DNA Polymerase delta, catalytic subunit Antibody (607)

Images

 
Western Blot: DNA Polymerase delta, catalytic subunit Antibody (607) [NB100-476] - Sample (30 ug of whole cell lysate) A: BCL-1 5% SDS PAGE; antibody diluted at 1:1000.
Western Blot: DNA Polymerase delta, catalytic subunit Antibody (607) [NB100-476] - HeLa whole cell extract.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clone
607
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

DNA Polymerase delta, catalytic subunit Antibody (607) Summary

Immunogen
Recombinant protein encoding amino acids 1-140 of the large subunit of human DNA polymerase d.
Localization
Nuclear
Isotype
IgG
Clonality
Monoclonal
Host
Mouse
Gene
POLD1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
Application Notes
WB: Detects an approx. 130 kDa protein. Predicted MW is 130 kDa.
Positive Control
HeLa Lysate (NB800-PC1)
DNA Polymerase delta, catalytic subunit Lysate (NBL1-14567)
Publications
Read Publication using NB100-476.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for DNA Polymerase delta, catalytic subunit Antibody (607)

  • CDC2 homolog
  • CDC2
  • DNA polymerase delta catalytic subunit
  • DNA polymerase subunit delta p125
  • DNA-directed polymerase delta 1
  • EC 2.7.7.7
  • POLD
  • polymerase (DNA directed), delta 1, catalytic subunit (125kD)
  • polymerase (DNA directed), delta 1, catalytic subunit 125kDa

Background

DNA polymerase d (delta) functions in DNA replication and DNA repair. It also possesses 3' to 5' exonuclease activity. In leading strand synthesis it requires the processivity factor, PCNA. It is also possibly involved in the completion of DNA synthesis during Okazaki fragment elongation following the initiation of synthesis by the DNA polymerase a/primase complex

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB

Publications for DNA Polymerase delta, catalytic subunit Antibody (NB100-476)(1)

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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol POLD1
Entrez