Pref-1/DLK1/FA1 Antibody (211309)

Pref‑1/DLK1/FA1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Pref‑1/DLK1/FA1 Monoclonal Antibody (Catalog # MAB1144) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Pref‑1/DLK1/FA1 Monoclonal Antibody (Catalog # MAB1144, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready, ICC/IF
• Clone: 211309
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Pref-1/DLK1/FA1 Antibody (211309) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Pref-1 long isoform
  Ala24-Pro297 (with Arg248Pro and Lys295Ser substitutions)
  Accession # P80370
• Specificity: Detects human Pref‑1/DLK‑1/FA1 in direct ELISAs and Western blots. Shows approximately 50% cross-reactivity with recombinant mouse Pref‑1.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: DLK1
• Purity: Protein A or G purified from hybridoma culture supernatant
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Pref-1/DLK1/FA1 Antibody (211309)

• delta-like 1 homolog (Drosophila)
• DLK
• DLK1
• DLK-1
• FA1
• FA1fetal antigen 1
• pG2
• pG2delta-like homolog (Drosophila)
• preadipocyte factor 1
• Pref1
• Pref-1
• protein delta homolog 1
• secredeltin
• ZOG

Background

Pref-1 (Preadipocyte factor 1; also DLK-1 and FA1) is a 58-65 kDa member of the Notch/Serrata/Delta family of proteins.  It is expressed in prechondrocytes and preadipocytes and appears to block progenitor cell differentiation into mature cell lineages.  Mature human Pref-1 is a 360 amino acid (aa) type I transmembrane N- and O-linked glycoprotein.  It contains a 280 aa extracellular region (aa 24-303), a 24 aa transmembrane segment (aa 304-327), and a 56 aa cytoplasmic domain (aa 328-383).  The extracellular region contains six EGF-like domains, and undergoes proteolytic cleavage to generate a bioactive 50 kDa fragment, plus three 25‑31 kDa fragments that show no activity.  There are multiple potential splice variants.  One shows a deletion of aa 229-301, a second possesses a six aa substitution for aa 1‑52, a third shows a deletion of aa 210-277, while a fourth contains a six aa substitution for aa 207-383.  Over aa 24-297, human Pref-1 shares 82% aa identity with mouse Pref-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Pref-1/DLK1/FA1 Antibody (MAB1144)(4)

Publications using MAB1144

• JC Gerlach, RL Thompson, B Gridelli, E Schmelzer Effects of Delta-Like Noncanonical Notch Ligand 1 Expression of Human Fetal Liver Hepatoblasts on Hematopoietic Progenitors Stem Cells Int, 2019;2019(0):7916275. 2019 [PMID: 31011334] (IHC, Human)
• Begum A, Lin Q, Yu C, Kim Y, Yun Z Interaction of delta-like 1 homolog (Drosophila) with prohibitins and its impact on tumor cell clonogenicity. Mol Cancer Res, 2014;12(1):155-64. 2014 [PMID: 24249679] (ICC, Human)
• Kim Y, Lin Q, Zelterman D, Yun Z Hypoxia-regulated delta-like 1 homologue enhances cancer cell stemness and tumorigenicity. Cancer Res., 2009;69(24):9271-80. 2009 [PMID: 19934310] (ICC, Human)
• Chung S, Lapoint K, Martinez K, Kennedy A, Boysen Sandberg M, McIntosh MK Preadipocytes mediate lipopolysaccharide-induced inflammation and insulin resistance in primary cultures of newly differentiated human adipocytes. Endocrinology, 2006;147(11):5340-51. 2006 [PMID: 16873530] (Western Blot, Human)

Review for Pref-1/DLK1/FA1 Antibody (MAB1144) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Ming Lu Flow Human 01/09/2018

Summary

• Application: Flow Cytometry
• Sample Tested: NIH3T3 cells overxpressing human DLK1
• Species: Human
• Lot: 1YH0415091

Bioinformatics

• Gene Symbol: DLK1
• Uniprot:
  • P80370()