DLL4: Novus Biologicals

AF1389

DLL4 Antibody [Unconjugated]

(1 Review)

100 μg

AF1389

HAF017). A specific band was detected for DLL4 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse DLL4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1389) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF017). A specific band was detected for DLL4 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse DLL4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1389) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for DLL4 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

DLL4 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Mouse DLL4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1389) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <a class=

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to developing vasculature. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

DLL4 was detected in immersion fixed paraffin-embedded sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse DLL4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1389) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to developing vasculature. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

MPDZ promotes Notch signaling activity.(A) HUVECs were either transduced with lentivirus expressing GFP (sh-ctrl) or with lentivirus expressing shRNA against MPDZ (sh-MPDZ). Expression level of Notch target genes HEY1, HEY2 and HES1 were analyzed by qPCR 48 hr after transduction. Data are presented as mean ±SD. n ≥ 3; *, p<0.05; **, p<0.01; ***, p<0.001 unpaired Student’s t-test. (B) Cardiac endothelial cells were isolated from Mpdzfl/fl and Mpdz delta EC mice by magnetic beads bound with CD31 antibodies. Expression levels of Notch target genes Hey1 and Hey2 were analyzed by qPCR. Data are presented as mean ±SD. n = 3; *, p<0.05; ***, p<0.001 unpaired Student’s t-test. (C) HUVECs were either transduced with lentivirus expressing GFP (sh-ctrl) or with lentivirus expressing shRNA against MPDZ (sh-MPDZ). Expression levels of DLL1 and DLL4 were analyzed by immunoblotting 48 hr after transduction. beta -actin served as loading control. Data are presented as mean ±SD. n ≥ 3; n.s., not significant. (D) HUVECs were either transduced with adenovirus expressing GFP (ctrl) or with adenovirus expressing MPDZ. Expression levels of DLL1 and DLL4 were analyzed by immunoblotting 48 hr after transduction. beta -actin served as loading control. Data are presented as mean ±SD. n ≥ 3; n.s., not significant. (E) Lung endothelial cells were isolated from Mpdzfl/fl and Mpdz delta EC mice by CD31 magnetic beads. Protein amounts of Dll1 and Dll4 were analyzed by immunoblotting. beta -actin served as loading control. Data are presented as mean ±SD. n = 3; n.s., not significant.10.7554/eLife.32860.007Figure 2—source data 1.Source data of qantitative PCR analysis related to Figure 2A and B.Source data of qantitative PCR analysis related to Figure 2A and B. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/29620522), licensed under a CC-BY license. Not internally tested by R&D Systems.

Mpdz does not affect cell cell junction assembly.(A, B) HUVECs were either transduced with lentivirus expressing GFP (sh-ctrl) or with lentivirus expressing shRNA against MPDZ (sh-MPDZ). Cells were cultured under sparse conditions (A) or confluent conditions (B). After PFA fixation cells were stained for DLL1 and Nectin-2 or DLL4 and Nectin-2 and counterstained with DAPI. Images were acquired with the confocal microscope LSM 700. Arrow indicates co-localization of DLL1/4 with Nectin-2 at the cell membrane. Arrow head indicates diminished co-localization at the cell membrane. Scale bar: 10 µm. (C) HUVECs were either transfected with control siRNA (si-ctrl) or with siRNA against Nectin-2 (si-Nectin-2). After PFA fixation cells were stained for DLL1 and Nectin-2 or DLL4 and Nectin-2. Images were acquired with the confocal microscope LSM 700. Arrow indicates localization of DLL1/4 at the cell membrane.Scale bar: 10 µm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/29620522), licensed under a CC-BY license. Not internally tested by R&D Systems.

Endothelial changes after pericyte depletion. a–f Maximum intensity projection of confocal images from control and DTRiPC P6 retinas stained for IB4 (red) in combination with VEGF-A a, VEGFR2 b, VEGFR3 c, Tie2 d, Esm1 e, and Dll4 f (all in white), as indicated. Note local increase of VEGFR2, VEGFR3, and Esm1 (arrowheads in b, c, e) but not Tie2 or VEGF-A at the edge of the vessel plexus. Dll4 expression in DTRiPC sprouts is increased in some regions (arrowheads) but absent in others (arrows). Scale bar, 100 µm. g–j Quantitation of VEGF-A immunosignals area and intensity g, signal intensity for VEGFR2 h and VEGFR3 i and proportion of Esm1+ area with respect to vascular area j in the P6 control and DTRiPC angiogenic front. Error bars, s.e.m. p-values, Student’s t-test. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/29146905), licensed under a CC-BY license. Not internally tested by R&D Systems.

Targeting of Fbxw7 induced the upregulation of Dll4 expression.Anti-Dll4 and Isolectin B4 (IsolB4)-stained retinal whole-mounts of Fbxw7iECKO and littermate control retinas, as indicated (A, B). Arrowheads in (B) mark Dll4+ peripheral sprouts at the edge of the growing plexus, arrows indicate upregulated Dll4 in Fbxw7iECKO retinal capillaries. Quantitative analysis (with Volocity 5; n = 3 for each group) of image data confirmed elevated Dll4 levels (number of pixels) in the mutant endothelium (C). Likewise, quantitative RT-PCR analysis showed reduced Fbxw7 expression but upregulated Dll4 transcript levels in P6 Fbxw7iECKO lungs (D). Expression of the Cdh5 gene was used for normalization. Error bars indicate SEM. P values are indicated as ** (<0.001) and * (p<0.05). Scale bars are 100 µm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/22848434), licensed under a CC-BY license. Not internally tested by R&D Systems.

DLL4 expressed from the Dll1 locus rescues DLL1 loss-of-function in the retina.(A)Dll1 null mutant retinas show epithelial disruption with formation of polarised rosettes in which the apical markers N-Cadherin (NCad, a) and ZO-1 (ZO1, b) are abnormally present at the central lumen. Ectopic proliferating progenitors, labelled with PHH3 (b, arrowheads), are located close to the apical lumen of these rosettes. (B) In contrast, the neuroepithelium of homozygous Dll1Dll1ki and Dll1Dll4ki embryos is correctly organised without rosettes, and N-Cadherin shows the normal apical localisation close to the retinal pigmented epithelium (a,b). Mitotic progenitors (PHH3+) are only detected at the apical region of the neuroepithelium (a,b arrowheads). A normal stratification of CHX10+ progenitors and P27+ differentiating neurons is also observed (c,d). (C, D) E13.5 homozygous Dll1Dll1ki and Dll1Dll4ki retinas show no significant difference in the number of ISL1+ RGCs (C) and CRABP+ amacrine cells (D). Cells immunopositive for Islet-1 and Crabp were counted and related to the total number of cells in the retina (DAPI+). Percentages are shown as mean ± SEM; ns, not significant. (E) Expression of DLL4 in homozygous Dll1Dll1ki (a,c) and in homozygous Dll1Dll4ki (b,d) E13.5 retinas as detected by an anti-DLL4 antibody. (c) and (d) are magnifications of (a) and (b), respectively. Endogenous plus transgenic DLL4 is expressed in more cells in Dll1Dll4ki/Dll4ki as compared to endogenous DLL4 expression in Dll1Dll1ki/Dll1ki while signal strength is similar. Scale bars are 50 μm in (A, B) and 100 μm in (E). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26114479), licensed under a CC-BY license. Not internally tested by R&D Systems.

Dll1 and Dll4 are expressed in distinct patterns in the mice gastrointestinal epithelium.Immunohistochemistry of Dll1 and Dll4 were performed using mice small intestinal (A, B) and colonic tissues (D, E). Within the small intestinal epithelium, staining by anti-Dll1 antibody showed positive cells exclusively within the crypt (A, B), whereas staining by anti-Dll4 antibody showed positive cells both in the crypt and in the villi. Quantification of Dll1+ve and Dll4+ve intestinal epithelial cells (IECs) showed that Dll1+ve IECs are predominantly found within the crypt, whereas Dll4+ve IECs are found mostly in the villi (C). In the colonic epithelium, staining by anti-Dll1 antibody showed positive cells exclusively within the lower part of the crypt (D, E), whereas staining by anti-Dll4 antibody showed positive cells both in the lower- and upper part of the crypt, including the surface epithelium. Quantification of Dll1+ve and Dll4+ve IECs showed that Dll1+ve IECs are found exclusively within the lower half of the crypt, whereas Dll4+ve IECs are found both in the lower and upper region of the crypt at a comparable frequency (F). Negative staining of non-immunized isotype antibodies (Control Ab) confirmed the specific staining of Dll1 (A) and Dll4 (D). Scale bar represents 20 µm. Quantitative data are shown as mean ∓ SD of triplicate experiments (n = 3). ∗ indicates P < 0.05 as determined by Welch’s t-test. N.S. indicates not significant. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/24860699), licensed under a CC-BY license. Not internally tested by R&D Systems.

Dll1+ve or Dll4+ve IECs constitute the intestinal stem cell niche.Double immunostaining of Dll1 or Dll4 with a stem-cell niche cell marker, Lysozyme or c-kit, was performed in the mice small intestine and in the colon, respectively. (A) Immunostaining of Dll4 (green) with Lysozyme (red) shows that Dll4 is expressed in Paneth cells of the small intestine (yellow arrowhead). Scale bar represents 20 µm. (B) Double immunostaining of Dll1 (green) and c-kit (red) shows that Dll1+ve colonic IECs mostly co-express c-kit (yellow arrowhead). Also, double immunostaining of Dll4 (green) and c-kit (red) showed that a distinct population of Dll4+ve cells co-express c-kit (yellow arrowhead). Scale bar represents 20 µm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/24860699), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF1389U-100UG
Alexa Fluor 405	AF1389V-100UG
Alexa Fluor 532	AF1389X-100UG
Alexa Fluor 594	AF1389T-100UG
Alexa Fluor 647	AF1389R-100UG
Alexa Fluor 700	AF1389N-100UG
Alexa Fluor 750	AF1389S-100UG
Alexa Fluor Plus 405	AF1389AFP405-100UG
Alexa Fluor Plus 488	AF1389AFP488-100UG
Alexa Fluor Plus 555	AF1389AFP555-100UG
Alexa Fluor Plus 594	AF1389AFP594-100UG
Alexa Fluor Plus 647	AF1389AFP647-100UG
Alexa Fluor Plus 680	AF1389AFP680-100UG
Biotin	BAF1389
CoraFluor 1	AF1389CL1
DyLight 350	AF1389D
DyLight 405	AF1389E
DyLight 488	AF1389K
DyLight 550	AF1389L
DyLight 594	AF1389M
DyLight 650	AF1389W
DyLight 680	AF1389Y
DyLight 755	AF1389Z
FITC	AF1389F
HRP	AF1389H
Janelia Fluor 525	AF1389JF525
Janelia Fluor 549	AF1389I
Janelia Fluor 646	AF1389J
Janelia Fluor 669	AF1389JF669
PE	AF1389P
PerCP	AF1389C
mFluor Violet 450 SE	AF1389MFV450
mFluor Violet 500 SE	AF1389MFV500
mFluor Violet 610 SE	AF1389MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF1389-SP

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NB600-892

DLL4 Antibody - BSA Free

(1 Review)

(22 Publications)

0.1 mg

NB600-892

Western Blot: DLL4 Antibody [NB600-892] - Western Blot of DLL4 antibody. Lane 1: mouse pancreatic tissue. Load: 20 ug per lane.Primary antibody: DLL4 antibody at 1:500 for overnight at 4C.Secondary antibody: HRP-labeled Goat anti-Rabbit IgG secondary antibody at 1:5,000 for 1 hour at RT. A chemiluminescence system was used for signal detection using a 3 min exposure time.Block: 5% Goat Serum overnight at 4C.Predicted/Observed size: 74 kDa, 70 kDa for Delta 4. Other band(s): none.

ELISA: DLL4 Antibody [NB600-892] - ELISA results of purified DLL4 antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Peroxidase Substrate.

Affinity Purified anti-Delta-4 antibody was used at 20 ug/ml to detect Delta-4 in a variety of tissues including colon, liver, skeletal muscle, ovary, pancreas, prostate, testes, thymus, tonsil and uterus. In contrast to reported findings, no staining was observed in vascular tissue. This image shows Delta-4 staining of human ovary. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.

Expression of NICD in human platelets.(A) Immunoblot showing expression of NICD in DLL-4 (15 ug/ml for 10 min)-treated platelets in absence or presence of either DAPT (10 uM) or DBZ (10 uM) or vehicle. (B) Corresponding densitometric analysis of NICD normalised with beta -actin (n=6). (C, D and E) Immunoblot of NICD expression in either stored or A23187 (1 uM)-treated platelets under conditions as indicated. Data are represented as mean +/- SEM of at least three individual experiments and analyzed by RM one-way ANOVA with Dunnett’s multiple comparisons test.Figure 2—source data 1.Excel sheet shows numerical data of Figure 2.Figure 2—source data 2.Unedited and labelled blots of Figure 2.Figure 2—source data 3.Unedited and unlabelled blots of Figure 2.Excel sheet shows numerical data of Figure 2.Unedited and labelled blots of Figure 2.Unedited and unlabelled blots of Figure 2. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36190110), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB600-892SS

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1506-D4

Recombinant Human DLL4 His-tag Protein

(22 Publications)

50 μg

1506-D4

355-BM) induced alkaline phosphatase activity in the C3H10T1/2 mouse embryonic fibroblast cell line. The ED₅₀ for this effect is 150-600 ng/mL." class="big_lightbox">

355-BM) induced alkaline phosphatase activity in the C3H10T1/2 mouse embryonic fibroblast cell line. The ED₅₀ for this effect is 150-600 ng/mL." alt="Recombinant Human DLL4 (Catalog # 1506-D4) enhances Recombinant Human/Mouse/Rat BMP-2 (Catalog # 355-BM) induced alkaline phosphatase activity in the C3H10T1/2 mouse embryonic fibroblast cell line. The ED₅₀ for this effect is 150-600 ng/mL." class="big_thumb" />

1 µg/lane of Recombinant Human DLL4 was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing major bands at 60-70 kDa.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	1506-D4-050/CF

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MAB1389

DLL4 Antibody (207822) [Unconjugated]

(10 Publications)

500 μg

MAB1389

CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to developing cardiomyocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox">

DLL4 was detected in immersion fixed frozen sections of mouse embryonic heart using Rat Anti-Human/Mouse DLL4 Monoclonal Antibody (Catalog # MAB1389) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to developing cardiomyocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." alt="DLL4 was detected in immersion fixed frozen sections of mouse embryonic heart using Rat Anti-Human/Mouse DLL4 Monoclonal Antibody (Catalog # MAB1389) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to developing cardiomyocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

mDia1is essential for CCN1-induced Cdc42 activation in tip cell formation.(A) HUVECs were treated with PBS (Control) or CCN1 (10 ng/mL) for 24 hr, and total RNA was used for the detection of DIAPH1 (mDia1) mRNA expression by qRT-PCR. *p<0.001 vs. Control. (B) After transfection with DIAPH1 WT full length and DIAPH1 double-negative mutant plasmids, HUVECs were treated with CCN1 and immunostained with anti-vinculin antibody (red) and phalloidin (green) to visualise filopodia. Scale bar = 10 µm. (C, D) HUVECs transfected with DIAPH1 WT full length and DIAPH1 double-negative mutant plasmids were treated with CCN1 (10 ng/mL) for 30 min, and active Cdc42 was assessed by western blotting analysis (C) and visualised by p-N-Wasp (D). Fold changes were noted under each protein band. (E) After HUVECs were transfected with DIAPH1 WT full length and DIAPH1 DN3 mutant plasmid, starved cells for 16 hr were treated with CCN1 10 ng/ml for 1 hr and detected YAP/TAZ by IF. Scale bar = 100 µm.10.7554/eLife.46012.012Figure 4—source data 1.Source data for Figure 4C.Source data for Figure 4C. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31429823), licensed under a CC-BY license. Not internally tested by R&D Systems.

CCN1 stimulates tip cell activity in ECs. (G) Western blotting analysis of VEGFR2, DLL4, and SOX17 protein levels in the same samples as used in F. beta -actin was used as an internal control. Fold increase was indicated as a number below each band. (H) Luciferase assays in HUVECs transfected with DLL4-Luc reporter vector and treated with CCN1 or VEGF (10 ng/mL) for 24 hr. Luciferase activity was normalised to Renilla luminescence. *p<0.001 vs. Control.10.7554/eLife.46012.005Figure 2—source data 1.Source data for Figure 2G.Source data for Figure 2G. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31429823), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

,

Applications: ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB1389U-100UG
Alexa Fluor 405	FAB1389V-100UG
Alexa Fluor 532	FAB1389X-100UG
Alexa Fluor 594	FAB1389T-100UG
Alexa Fluor 647	FAB1389R-100UG
Alexa Fluor 700	FAB1389N-100UG
Alexa Fluor 750	FAB1389S-100UG
Alexa Fluor Plus 405	FAB1389AFP405-100UG
Alexa Fluor Plus 488	FAB1389AFP488-100UG
Alexa Fluor Plus 555	FAB1389AFP555-100UG
Alexa Fluor Plus 594	FAB1389AFP594-100UG
Alexa Fluor Plus 647	FAB1389AFP647-100UG
Alexa Fluor Plus 680	FAB1389AFP680-100UG
Biotin	FAB1389B
CoraFluor 1	FAB1389CL1
DyLight 350	FAB1389D
DyLight 405	FAB1389E
DyLight 488	FAB1389K
DyLight 550	FAB1389L
DyLight 594	FAB1389M
DyLight 650	FAB1389W
DyLight 680	FAB1389Y
DyLight 755	FAB1389Z
FITC	FAB1389F
HRP	FAB1389H
Janelia Fluor 525	FAB1389JF525
Janelia Fluor 549	FAB1389I
Janelia Fluor 646	FAB1389J
Janelia Fluor 669	FAB1389JF669
PE	FAB1389P
PerCP	FAB1389C
mFluor Violet 450 SE	FAB1389MFV450
mFluor Violet 500 SE	FAB1389MFV500
mFluor Violet 610 SE	FAB1389MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB1389-SP

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MAB1506

DLL4 Antibody (447506)

(3 Publications)

100 μg

MAB1506

MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0105B)." class="big_lightbox">

MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0105B)." alt="HUVEC human umbilical vein endothelial cells were stained with Rat Anti-Human DLL4 Monoclonal Antibody (Catalog # MAB1506, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0105B)." class="big_thumb" />

NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

DLL4 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Rat Anti-Human DLL4 Monoclonal Antibody (Catalog # MAB1506) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # <a class=

NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB1506U-100UG
Alexa Fluor 405	FAB1506V-100UG
Alexa Fluor 488	FAB1506G-100UG
Alexa Fluor 532	FAB1506X
Alexa Fluor 594	FAB1506T-100UG
Alexa Fluor 647	FAB1506R-100UG
Alexa Fluor 700	FAB1506N-100UG
Alexa Fluor 750	FAB1506S-100UG
Alexa Fluor Plus 405	FAB1506AFP405-100UG
Alexa Fluor Plus 488	FAB1506AFP488-100UG
Alexa Fluor Plus 555	FAB1506AFP555-100UG
Alexa Fluor Plus 594	FAB1506AFP594-100UG
Alexa Fluor Plus 647	FAB1506AFP647-100UG
Alexa Fluor Plus 680	FAB1506AFP680-100UG
Allophycocyanin/Cy7	FAB1506APCCY7
Biotin	FAB1506B
CoraFluor 1	FAB1506CL1
DyLight 350	FAB1506D
DyLight 405	FAB1506E
DyLight 488	FAB1506K
DyLight 550	FAB1506L
DyLight 594	FAB1506M
DyLight 650	FAB1506W
DyLight 680	FAB1506Y
DyLight 755	FAB1506Z
FITC	FAB1506F
HRP	FAB1506H
Janelia Fluor 525	FAB1506JF525
Janelia Fluor 549	FAB1506I
Janelia Fluor 646	FAB1506J
Janelia Fluor 669	FAB1506JF669
PE/Atto594	FAB1506PEATT594
PE/Cy5.5	FAB1506PECY55
PE/Cy7	FAB1506PECY7
PerCP	FAB1506C
Phycoerythrin	FAB1506P
mFluor Violet 450 SE	FAB1506MFV450
mFluor Violet 500 SE	FAB1506MFV500
mFluor Violet 610 SE	FAB1506MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB1506P
Carrier Free	MAB1506-SP
Carrier Free	FAB1506V-100UG
Carrier Free	FAB1506U-100UG
Carrier Free	FAB1506T-100UG
Carrier Free	FAB1506S-100UG
Carrier Free	FAB1506N-100UG
Carrier Free	FAB1506G-100UG
Carrier Free	FAB1506R-100UG

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NBP2-27407

DLL4 Antibody

0.1 mg

NBP2-27407

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-6596A

Alt Catalog# IMG-6596A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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MAB13891

DLL4 Antibody (207811) [Unconjugated]

500 μg

MAB13891

1389-D4) was serially diluted and captured by Rat Anti-Mouse DLL4 Monoclonal Antibody (Catalog # MAB13891) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse DLL4 Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_lightbox">

Recombinant Mouse DLL4 His-tag (Catalog # <a class=

1389-D4) was serially diluted and captured by Rat Anti-Mouse DLL4 Monoclonal Antibody (Catalog # MAB13891) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse DLL4 Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" alt="Recombinant Mouse DLL4 His-tag (Catalog # 1389-D4) was serially diluted and captured by Rat Anti-Mouse DLL4 Monoclonal Antibody (Catalog # MAB13891) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse DLL4 Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB13891U-100UG
Alexa Fluor 405	FAB13891V-100UG
Alexa Fluor 488	FAB13891G-100UG
Alexa Fluor 532	FAB13891X-100UG
Alexa Fluor 594	FAB13891T-100UG
Alexa Fluor 647	FAB13891R-100UG
Alexa Fluor 700	FAB13891N-100UG
Alexa Fluor 750	FAB13891S-100UG
Alexa Fluor Plus 405	FAB13891AFP405-100UG
Alexa Fluor Plus 488	FAB13891AFP488-100UG
Alexa Fluor Plus 555	FAB13891AFP555-100UG
Alexa Fluor Plus 594	FAB13891AFP594-100UG
Alexa Fluor Plus 647	FAB13891AFP647-100UG
Alexa Fluor Plus 680	FAB13891AFP680-100UG
Allophycocyanin	FAB13891A
Biotin	FAB13891B
CoraFluor 1	FAB13891CL1
DyLight 350	FAB13891D
DyLight 405	FAB13891E
DyLight 488	FAB13891K
DyLight 550	FAB13891L
DyLight 594	FAB13891M
DyLight 650	FAB13891W
DyLight 680	FAB13891Y
DyLight 755	FAB13891Z
FITC	FAB13891F
HRP	FAB13891H
Janelia Fluor 525	FAB13891JF525
Janelia Fluor 549	FAB13891I
Janelia Fluor 646	FAB13891J
Janelia Fluor 669	FAB13891JF669
PE	FAB13891P
PerCP	FAB13891C
mFluor Violet 450 SE	FAB13891MFV450
mFluor Violet 500 SE	FAB13891MFV500
mFluor Violet 610 SE	FAB13891MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB13891-SP

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DY1389

Mouse DLL4 DuoSet ELISA, 15 Plate

1 Kit (for 15 Plates)

DY1389

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

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NBP1-86413

DLL4 Antibody - BSA Free

(1 Publication)

0.1 ml

NBP1-86413

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP3-28485

DLL4 Antibody - Humanized, IgG2SA, (Research Grade navicixizumab Biosimilar) - Low Endotoxin, Azide and BSA Free

100 μg

NBP3-28485

Immobilized human VEGF165 His at 2 ug/mL can bind DLL4 Antibody (navicixizumab) - Humanized, IgG2SA, EC50=0.01191 ug/mL.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

CHO Monoclonal

Host: CHO Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-28485AF350
Alexa Fluor 405	NBP3-28485AF405
Alexa Fluor 488	NBP3-28485AF488
Alexa Fluor 532	NBP3-28485AF532
Alexa Fluor 594	NBP3-28485AF594
Alexa Fluor 647	NBP3-28485AF647
Alexa Fluor 700	NBP3-28485AF700
Alexa Fluor 750	NBP3-28485AF750
Alexa Fluor Plus 405	NBP3-28485AFP405
Alexa Fluor Plus 488	NBP3-28485AFP488
Alexa Fluor Plus 555	NBP3-28485AFP555
Alexa Fluor Plus 594	NBP3-28485AFP594
Alexa Fluor Plus 647	NBP3-28485AFP647
Alexa Fluor Plus 680	NBP3-28485AFP680
Allophycocyanin	NBP3-28485APC
Biotin	NBP3-28485B
CoraFluor 1	NBP3-28485CL1
DyLight 350	NBP3-28485UV
DyLight 405	NBP3-28485V
DyLight 488	NBP3-28485G
DyLight 550	NBP3-28485R
DyLight 594	NBP3-28485DL594
DyLight 650	NBP3-28485C
DyLight 680	NBP3-28485FR
DyLight 755	NBP3-28485IR
FITC	NBP3-28485F
HRP	NBP3-28485H
Janelia Fluor 525	NBP3-28485JF525
Janelia Fluor 549	NBP3-28485JF549
Janelia Fluor 646	NBP3-28485JF646
Janelia Fluor 669	NBP3-28485JF669
PE	NBP3-28485PE
PerCP	NBP3-28485PCP
mFluor Violet 450 SE	NBP3-28485MFV450
mFluor Violet 500 SE	NBP3-28485MFV500
mFluor Violet 610 SE	NBP3-28485MFV610

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NBP3-28648

DLL4 Antibody - (Research Grade enoticumab Biosimilar) - Low Endotoxin, Azide and BSA Free

100 μg

NBP3-28648

Immobilized human DLL4 His at 2 ug/mL can bind DLL4 Antibody (enoticumab), EC50=0.003918 ug/mL.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

CHO Monoclonal

Host: CHO Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-28648AF350
Alexa Fluor 405	NBP3-28648AF405
Alexa Fluor 488	NBP3-28648AF488
Alexa Fluor 532	NBP3-28648AF532
Alexa Fluor 594	NBP3-28648AF594
Alexa Fluor 647	NBP3-28648AF647
Alexa Fluor 700	NBP3-28648AF700
Alexa Fluor 750	NBP3-28648AF750
Alexa Fluor Plus 405	NBP3-28648AFP405
Alexa Fluor Plus 488	NBP3-28648AFP488
Alexa Fluor Plus 555	NBP3-28648AFP555
Alexa Fluor Plus 594	NBP3-28648AFP594
Alexa Fluor Plus 647	NBP3-28648AFP647
Alexa Fluor Plus 680	NBP3-28648AFP680
Allophycocyanin	NBP3-28648APC
Biotin	NBP3-28648B
CoraFluor 1	NBP3-28648CL1
DyLight 350	NBP3-28648UV
DyLight 405	NBP3-28648V
DyLight 488	NBP3-28648G
DyLight 550	NBP3-28648R
DyLight 594	NBP3-28648DL594
DyLight 650	NBP3-28648C
DyLight 680	NBP3-28648FR
DyLight 755	NBP3-28648IR
FITC	NBP3-28648F
HRP	NBP3-28648H
Janelia Fluor 525	NBP3-28648JF525
Janelia Fluor 549	NBP3-28648JF549
Janelia Fluor 646	NBP3-28648JF646
Janelia Fluor 669	NBP3-28648JF669
PE	NBP3-28648PE
PerCP	NBP3-28648PCP
mFluor Violet 450 SE	NBP3-28648MFV450
mFluor Violet 500 SE	NBP3-28648MFV500
mFluor Violet 610 SE	NBP3-28648MFV610

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NBP2-62229

DLL4 Antibody (MHD4-46) - Low Endotoxin - Low Endotoxin, Azide and BSA Free

0.1 mg

NBP2-62229

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-62229AF350
Alexa Fluor 405	NBP2-62229AF405
Alexa Fluor 488	NBP2-62229AF488
Alexa Fluor 532	NBP2-62229AF532
Alexa Fluor 594	NBP2-62229AF594
Alexa Fluor 647	NBP2-62229AF647
Alexa Fluor 700	NBP2-62229AF700
Alexa Fluor 750	NBP2-62229AF750
Alexa Fluor Plus 405	NBP2-62229AFP405
Alexa Fluor Plus 488	NBP2-62229AFP488
Alexa Fluor Plus 555	NBP2-62229AFP555
Alexa Fluor Plus 594	NBP2-62229AFP594
Alexa Fluor Plus 647	NBP2-62229AFP647
Alexa Fluor Plus 680	NBP2-62229AFP680
Allophycocyanin	NBP2-62229APC
Allophycocyanin/Cy7	NBP2-62229APCCY7
Biotin	NBP2-62229B
CoraFluor 1	NBP2-62229CL1
DyLight 350	NBP2-62229UV
DyLight 405	NBP2-62229V
DyLight 488	NBP2-62229G
DyLight 550	NBP2-62229R
DyLight 594	NBP2-62229DL594
DyLight 650	NBP2-62229C
DyLight 680	NBP2-62229FR
DyLight 755	NBP2-62229IR
FITC	NBP2-62229F
HRP	NBP2-62229H
Janelia Fluor 525	NBP2-62229JF525
Janelia Fluor 549	NBP2-62229JF549
Janelia Fluor 646	NBP2-62229JF646
Janelia Fluor 669	NBP2-62229JF669
PE	NBP2-62229PE
PE/Atto594	NBP2-62229PEATT594
PE/Cy5.5	NBP2-62229PECY55
PE/Cy7	NBP2-62229PECY7
PerCP	NBP2-62229PCP
mFluor Violet 450 SE	NBP2-62229MFV450
mFluor Violet 500 SE	NBP2-62229MFV500
mFluor Violet 610 SE	NBP2-62229MFV610

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Preservative

Protein Or Peptide

Has Sample Size

Promotional Item

Species Glossary

DLL4 Products By Gene Name

DLL4 Antibody [Unconjugated]

DLL4 Antibody - BSA Free

Recombinant Human DLL4 His-tag Protein

DLL4 Antibody (207822) [Unconjugated]

DLL4 Antibody (447506)

DLL4 Antibody

DLL4 Antibody (207811) [Unconjugated]

Mouse DLL4 DuoSet ELISA, 15 Plate

DLL4 Antibody - BSA Free

DLL4 Antibody - Humanized, IgG2SA, (Research Grade navicixizumab Biosimilar) - Low Endotoxin, Azide and BSA Free

DLL4 Antibody - (Research Grade enoticumab Biosimilar) - Low Endotoxin, Azide and BSA Free

DLL4 Antibody (MHD4-46) - Low Endotoxin - Low Endotoxin, Azide and BSA Free