Pref-1/DLK1/FA1 Antibody (211309)

Images

 
Pref‑1/DLK1/FA1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Pref‑1/DLK1/FA1 Monoclonal Antibody (Catalog # MAB1144) at 10 µg/mL for 3 hours at room ...read more
HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Pref‑1/DLK1/FA1 Monoclonal Antibody (Catalog # MAB1144, filled histogram) or isotype control antibody (Catalog # MAB0041, open ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready, ICC/IF
Clone
211309
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Pref-1/DLK1/FA1 Antibody (211309) Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Pref-1 long isoform
Ala24-Pro297 (with Arg248Pro and Lys295Ser substitutions)
Accession # P80370
Specificity
Detects human Pref‑1/DLK‑1/FA1 in direct ELISAs and Western blots. Shows approximately 50% cross-reactivity with recombinant mouse Pref‑1.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
DLK1
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
MAB1144 in the following applications:

Publications
Read Publications using
MAB1144 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Pref-1/DLK1/FA1 Antibody (211309)

  • delta-like 1 homolog (Drosophila)
  • DLK
  • DLK1
  • DLK-1
  • FA1
  • FA1fetal antigen 1
  • pG2
  • pG2delta-like homolog (Drosophila)
  • preadipocyte factor 1
  • Pref1
  • Pref-1
  • protein delta homolog 1
  • secredeltin
  • ZOG

Background

Pref-1 (Preadipocyte factor 1; also DLK-1 and FA1) is a 58-65 kDa member of the Notch/Serrata/Delta family of proteins.  It is expressed in prechondrocytes and preadipocytes and appears to block progenitor cell differentiation into mature cell lineages.  Mature human Pref-1 is a 360 amino acid (aa) type I transmembrane N- and O-linked glycoprotein.  It contains a 280 aa extracellular region (aa 24-303), a 24 aa transmembrane segment (aa 304-327), and a 56 aa cytoplasmic domain (aa 328-383).  The extracellular region contains six EGF-like domains, and undergoes proteolytic cleavage to generate a bioactive 50 kDa fragment, plus three 25‑31 kDa fragments that show no activity.  There are multiple potential splice variants.  One shows a deletion of aa 229-301, a second possesses a six aa substitution for aa 1‑52, a third shows a deletion of aa 210-277, while a fourth contains a six aa substitution for aa 207-383.  Over aa 24-297, human Pref-1 shares 82% aa identity with mouse Pref-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Pref-1/DLK1/FA1 Antibody (MAB1144)(4)

Review for Pref-1/DLK1/FA1 Antibody (MAB1144) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB1144:
Filter by Applications
Flow
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Flow Cytometry Pref-1/DLK1/FA1 MAB1144
Enlarge
5
reviewed by:
Ming Lu
Flow Human 01/09/2018
View

Summary

ApplicationFlow Cytometry
Sample TestedNIH3T3 cells overxpressing human DLK1
SpeciesHuman
Lot1YH0415091

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Pref-1/DLK1/FA1 Antibody (MAB1144) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB1144U
Alexa Fluor 405 FAB1144V
Alexa Fluor 488 FAB1144G
Alexa Fluor 488 FAB1144G
Alexa Fluor 594 FAB1144T
Alexa Fluor 647 FAB1144R
Alexa Fluor 700 FAB1144N
Alexa Fluor 750 FAB1144S
Allophycocyanin FAB1144A
Allophycocyanin FAB1144A

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Bioinformatics Tool for Pref-1/DLK1/FA1 Antibody (MAB1144)

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Visit Tool

Diseases for Pref-1/DLK1/FA1 Antibody (MAB1144)

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Blogs on Pref-1/DLK1/FA1

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Ming Lu
01/09/2018
Application: Flow
Species: Human

Bioinformatics

Gene Symbol DLK1
Uniprot