Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody

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Western Blot: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NB300-916] - Staining of human kideny lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody Summary

Immunogen
Peptide with sequence TCCSVLINKKVDS corresponding to C-Terminus according to NP_036269.1, NP_001127917.1.
Specificity
This antibody is expected to recognize both reported isoforms (NP_036269.1and NP_001127917.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DDAH1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 0.3 ug/ml
  • Peptide ELISA Detection limit 1:1000
Application Notes
WB: Approx 35 kDa band observed in human kidney, human brain (cerebellum) and pancreas lysates (calculated MW of 31.1 kDa according to NP_036269.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (PMID: 15827267).
Publications
Read Publications using NB300-916.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody

  • DDAH1
  • DDAH-1
  • DDAHdimethylargininase-1
  • DDAHI
  • Dimethylargininase-1
  • dimethylarginine dimethylaminohydrolase 1FLJ25539
  • EC 3.5.3.18
  • FLJ21264
  • N(G)
  • N(G)-dimethylarginine dimethylaminohydrolase 1
  • NG, NG-dimethylarginine dimethylaminohydrolase

Background

This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody (NB300-916)(2)

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Bioinformatics

Gene Symbol DDAH1
Entrez
Uniprot