DNA Polymerase delta, catalytic subunit Antibody - BSA Free

Immunoprecipitation:DNA Polymerase delta, catalytic subunit AntibodyNBP3-30106] -Detection of human PolD1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PolD1 antibody NBP3-30106 (lot NBP3-30106-2) used for IP at 6 µg per reaction. PolD1 was also immunoprecipitated by a previous lot of this antibody (lot NBP3-30106-1). For blotting immunoprecipitated PolD1, NBP3-30106 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

DNA Polymerase delta, catalytic subunit Antibody - BSA Free Summary

• Immunogen: Between 930 and 980
• Assay Type: [Assay Type]
• Sample Volume: [Sample Volume]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: POLD1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-citrate/phosphate buffer, pH 7 to 8
• Preservative: 0.09% Sodium Azide
• Purity: Affinity purified

Alternate Names for DNA Polymerase delta, catalytic subunit Antibody - BSA Free

• CDC2 homolog
• CDC2
• DNA polymerase delta catalytic subunit
• DNA polymerase subunit delta p125
• DNA-directed polymerase delta 1
• EC 2.7.7.7
• POLD
• polymerase (DNA directed), delta 1, catalytic subunit (125kD)
• polymerase (DNA directed), delta 1, catalytic subunit 125kDa

Background

DNA polymerase d (delta) functions in DNA replication and DNA repair. It also possesses 3' to 5' exonuclease activity. In leading strand synthesis it requires the processivity factor, PCNA. It is also possibly involved in the completion of DNA synthesis during Okazaki fragment elongation following the initiation of synthesis by the DNA polymerase a/primase complex

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POLD1