. 190 kDa. NOTE: This antibody does not neutralize the enzymatic activity of its corresponding methyltransferase.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
190 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 3 Reviews rated 4.3
using
NB100-264 in the following applications:

Publications
Read Publications using
NB100-264 in the following applications:

  • 2 publications
  • IHC
    2 publications
  • 1 publication
  • WB
    11 publications

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
No Preservative
Purity
Unpurified

Alternate Names for DNMT1 Antibody

  • AIM
  • CXXC9
  • CXXC9CXXC finger protein 9
  • CXXC-type zinc finger protein 9
  • DNA (cytosine-5-)-methyltransferase 1
  • DNA (cytosine-5)-methyltransferase 1
  • DNA methyltransferase 1
  • DNA methyltransferase HsaI
  • DNA MTase HsaI
  • DNMT
  • DNMT1
  • EC 2.1.1.37
  • FLJ16293
  • m.HsaI
  • MCMT
  • MCMTMGC104992

Background

Member of C5-methyltransferase family and the most abundant DNA methyltransferase in mammalian cells, Dnmt1 (DNA methyltransferase 1) is a key methylation enzyme implicated in maintaining methylation during DNA replication/repair which has capability of methylating CpG residues with preference to hemimethylated DNA and de novo methylation during somatic cell development/differentiation. Dnmt1 interacts with CSNK1D, HDAC1, PCNA, MBD2, MBD3, DNMT3A, DNMT3B, PRC2/EED-EZH2 complex, UBC9, BAZ2A/TIP5, UHRF1, USP7 etc., and forms complexes with DMAP1, HDAC2, E2F1, BB1, HDAC1 and SUV39H1. In cell division, Dnmt1 associates with DNA replication sites in S phase maintaining the methylation pattern in newly synthesized strand, that is essential for epigenetic inheritance. Moreover, it associates with chromatin during G2 as well as M phases to maintain DNA methylation independently of replication, and is reduced to non detectable levels at the G0 phase with dramatic induction upon entry into S-phase. Dnmt1 mediates transcriptional repression by direct binding to HDAC2 and in association with DNMT3B/via the recruitment of CTCFL/BORIS, it involves in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 as well as H3K9. Dnmt1 is responsible for maintaining methylation patterns established in development and defects in DNMT1 are the cause of hereditary sensory neuropathy type 1E (HSN1E).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DNMT1 Antibody (NB100-264)(22)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-P, WB.


Filter By Application
ICC/IF
(2)
IHC
(2)
IHC-P
(1)
WB
(11)
All Applications
Filter By Species
Human
(13)
Mouse
(5)
All Species
Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using NB100-264 Applications Species
Gu H, Gao J, Guo W et al. The expression of DNA methyltransferases3A is specifically downregulated in chorionic villi of early embryo growth arrest cases. Mol Med Rep. 2017 Jul 01 [PMID: 28560437] (IHC-P, Human) IHC-P Human
Chang CC, Pirozzi G, Wen SH et al. Multiple Structural and Epigenetic Defects in the Human Leukocyte Antigen class I Antigen Presentation Pathway in a Recurrent Metastatic Melanoma Following Immunotherapy. J. Biol. Chem. 2015 Sep 17 [PMID: 26381407] (WB, Human) WB Human
Vispe S, Deroide A, Davoine E et al. Consequences of combining siRNA-mediated DNA methyltransferase 1 depletion with 5-aza-2'-deoxycytidine in human leukemic KG1 cells. Oncotarget 2015 Jun 20 [PMID: 25948775] (WB, Human) WB Human
Cui Y, Irudayaraj J. Dissecting the behavior and function of MBD3 in DNA methylation homeostasis by single-molecule spectroscopy and microscopy. Nucleic Acids Res. 2015 Mar 31 [PMID: 25753672] (ICC/IF, Human) ICC/IF Human
Wong M, Gertz B, Chestnut BA, Martin LJ. Mitochondrial DNMT3A and DNA methylation in skeletal muscle and CNS of transgenic mouse models of ALS. Front Cell Neurosci 2013 [PMID: 24399935] (Mouse) Mouse
Lai AY, Mav D, Shah R et al. DNA methylation profiling in human B cells reveals immune regulatory elements and epigenetic plasticity at Alu elements during B-cell activation. Genome Res 2013 Dec [PMID: 24013550] (IHC, Human) IHC Human
Gros C, Chauvigne L, Poulet A et al. Development of a universal radioactive DNA methyltransferase inhibition test for high-throughput screening and mechanistic studies. Nucleic Acids Res. 2013 Aug 25 [PMID: 23980028] (WB, Human) WB Human
Chang VH, Chu PY, Peng SL et al. Kruppel-like factor 10 expression as a prognostic indicator for pancreatic adenocarcinoma. Am J Pathol. 2012 Aug [PMID: 22688058] (WB, Human) WB Human
Yin LJ, Zhang Y, Lv PP et al. Insufficient maintenance DNA methylation is associated with abnormal embryonic development BMC Med 2012 Mar 13 [PMID: 22413869] (WB, IHC, Human, Mouse) WB, IHC Human, Mouse
Selvakumar T, Gjidoda A, Hovde SL, Henry RW. Regulation of Human RNA Polymerase III Transcription by DNMT1 and DNMT3a DNA Methyltransferases J Biol Chem 2012 Mar 2 [PMID: 22219193] (WB, Human) WB Human
Show All 22 Publications.

Reviews for DNMT1 Antibody (NB100-264) (3) 4.33

Average Rating: 4.3
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Human.
We have 3 reviews tested in 1 application: WB.

Reviews using NB100-264:
Filter by Applications
WB
(3)
All Applications
Filter by Species
Human
(3)
All Species
Images Ratings Applications Species Date Details
Western Blot DNMT1 NB100-264
Enlarge
reviewed by:
Anonymous
WB Human 09/20/2017
View

Summary

ApplicationWestern Blot
Sample Tested fibroblasts
SpeciesHuman
Lotc-3

Comments

Comments4-12% Bis-Tris gel, 30ug of whole cell lysate.
 
reviewed by:
Anonymous
WB Human 11/30/2011
View

Summary

ApplicationWestern Blot
Sample TestedHuman
SpeciesHuman
CommentsGood!

Blocking

Blocking DetailsBlocking Buffer: 5% BSA in 0.1% PBST

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab Dilution Ratio: 1:5000

Details

Detection NotesDetection Method: ECL Plus

Comments

CommentsGood!
 
reviewed by:
Anonymous
WB Human 11/08/2011
View

Summary

ApplicationWestern Blot
Sample TestedHuman
SpeciesHuman
CommentsOk

Blocking

Blocking DetailsBlocking Buffer: 5% BSA in 2% dry milk

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab: goat anti-mouse IgG HRP

Details

Detection NotesDetection Method: ECL

Comments

CommentsOk

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DNMT1 Antibody (NB100-264). (Showing 1 - 2 of 2 FAQs).

  1. Does this Dnmt1 antibody (NB100-264) work on paraformaldehyde fixed mouse brain tissue? Does the antibody also work in Western blot with mouse brain?
    • Product NB100-264 has not been tested for use with mouse, or in IHC. We have confirmed the effectiveness of this antibody in Western blot, but only on human samples. Due to the fact that the antibody has been validated to stain cells in an immunocytochemistry application, it is highly likely to stain tissues in IHC as well. If you are interested in testing this antibody for use in either mouse and/or IHC we would encourage you to apply for our Innovators Reward Program. Under the terms of this program, you will be eligible for a full credit in exchange for your data, in the form of an online review, regardless of positive or negative results. Please contact us at innovators@novusbio.com for additional information. Our lab only recommends testing on a new species if the homology is approximately 85% or above to give you the best chance at a successful assay. The homology between the human protein and mouse protein is reasonably high within the first 125 amino acids on the N-terminus, making this one a great candidate for further mouse testing.
  2. Do you have a recommended blocking agent for use with Dnmt1 Antibody (NB100-264)?
    • For Western blot, we recommend blocking with 5% milk and 1-3% BSA. The following is a link where you can find support by application including our general protocols.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for DNMT1 Antibody (NB100-264)

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Blogs on DNMT1.

The role of DNMT3B in the co-incidence of methyltransferase and tumor suppressor expression in malignancies
Epigenetics is the process of heritable change in gene activity despite alteration of the hosts DNA sequence, essentially causing a change in a phenotype without a change in the genotype of a host. To change the gene sequence without interfering w...  Read full blog post.

The role of DNMT3A in development
Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence.  Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation.  Gene silencing in DNA ...  Read full blog post.

Controlling Epigenetic Signaling with Dnmt1 and Dnmt3b
Dnmt1 belongs to the C5-methyltransferase family that repairs cytosines in dsDNA using a nucleophilic attack mechanism. Dnmt1 is the most abundant mammalian DNA methyltransferase. It is the key methylation maintenance enzyme for both DNA replication/r...  Read full blog post.

PCNA Antibodies: Marking Cell Proliferation & DNA Replication
Proliferating Cell Nuclear Antigen (PCNA), also known as the polymerase delta auxiliary protein, is a nuclear protein essential for DNA replication as well as DNA excision and mismatch repair pathways. It has a large role in cell cycle regulation and...  Read full blog post.

DNMT's: An Overview of 3 DNA Methyltransferases
DNA methyltransferases catalyze the transfer of the methyl group from S-andenosyl methionine (SAM) to DNA. Such methylation has wide ranging function in the cell, including organismal development and cell differentiation. In cancer, abnormal hypermeth...  Read full blog post.

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Recent Reviews

4.3
5
1
4
2
3
0
2
0
1
0

Anonymous
09/20/2017
Application: WB
Species: Human

 
Anonymous
11/30/2011
Application: WB
Species: Human

 
Anonymous
11/08/2011
Application: WB
Species: Human

Bioinformatics

Gene Symbol DNMT1
Entrez
Uniprot