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DKFZP434B168 Antibody

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Western Blot: DKFZP434B168 Antibody [NB100-465] - Detection of Human INT7 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-INT7 antibody NB100-465 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Biological Strategies: Western Blot: DKFZP434B168 Antibody [NB100-465] - Detection of Human INT7 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and Jurkat cells prepared using NETN lysis buffer. ...read more
Immunohistochemistry-Paraffin: DKFZP434B168 Antibody [NB100-465] - Human breast carcinoma. Antibody used at a dilution of 1:1000 (1ug/ml).
Western Blot: DKFZP434B168 Antibody [NB100-465] - Nuclear extract (NE) from HeLa cells. Antibody used at 0.1 ug/ml.
Immunohistochemistry-Paraffin: DKFZP434B168 Antibody [NB100-465] - FFPE section of human breast carcinoma Affinity purified rabbit anti-INT7 used at a dilution of 1:250. Detection: DAB staining using ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

      

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DKFZP434B168 Antibody Summary

Immunogen
The immunogen this antibody was made to, maps to a region between residues 850-900 of human DKFZP434B168 using the TrEMBL entry Q9NVH2 (GeneID 25896).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
INTS7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:10000-1:25000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:500-1:2000
Application Notes
Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
Publications
Read Publication using NB100-465.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DKFZP434B168 Antibody

  • C1orf73
  • chromosome 1 open reading frame 73
  • DKFZp434B168
  • INT7
  • integrator complex subunit 7

Background

INTS7 is a subunit of the Integrator complex, which associates with the C-terminal domain of RNA polymerase II large subunit (POLR2A; MIM 180660) and mediates 3-prime end processing of small nuclear RNAs U1 (RNU1; MIM 180680) and U2 (RNU2; MIM 180690) (Baillat et al., 2005 [PubMed 16239144]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry: CDT2 Antibody [NB100-40840] - Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-DTL/CDT2 used at a dilution of 1:200 (1ug/ml). Detection: DAB
Western Blot: CDT2 Antibody [NB100-40840] - Detection of Human DTL/CDT2 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti- DTL/CDT2 antibody NB100-40840 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B). DTL/CDT2 was also immunoprecipitated by other rabbit anti- DTL/CDT2 antibodies and NB100-40839, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
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Western Blot: E2F-4 Antibody [NBP1-21374] - Detection of Human E2F4 by Western Blot. Samples: Whole cell lysate from HeLa (5, 15, 50 ug) and 293T (50ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-E2F4 antibody NBP1-21374 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
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DKFZP434B168
Antibody
NB100-465
DKFZP434B168 Antibody
Species: Hu
Applications: WB, IHC, IHC-P

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Bioinformatics

Gene Symbol INTS7
Entrez
Uniprot