| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85273-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF6530-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-17013-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99138-50ul | |||
![Immunohistochemistry-Paraffin: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NBP1-85273] - Staining in human kidney and skin tissues using anti-DDAH1 antibody. Corresponding DDAH1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Dimethylarginine-Dimethylaminohydrolase-1-DDAH1-Antibody-Immunohistochemistry-Paraffin-NBP1-85273-img0017.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NBP1-85273] - Staining in human kidney and skin tissues using anti-DDAH1 antibody. Corresponding DDAH1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NBP1-85273] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Dimethylarginine-Dimethylaminohydrolase-1-DDAH1-Antibody-Western-Blot-NBP1-85273-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NBP1-85273] - Staining of human cell line U-2 OS shows localization to plasma membrane and cytosol.](http://images.novusbio.com/fullsize/Dimethylarginine-Dimethylaminohydrolase-1-DDAH1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85273-img0018.jpg)
![Western Blot: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NBP1-85273] - Analysis in human cell line SH-SY5Y.](http://images.novusbio.com/fullsize/Dimethylarginine-Dimethylaminohydrolase-1-DDAH1-Antibody-Western-Blot-NBP1-85273-img0007.jpg)
![Immunohistochemistry-Paraffin: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NBP1-85273] - Staining of human kidney shows high expression.](http://images.novusbio.com/fullsize/Dimethylarginine-Dimethylaminohydrolase-1-DDAH1-Antibody-Immunohistochemistry-Paraffin-NBP1-85273-img0013.jpg)
![Immunohistochemistry-Paraffin: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NBP1-85273] - Staining of human skin shows low expression as expected.](http://images.novusbio.com/fullsize/Dimethylarginine-Dimethylaminohydrolase-1-DDAH1-Antibody-Immunohistochemistry-Paraffin-NBP1-85273-img0014.jpg)
![Western Blot: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NB300-916] - Staining of Human Cerebellum (A), Kidney (B) and Testes (C) lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Dimethylarginine-Dimethylaminohydrolase-1-DDAH1-Antibody-Western-Blot-NB300-916-img0002.jpg "Western Blot: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NB300-916] - Staining of Human Cerebellum (A), Kidney (B) and Testes (C) lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NB300-916] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic, nuclear and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/Dimethylarginine-Dimethylaminohydrolase-1-DDAH1-Antibody-Immunocytochemistry-Immunofluorescence-NB300-916-img0003.jpg)
![Flow Cytometry: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NB300-916] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Dimethylarginine-Dimethylaminohydrolase-1-DDAH1-Antibody-Flow-Cytometry-NB300-916-img0005.jpg)
![Western Blot: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NB300-916] - Staining of HepG2 (A) and LNCaP (B) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Dimethylarginine-Dimethylaminohydrolase-1-DDAH1-Antibody-Western-Blot-NB300-916-img0001.jpg)
![Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NB300-916] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/Dimethylarginine-Dimethylaminohydrolase-1-DDAH1-Antibody-NB300-916-img0004.jpg)
![Western Blot: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NBP1-31012] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with DDAH1 antibody C-term diluted at 1:1000.](http://images.novusbio.com/fullsize/Dimethylarginine-Dimethylaminohydrolase-1-DDAH1-Antibody-Western-Blot-NBP1-31012-img0006.jpg "Western Blot: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NBP1-31012] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with DDAH1 antibody C-term diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NBP1-31012] - Analysis of methanol-fixed HCT116, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Dimethylarginine-Dimethylaminohydrolase-1-DDAH1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31012-img0004.jpg)
![Western Blot: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NBP1-31012] - Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Dimethylarginine-Dimethylaminohydrolase-1-DDAH1-Antibody-Western-Blot-NBP1-31012-img0005.jpg)
![Immunohistochemistry-Paraffin: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NBP3-17013] - Staining of human kidney shows high expression.](http://images.novusbio.com/fullsize/Dimethylarginine-Dimethylaminohydrolase-1-DDAH1-Antibody-Immunohistochemistry-Paraffin-NBP3-17013-img0002.jpg "Immunohistochemistry-Paraffin: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NBP3-17013] - Staining of human kidney shows high expression.")
![Immunohistochemistry-Paraffin: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NBP3-17013] - Analysis in human kidney and lymph node tissues using Anti-DDAH1 antibody. Corresponding DDAH1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Dimethylarginine-Dimethylaminohydrolase-1-DDAH1-Antibody-Immunohistochemistry-Paraffin-NBP3-17013-img0001.jpg)
![Immunohistochemistry-Paraffin: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NBP3-17013] - Staining of human lymph node shows low expression as expected.](http://images.novusbio.com/fullsize/Dimethylarginine-Dimethylaminohydrolase-1-DDAH1-Antibody-Immunohistochemistry-Paraffin-NBP3-17013-img0003.jpg)
![Immunohistochemistry-Paraffin: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NBP2-99138] - Immunochemical staining of human Dimethylarginine Dimethylaminohydrolase 1/DDAH1 in human kidney with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Dimethylarginine-Dimethylaminohydrolase-1-DDAH1-Antibody-Immunohistochemistry-Paraffin-NBP2-99138-img0003.jpg "Immunohistochemistry-Paraffin: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NBP2-99138] - Immunochemical staining of human Dimethylarginine Dimethylaminohydrolase 1/DDAH1 in human kidney with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NBP2-99138] - Immunochemical staining of human Dimethylarginine Dimethylaminohydrolase 1/DDAH1 in human hepatoma with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Dimethylarginine-Dimethylaminohydrolase-1-DDAH1-Antibody-Immunohistochemistry-Paraffin-NBP2-99138-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NBP2-99138] - Immunofluorescence staining of Dimethylarginine Dimethylaminohydrolase 1/DDAH1 in HCT116 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99138 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-99138_rabbit-polyclonal-dimethylarginine-dimethylaminohydrolase-1-ddah1-antibody-23520251129365.png)
![Western Blot: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody (1Q5F6) [NBP3-16444] - Analysis of extracts of various cell lines, using Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Rabbit mAb (NBP3-16444) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Dimethylarginine-Dimethylaminohydrolase-1-DDAH1-Antibody-1Q5F6-Western-Blot-NBP3-16444-img0001.jpg "Western Blot: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody (1Q5F6) [NBP3-16444] - Analysis of extracts of various cell lines, using Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Rabbit mAb (NBP3-16444) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody (1Q5F6) [NBP3-16444] - Analysis of extracts of Rat kidney, using Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Rabbit mAb (NBP3-16444) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Dimethylarginine-Dimethylaminohydrolase-1-DDAH1-Antibody-1Q5F6-Western-Blot-NBP3-16444-img0002.jpg)
![Sandwich ELISA: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody (3F7) [H00023576-M01] - Detection limit for recombinant GST tagged DDAH1 is 3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Dimethylarginine-Dimethylaminohydrolase-1-DDAH1-Antibody-3F7-Sandwich-ELISA-H00023576-M01-img0001.jpg "Sandwich ELISA: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody (3F7) [H00023576-M01] - Detection limit for recombinant GST tagged DDAH1 is 3 ng/ml as a capture antibody.")
![Western Blot:[NBP3-29991]-Detection of human DDAH1 by western blot. Samples: Whole cell lysate from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DDAH1 antibody used for WB at 0. 4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-29991_rabbit-dimethylarginine-dimethylaminohydrolase-1-ddah1-pab-2312025128851.jpg "Western Blot:[NBP3-29991]-Detection of human DDAH1 by western blot. Samples: Whole cell lysate from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DDAH1 antibody used for WB at 0. 4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: DDAH1 Overexpression Lysate (Adult Normal) [NBP2-04327] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DDAH1.](http://images.novusbio.com/fullsize/DDAH1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04327-img0001.jpg "Western Blot: DDAH1 Overexpression Lysate (Adult Normal) [NBP2-04327] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DDAH1.")
![Immunoprecipitation:Dimethylarginine Dimethylaminohydrolase 1/DDAH1 AntibodyNBP3-30296] -Detection of human DDAH1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DDAH1 antibody NBP3-30296 (lot NBP3-30296-1) used for IP at 6 µg per reaction. DDAH1 was also immunoprecipitated by rabbit anti-DDAH1 antibody BL21178. For blotting immunoprecipitated DDAH1, NBP3-30296 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-30296_rabbit-dimethylarginine-dimethylaminohydrolase-1-ddah1-pab-3012202420415713.jpg "Immunoprecipitation:Dimethylarginine Dimethylaminohydrolase 1/DDAH1 AntibodyNBP3-30296] -Detection of human DDAH1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DDAH1 antibody NBP3-30296 (lot NBP3-30296-1) used for IP at 6 µg per reaction. DDAH1 was also immunoprecipitated by rabbit anti-DDAH1 antibody BL21178. For blotting immunoprecipitated DDAH1, NBP3-30296 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")