DIO3 Antibody


Western Blot: DIO3 Antibody [NB110-96414] - Detection of Dio3 in rat placenta

Product Details

Reactivity Hu, Mu, Rt, HaSpecies Glossary
Applications WB, ICC/IF, IHC
1 mg/ml

DIO3 Antibody Summary

Synthetic peptide made to an internal portion of rat DIO3 (within residues 1-100). [Swiss-Prot# P49897]
Cell membrane; Single-pass type II membrane protein
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS, 30% glycerol
0.1% Sodium Azide
1 mg/ml
Immunogen affinity purified


Application Notes
This DIO3 antibody is useful for Immunocytochemistry/Immunofluorescence (PMID: 21828183) and Western blot, where a band is seen ~31 kDa

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
31 kDa
Control Peptide
DIO3 Peptide (NB110-96414PEP)
Read Publications using
NB110-96414 in the following applications:

  • 1 publication
  • IHC
    2 publications
  • WB
    4 publications

Reactivity Notes

Human, rat, mouse and hamster. Human reactivity was confirmed in PMID 22723689.

Alternate Names for DIO3 Antibody

  • 5DIII
  • D3
  • deiodinase, iodothyronine, type III
  • EC 1.97.1
  • EC
  • ITDI3
  • placental type
  • thyroxine deiodinase type III (selenoprotein)
  • Type 3 DI
  • type 3 iodothyronine selenodeiodinase
  • type III iodothyronine deiodinase
  • type-III 5' deiodinase
  • Type-III 5'-deiodinase


DIO3 (type III iodothyronine deiodinase), an iodothyronine deiodinase family member, is expressed in placenta and several fetal tissues in the plasma/endosomal membranes as single-pass type II membrane protein, and it catalyzes the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into RT3 (3,3',5'-triiodothyronine) and of T3 (3,5,3'-triiodothyronine) into T2 (3,3'-diiodothyronine), both RT3 and T2 being inactive metabolites thus preventing premature exposure of developing fetus to adult levels of thyroid hormones. DIO3 controls circulating thyroid hormone levels throughout gestation by controlling thyroid hormone inactivation during embryological development. DIO3 is homodimeric and may undergo minor heretodimerization with DIO1 or DIO2, homo- or heterodimer state being critical for its catalytic activity. Its activity has been identified in only a limited number of postnatal tissues, i.e. brain, skin, and pregnant uterus, whereas it is abundantly present in several fetal tissues. Furthermore, DIO3 re-expression has been shown in certain pathologies that include cancer, cardiac hypertrophy, myocardial infarction, chronic inflammation, and critical illness.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DIO3 Antibody (NB110-96414)(6)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Hamster.

We have publications tested in 3 applications: ICC/IF, IHC, WB.

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Product General Protocols

View specific protocols for DIO3 Antibody (NB110-96414): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DIO3 Antibody (NB110-96414). (Showing 1 - 1 of 1 FAQs).

  1. I am looking for a Dio3 peptide that I can inject in vivo to produce a biological effect. Can you provide information or confirm whether the Dio3 peptide you have would be effective as a biological agent in vivo?
    • I have pulled up information on the Dio3 peptides and recombinant proteins that we have available. Unfortunately I would not expect any of them to be biologically active and capable of producing a response when injected in vivo. The two peptides we carry are simply intended to be used for negative control competition assays to block signal of the primary and determine what is specific and non-specific as far as signal is concerned. The other two recombinant proteins are ones we distribute for a Taiwanese company called Abnova and they should not be biologically active based on the cell free wheat germ system employed to synthesize them. We will typically list our active proteins with an indication of such on our datasheet if it has been tested and usually the application of functional listed.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB110-96414AF405
Alexa Fluor (R) 488 Labeled NB110-96414AF488
Alexa Fluor (R) 647 Labeled NB110-96414AF647
Alexa Fluor (R) 700 Labeled NB110-96414AF700
Biotin Labeled NB110-96414B
DyLight 405 Labeled NB110-96414V
DyLight 488 Labeled NB110-96414G
DyLight 550 Labeled NB110-96414R
DyLight 650 Labeled NB110-96414C
FITC Labeled NB110-96414F
HRP Labeled NB110-96414H

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Contact Information

Product PDFs


Gene Symbol DIO3

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