Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody

Western Blot: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NB300-916] - Staining of Human Cerebellum (A), Kidney (B) and Testes (C) lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NB300-916] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic, nuclear and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Flow Cytometry: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NB300-916] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Western Blot: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NB300-916] - Staining of HepG2 (A) and LNCaP (B) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.

Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NB300-916] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

• Reactivity: Hu, Bv
• Applications: WB, ELISA, Flow, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody Summary

• Description: Novus Biologicals Goat Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody (NB300-916) is a polyclonal antibody validated for use in WB, ELISA, Flow and ICC/IF. Anti-Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence TCCSVLINKKVDS corresponding to C-Terminus according to NP_036269.1, NP_001127917.1.
• Specificity: This antibody is expected to recognize both reported isoforms (NP_036269.1and NP_001127917.1).
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DDAH1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Peptide ELISA Detection limit 1:1000
  • Western Blot 0.1 - 0.3 ug/mL

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody

• DDAH1
• DDAH-1
• DDAHdimethylargininase-1
• DDAHI
• Dimethylargininase-1
• dimethylarginine dimethylaminohydrolase 1FLJ25539
• EC 3.5.3.18
• FLJ21264
• N(G)
• N(G)-dimethylarginine dimethylaminohydrolase 1
• NG, NG-dimethylarginine dimethylaminohydrolase

Background

This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody (NB300-916)(2)

Publications using NB300-916

• Pullamsetti S, Kiss L, Ghofrani HA et al. Increased levels and reduced catabolism of asymmetric and symmetric dimethylarginines in pulmonary hypertension. FASEB J 2005-07-01 [PMID: 15827267]
• Kimoto M, Miyatake S, Sasagawa T et al. Purification, cDNA cloning and expression of human NG,NG-dimethylarginine dimethylaminohydrolase. Eur J Biochem 1998-12-01 [PMID: 9874257]

Bioinformatics

• Gene Symbol: DDAH1
• Entrez:
  • 23576(Human)
• Uniprot:
  • NP_036269.1(Human)