DNA Polymerase delta, catalytic subunit Antibody (S05-3H1)

Western Blot: DNA Polymerase delta, catalytic subunit Antibody (S05-3H1) [NBP3-19932] - Western blot detection of DNA Polymerase delta, catalytic subunit in K562, C6, 3T3, Hela cell lysates using NBP3-19932 (1:1000 diluted). Predicted band size: 124kDa. Observed band size: 124kDa.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: S05-3H1
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

DNA Polymerase delta, catalytic subunit Antibody (S05-3H1) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human DNA Polymerase delta, catalytic subunit (Uniprot # P28340)
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: POLD1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1/1000-1/5000
• Theoretical MW: 124 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Affinity purified

Alternate Names for DNA Polymerase delta, catalytic subunit Antibody (S05-3H1)

• CDC2 homolog
• CDC2
• DNA polymerase delta catalytic subunit
• DNA polymerase subunit delta p125
• DNA-directed polymerase delta 1
• EC 2.7.7.7
• POLD
• polymerase (DNA directed), delta 1, catalytic subunit (125kD)
• polymerase (DNA directed), delta 1, catalytic subunit 125kDa

Background

DNA polymerase d (delta) functions in DNA replication and DNA repair. It also possesses 3' to 5' exonuclease activity. In leading strand synthesis it requires the processivity factor, PCNA. It is also possibly involved in the completion of DNA synthesis during Okazaki fragment elongation following the initiation of synthesis by the DNA polymerase a/primase complex

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POLD1