![Western Blot: DKFZP434B168 Antibody [NB100-465] - Detection of Human INT7 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-INT7 antibody NB100-465 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/DKFZP434B168-Antibody-Western-Blot-NB100-465-img0008.jpg "Western Blot: DKFZP434B168 Antibody [NB100-465] - Detection of Human INT7 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-INT7 antibody NB100-465 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Description | Novus Biologicals Rabbit DKFZP434B168 Antibody - BSA Free (NB100-465) is a polyclonal antibody validated for use in IHC and WB. Anti-DKFZP434B168 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | The immunogen this antibody was made to, maps to a region between residues 850-900 of human DKFZP434B168 using the TrEMBL entry Q9NVH2 (GeneID 25896). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | INTS7 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NB100-465 | Applications | Species |
|---|---|---|
| Egloff S, Szczepaniak SA, Dienstbier M et al. The integrator complex recognizes a new double mark on the RNA polymerase II carboxyl-terminal domain. J Biol Chem 2010-07-01 [PMID: 20457598] |
Secondary Antibodies |
Isotype Controls |
Research Areas for DKFZP434B168 Antibody (NB100-465)Find related products by research area.
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![Immunohistochemistry-Paraffin: DKFZP434B168 Antibody [NB100-465] - Human breast carcinoma. Antibody used at a dilution of 1:1000 (1ug/ml).](http://images.novusbio.com/fullsize/DKFZP434B168-Antibody-Immunohistochemistry-Paraffin-NB100-465-img0003.jpg "Immunohistochemistry-Paraffin: DKFZP434B168 Antibody [NB100-465] - Human breast carcinoma. Antibody used at a dilution of 1:1000 (1ug/ml).")
![Western Blot: DKFZP434B168 Antibody [NB100-465] - Nuclear extract (NE) from HeLa cells. Antibody used at 0.1 ug/ml.](http://images.novusbio.com/fullsize/DKFZP434B168-Antibody-Western-Blot-NB100-465-img0004.jpg "Western Blot: DKFZP434B168 Antibody [NB100-465] - Nuclear extract (NE) from HeLa cells. Antibody used at 0.1 ug/ml.")
![Immunohistochemistry-Paraffin: DKFZP434B168 Antibody [NB100-465] - FFPE section of human breast carcinoma Affinity purified rabbit anti-INT7 used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit](http://images.novusbio.com/fullsize/DKFZP434B168-Antibody-Immunohistochemistry-Paraffin-NB100-465-img0001.jpg "Immunohistochemistry-Paraffin: DKFZP434B168 Antibody [NB100-465] - FFPE section of human breast carcinoma Affinity purified rabbit anti-INT7 used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit")
![N/A TFPI [HRP]](https://images.novusbio.com/images2/DATA_TFPI_DTFP10_ELISA_841.jpg)
![N/A TFPI [HRP]](https://images.novusbio.com/images2/TFPI_DTFP10_ELISA_190.jpg)
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![N/A BAFF/BLyS/TNFSF13B [HRP]](https://images.novusbio.com/images2/DATA_BAFF_DBLYS0_ELISA_589.jpg)
![N/A Thrombospondin-1 [HRP]](https://images.novusbio.com/images2/Thrombospondin-1_DTSP10_ELISA_199.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")