Dkk-1 Antibody (141119)

• Reactivity: Hu
• Applications: WB
• Clone: 141119
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Dkk-1 Antibody (141119) Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Dkk-1
  Thr32-His266
  Accession # O94907
• Specificity: Detects human Dkk-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows approximately 50% cross‑reactivity with recombinant mouse (rm) Dkk-1 and no cross-reactivity with rmDkk-2, rhDkk-3, or rhDkk-4.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: DKK1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Dkk-1 Antibody (141119)

• dickkopf (Xenopus laevis) homolog 1
• dickkopf homolog 1 (Xenopus laevis)
• dickkopf related protein-1
• Dickkopf-1
• dickkopf-related protein 1
• Dkk1
• Dkk-1
• hDkk-1
• SKdickkopf-1 like

Background

Dkk-1 is a Dickkopf-related protein that inhibits Wnt signaling by binding the Wnt co-receptor LRP5/6. It also binds Kremen-1 and Kremen-2. Dkk-1 is important in embryonic development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Dkk-1 Antibody (MAB1096)(3)

Publications using MAB1096

• Verch T, Chilewski S, Bouaraphan S, Yarovoi H, Yin KC, Chen D, Washabaugh MW Pharmacokinetic immunoassay methods in the presence of soluble target. J. Immunol. Methods, 2010-08-07;361(1):75-81. 2010-08-07 [PMID: 20696169] (ELISA Development, Human)
• Liu CC, Pearson C, Bu G Cooperative folding and ligand-binding properties of LRP6 beta-propeller domains. J. Biol. Chem., 2009-03-31;284(22):15299-307. 2009-03-31 [PMID: 19339249] (Western Blot, Human)
• Giuliani N, Morandi F, Tagliaferri S, Lazzaretti M, Donofrio G, Bonomini S, Sala R, Mangoni M, Rizzoli V Production of Wnt inhibitors by myeloma cells: potential effects on canonical Wnt pathway in the bone microenvironment. Cancer Res., 2007-08-15;67(16):7665-74. 2007-08-15 [PMID: 17702698] (Western Blot, Human)

Bioinformatics

• Gene Symbol: DKK1
• Uniprot:
  • O94907()