DNASE1 Antibody

• Reactivity: Bv
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: LYOPH

DNASE1 Antibody Summary

• Description: Purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against stated buffer.
• Immunogen: Deoxyribonuclease A [Bovine Pancreas]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DNASE1
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This antibody has been tested by western blot and is suitable to be assayed against 1.0 ug of Deoxyribonuclease A [Bovine Pancreas] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:1,500 of the reconstitution concentration is suggested for this product.

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Concentration: LYOPH
• Purity: Multi-step
• Reconstitution Instructions: Reconstitute with 100 ul deionized water (or equivalent).

Alternate Names for DNASE1 Antibody

• deoxyribonuclease IFLJ38093
• deoxyribonuclease-1
• DKFZp686H0155
• DNase I
• DNase I, lysosomal
• DNL1FLJ44902
• Dornase alfa
• DRNI
• EC 3.1.21.1
• human urine deoxyribonuclease I

Background

DNASE1, also known as Deoxyribonuclease 1, is an endonuclease found in the zymogen granules of the nuclear envelope. Responsible for DNA fragmentation and cell death during apoptosis, DNASE1 binds to G-actin and blocks actin polymerization. Mutations in DNASE1 cause systemic lupus erythematosus (SLE), an autoimmune disease causing inflammation and tissue damage. DNASE1 is also known to treat symptoms of cystic fibrosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DNASE1