DISC1 [p Ser710] Antibody

Western Blot: DISC1 [p Ser710] Antibody [NBP3-23278] - Western blot of DISC1 phosphorylation in PC12 cells untreated (lanes 1 & 3) or treated with calyculin A (100 nm) for 30 min (lanes 2 & 4). The blots were probed with NBP3-23277 anti-DISC1 (a.a. 740-753) (lanes 1 & 2) or NBP3-23278 anti-DISC1 (Ser-710) (lanes 3 & 4).

• Reactivity: Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

DISC1 [p Ser710] Antibody Summary

• Description: Stable for at least 1 year at -20°C.
• Immunogen: Phospho-DISC1 (Ser-710) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Ser-710 in mouse DISC1. This peptide sequence is well conserved in rat DISC1 (Ser-703), but has five amino acid differences with the conserved site in human DISC1 (Ser-713). [UniProt# Q811T9]
• Modification: p Ser710
• Specificity: The antibody detects a 100 kDa protein corresponding to the molecular mass of DISC1 on SDS-PAGE immunoblots of PC12 cells treated with calyculin A. This reactivity is not observed after lambda phosphatase treatment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DISC1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 100 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS, 1 mg/ml BSA and 50% glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Alternate Names for DISC1 [p Ser710] Antibody

• C1orf136
• DISC1
• disrupted in schizophrenia 1 protein
• disrupted in schizophrenia 1
• FLJ13381
• FLJ21640
• FLJ25311
• FLJ41105
• KIAA0457
• SCZD9

Background

The Disrupted In Schizophrenia gene locus DISC is associated with patients afflicted with schizophrenia as a result of chromosomal translocations. DISC-1 encodes a large protein predicted to contain a globular N-terminal domain and a helical C-terminal domain, both of which have the potential to form interactions with other proteins. DISC-1 interacts with proteins involved in the centrosome and cytoskeletal system, including MIPT3, MAP1A and NUDEL; proteins which localize receptors to membranes, including alpha-actinin 2 an beta 4-spectrin; and proteins which transduce signals from membrane receptors, including ATF-4 and ATF-5. Therefore, DISC-1 is thought to be involved in intracellular transport, neurite architecture and/or neuronal migration, all of which are thought to be pathogenic in the schizophrenic brain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DISC1