DNCIC1 Antibody (70.1)

Product Discontinued

DNCIC1 Antibody (70.1) Summary

• Immunogen: Cytoplasmic full length native protein (purified from chick brain) (Chicken).
• Localization: Cytoplasmic and Axonemal Forms
• Specificity: This identifies the cytoplasmic dynein intermediate chains (triplet of approximately 70 kD) of all vertebrates tested, in immunoblotting with dynein enriched preparations and with crude cell extracts.
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Gene: DYNC1I1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Dot Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:2000
• Application Notes: WB: Use at a dilution of 1/2000. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Cross-reacts with Human, Rat, Chicken, Fish, Frog, Indian Muntjac, Xenopus laevis and Marsupials. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.09% Sodium Azide
• Purity: Unpurified

Alternate Names for DNCIC1 Antibody (70.1)

• cytoplasmic dynein 1 intermediate chain 1
• Cytoplasmic dynein intermediate chain 1
• DNCI1cytoplasmic, intermediate polypeptide 1
• DNCIC1DH IC-1
• Dynein intermediate chain 1, cytosolic
• dynein, cytoplasmic 1, intermediate chain 1

Background

Eukaryotic cells rely on actin and microtubule-based protein "motors" to generate intracellular movements.4 These protein "motors" contain specialized domains that hydrolyse ATP to produce force and movement along a cytoskeletal polymer (actin in the case of myosin family and microtubules in the case of the kinesin family and dyneins). The minus-end-directed, microtubule motor, dynein ATPase is one of the most widely studied microtubule-associated energy transducing enzymes. It constitutes the outer and inner arms on the doublet tubules of sperm flagellar axonemes, where it generates the sliding between doublets that underlies flagellar beating. Dynein has also been implicated in cytoplasmic motile functions, including chromosomal movement, retrograde organelle and axonal transport, the endocytic pathway, and the organization of the Golgi apparatus. In all cell types, dynein has the same basic structures and is composed of two or three distinct heavy chains (approximately 450 kDa), three intermediate chains (70-125 kDa), and at least four light chains (15-25 kDa).5

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DYNC1I1
• Entrez:
  • 1780(Human)
  • 29564(Rat)