DNA Polymerase delta, catalytic subunit Antibody (4B6I3)

Western Blot: DNA Polymerase delta, catalytic subunit Antibody (4B6I3) [NBP3-16358] - Western blot analysis of extracts of various cell lines, using DNA Polymerase delta, catalytic subunit Rabbit mAb (NBP3-16358) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 4B6I3
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

DNA Polymerase delta, catalytic subunit Antibody (4B6I3) Summary

• Description: Novus Biologicals Rabbit DNA Polymerase delta, catalytic subunit Antibody (4B6I3) (NBP3-16358) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1000-1107 of human DNA Polymerase delta, catalytic subunit (P28340). GGLLAFAKRRNCCIGCRTVLSHQGAVCEFCQPRESELYQKEVSHLNALEERFSRLWTQCQRCQGSLHEDVICTSRDCPIFYMRKKVRKDLEDQEQLLRRFGPPGPEAW
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: POLD1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for DNA Polymerase delta, catalytic subunit Antibody (4B6I3)

• CDC2 homolog
• CDC2
• DNA polymerase delta catalytic subunit
• DNA polymerase subunit delta p125
• DNA-directed polymerase delta 1
• EC 2.7.7.7
• POLD
• polymerase (DNA directed), delta 1, catalytic subunit (125kD)
• polymerase (DNA directed), delta 1, catalytic subunit 125kDa

Background

DNA polymerase d (delta) functions in DNA replication and DNA repair. It also possesses 3' to 5' exonuclease activity. In leading strand synthesis it requires the processivity factor, PCNA. It is also possibly involved in the completion of DNA synthesis during Okazaki fragment elongation following the initiation of synthesis by the DNA polymerase a/primase complex

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POLD1