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Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody

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Western Blot: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NB300-916] - Staining of Human Cerebellum (A), Kidney (B) and Testes (C) lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by ...read more
Immunocytochemistry/ Immunofluorescence: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NB300-916] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary ...read more
Flow Cytometry: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NB300-916] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr ...read more
Western Blot: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NB300-916] - Staining of HepG2 (A) and LNCaP (B) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.
Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NB300-916] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by ...read more

Product Details

Summary
Reactivity Hu, BvSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody Summary

Immunogen
Peptide with sequence TCCSVLINKKVDS corresponding to C-Terminus according to NP_036269.1, NP_001127917.1.
Specificity
This antibody is expected to recognize both reported isoforms (NP_036269.1and NP_001127917.1).
Predicted Species
Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DDAH1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Peptide ELISA Detection limit 1:1000
  • Western Blot 0.1 - 0.3 ug/mL
Publications
Read Publications using NB300-916.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody

  • DDAH1
  • DDAH-1
  • DDAHdimethylargininase-1
  • DDAHI
  • Dimethylargininase-1
  • dimethylarginine dimethylaminohydrolase 1FLJ25539
  • EC 3.5.3.18
  • FLJ21264
  • N(G)
  • N(G)-dimethylarginine dimethylaminohydrolase 1
  • NG, NG-dimethylarginine dimethylaminohydrolase

Background

This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody (NB300-916)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DDAH1
Entrez
Uniprot