Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody

Dimethylarginine Dimethylaminohydrolase 1/ DDAH1 was detected in immersion fixed paraffin-embedded sections of human brain using Sheep Anti-Human Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6530) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter-stained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody Summary

• Immunogen: E. coli-derived recombinant human Dimethylarginine Dimethylaminohydrolase 1/DDAH1
  Ala2-Ser285
  Accession # O94760
• Specificity: Detects human Dimethylarginine Dimethylaminohydrolase 1/DDAH1 in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: DDAH1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody

• DDAH1
• DDAH-1
• DDAHdimethylargininase-1
• DDAHI
• Dimethylargininase-1
• dimethylarginine dimethylaminohydrolase 1FLJ25539
• EC 3.5.3.18
• FLJ21264
• N(G)
• N(G)-dimethylarginine dimethylaminohydrolase 1
• NG, NG-dimethylarginine dimethylaminohydrolase

Background

Dimethylarginine Dimethylaminohydrolase (DDAH) metabolizes asymmetric dimethyl arginine (ADMA) to L-citrulline and dimethylamine, and N^G-monomethyl arginine (MMA) to L-citrulline and monomethylamine (1). Two members of the DDAH family have been identified in humans. DDAH1 is widely expressed, especially in liver and kidney. DDAH2 predominates in vascular endothelium and expressed selectively in kidney (2). It is also expressed in immune tissues including spleen, thymus, peripheral leukocytes, lymph nodes, and bone marrow. Over 90 % of endogenous ADMA is metabolized by DDAH with the remainder excreted (3). ADMA and MMA are endogenous inhibitors of nitric oxide synthase (NOS). Thus, enzymes of the DDAH family play a key role in vascular function through the turnover of methylated arginine (4). It has been observed that genetic variation in the DDAH1 and DDAH2 genes is significantly associated with serum ADMA levels (5).

1. Tadashi, O. et al. (1989) J. Biol. Chem. 264:10205.
2. Tran, C. T. et al. (2000) Genomics 68:101.
3. Tran, C. T. et al. (2003) Atheroscler. Suppl. 4:33.
4. Leiper, J. et al. (2007) Nat. Med. 13:198.
5. Abhary, S. et al. (2010) PNAS PLoS One. 5:e9462.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DDAH1
• Uniprot:
  • O94760()