DNER Antibody

Western blot shows lysates of human brain (cerebellum) tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human DNER Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3646) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for DNER at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

DNER Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human DNER
  Arg29-His637
  Accession # Q8NFT8
• Specificity: Detects human DNER in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 30% cross-reactivity with recombinant mouse DNER is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DNER
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DNER Antibody

• bet
• delta and Notch-like epidermal growth factor-related receptor
• delta/notch-like EGF repeat containing
• delta-notch-like EGF repeat-containing transmembrane
• DNER
• H_NH0150O02.1
• UNQ26
• WUGSC:H_NH0150O02.1

Background

DNER, also known as brain EGF repeat containing transmembrane protein (BET), is a neuron-specific type I transmembrane notch ligand that is expressed on membranes of dendrites and cell bodies but not axons. It contains ten extracellular EGF-like domains and one follistatin-like domain (between the 9^th and 10^th EGF-like domains). DNER mediates neuron-glia interactions and is required for the functional and morphological maturation of the cerebellum. The extracellular domains of human and mouse DNER share 89% amino acid sequence homology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DNER Antibody (AF3646)(1)

Publication using AF3646

• C Ballester-, TM Conlon, Z Ertüz, FR Greiffo, M Irmler, SE Verleden, J Beckers, IE Fernandez, O Eickelberg, AÖ Yildirim The Notch ligand DNER regulates macrophage IFN? release in chronic obstructive pulmonary disease EBioMedicine, 2019-05-04;0(0):. 2019-05-04 [PMID: 31060902] (IHC-P, Human)

Review for DNER Antibody (AF3646) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: YONGJIE LAI IF Human 01/13/2015

Summary

• Application: Immunofluorescence
• Sample Tested: HEK 293 cells
• Species: Human

Bioinformatics

• Gene Symbol: DNER
• Uniprot:
  • Q8NFT8()