DNA Polymerase delta, catalytic subunit Antibody (607) - Azide and BSA Free

Western Blot: DNA Polymerase delta, catalytic subunit Antibody (607) [NB100-476] - HeLa whole cell extract.

Western Blot: DNA Polymerase delta, catalytic subunit Antibody (607) [NB100-476] - Sample (30 ug of whole cell lysate) A: BCL-1 5% SDS PAGE; antibody diluted at 1:1000.

Western Blot: DNA Polymerase delta, catalytic subunit Antibody (607) [NB100-476] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DNA Polymerase delta, catalytic subunit antibody [607] (NB100-476) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu
• Applications: WB
• Clone: 607
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

DNA Polymerase delta, catalytic subunit Antibody (607) - Azide and BSA Free Summary

• Immunogen: Recombinant protein encoding amino acids 1-140 of the large subunit of human DNA polymerase d.
• Localization: Nuclear
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: POLD1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 124 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  HeLa Whole Cell Lysate
  DNA Polymerase delta, catalytic subunit Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for DNA Polymerase delta, catalytic subunit Antibody (607) - Azide and BSA Free

• CDC2 homolog
• CDC2
• DNA polymerase delta catalytic subunit
• DNA polymerase subunit delta p125
• DNA-directed polymerase delta 1
• EC 2.7.7.7
• POLD
• polymerase (DNA directed), delta 1, catalytic subunit (125kD)
• polymerase (DNA directed), delta 1, catalytic subunit 125kDa

Background

DNA polymerase d (delta) functions in DNA replication and DNA repair. It also possesses 3' to 5' exonuclease activity. In leading strand synthesis it requires the processivity factor, PCNA. It is also possibly involved in the completion of DNA synthesis during Okazaki fragment elongation following the initiation of synthesis by the DNA polymerase a/primase complex

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DNA Polymerase delta, catalytic subunit Antibody (NB100-476)(1)

Publication using NB100-476

• Kundu, C et al. Adenomatous polyposis coli-mediated hypersensitivity of mouse embryonic fibroblast cell lines to methylmethane sulfonate treatment: implication of base excision repair pathways. Carcinogenesis;28: 2089-2095. [PMID: 17522063]

Bioinformatics

• Gene Symbol: POLD1
• Entrez:
  • 5424(Human)