DNase II Antibody - BSA Free

Immunohistochemistry: DNase II Antibody [NBP1-76971] - Staining of DNase II in human spleen tissue with DNase II antibody at 5 ug/ml.

Immunocytochemistry/ Immunofluorescence: DNase II Antibody - BSA Free [NBP1-76971] - Immunofluorescence of DNase II in human spleen tissue with DNase II antibody at 5 ug/ml. Green: DNase II antibody Red: Phylloidin staining Blue: DAPI staining

Immunohistochemistry: DNase II Antibody - BSA Free [NBP1-76971] - Immunohistochemistry of DNaseII in human spleen tissue with DNaseII antibody at 5 ug/mL.

Western Blot: DNase II Antibody - BSA Free [NBP1-76971] - Western blot analysis of DNase II in human spleen tissue lysate in the absence (lane 1) or presence (lane 2) of blocking peptide with DNase antibody II at 1:500 dilution.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

DNase II Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 14 amino acid peptide near the carboxy terminus of human DNase II. The immunogen is located within the last 50 amino acids of DNase II. Amino Acid Squence: CNGMARKPSRAYKI
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DNASE2
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 5 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 0.5 ug/ml
• Application Notes: An approximate 40 kDa band can be detected, which represents the pro-enzyme of DNase II.
• Theoretical MW: ~40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  DNase II Peptide (NBP1-76971PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for DNase II Antibody - BSA Free

• Acid DNase
• Deoxyribonuclease II alpha
• deoxyribonuclease II, lysosomal
• deoxyribonuclease-2-alpha
• DNase II alpha
• DNase II, lysosomal
• DNASE2A
• DNL
• DNL2
• EC 3.1.22.1
• Lysosomal DNase II
• R31240_2

Background

Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins including AIF and Acinus. DNase II causes both chromatin condensation and DNA fragmentation. The genes encoding human, porcine, and murine DNase II have been cloned. The DNase II gene encodes a 40 kDa proenzyme. The mature enzyme consists of two non-identical subunits, the 32 kDa (alpha) and 12 kDa (beta) chains, generated by proteolytic processing. Overexpression of DNase II induces chromatin condensation. DNase II is ubiquitously expressed in human tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DNase II Antibody (NBP1-76971)(1)

Publication using NBP1-76971

• Volcic M, Sparrer KMJ, Koepke L et al. Vpu modulates DNA repair to suppress innate sensing and hyper-integration of HIV-1 Nat Microbiol 2020-07-20 [PMID: 32690953] (WB, Human)

Bioinformatics

• Gene Symbol: DNASE2
• Entrez:
  • 1777(Human)
• Uniprot:
  • O00115(Human)