Dihydrolipoamide Dehydrogenase/DLD Antibody [HRP]

• Reactivity: Po
• Applications: WB, ELISA, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: HRP
• Concentration: LYOPH

Dihydrolipoamide Dehydrogenase/DLD Antibody [HRP] Summary

• Description: Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
  
  Lipoamide Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Lipoamide Dehydrogenase [Porcine Heart]
• Immunogen: Dihydrolipoamide Dehydrogenase/DLD [Porcine Heart] (Uniprot: P09623)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DLD
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:20000
  • Immunoprecipitation 1:100
  • Western Blot 1:500-1:5000
• Application Notes: This product has been tested by ELISA and Western blot and is assayed against 1.0 ug of Lipoamide Dehydrogenase [Porcine Heart] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggested for this product.
  
  NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
• Readout System:
  • NovaLume Plus Chemiluminescent Reagent LUM-555

Reactivity Notes

Cross reactivity against Dihydrolipoamide Dehydrogenase/DLD from other tissues and species may occur but have not been specifically determined.

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
• Preservative: 0.01% Gentamicin Sulfate
• Concentration: LYOPH
• Purity: Multi-step
• Reconstitution Instructions: Reconstitute with 100 ul deionized water (or equivalent)

Alternate Names for Dihydrolipoamide Dehydrogenase/DLD Antibody [HRP]

• 2-oxo-glutarate complex, branched chain keto acid dehydrogenase complex)
• branched chain keto acid dehydrogenase complex
• Diaphorase
• dihydrolipoamide dehydrogenase (E3 component of pyruvate dehydrogenase complex
• Dihydrolipoamide Dehydrogenase
• dihydrolipoamide dehydrogenasemitochondrial
• DLD
• DLDD
• DLDH
• E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex
• E3
• EC 1.8.1
• EC 1.8.1.4
• GCSL
• Glycine cleavage system L protein
• glycine cleavage system protein L
• LAD
• Lipoamide Dehydrogenase
• lipoamide reductase
• lipoyl dehydrogenase
• PHE3

Background

Lipoamide dehydrogenase or dihydrolipoamide dehydrogenase (DLD) is a component of the glycine cleavage system as well as of the alpha ketoacid dehydrogenase complexes. It is a homodimer. It belongs to the class I pyridine nucleotide disulfide oxidoreductase family. Defects in DLD are a cause of congenital infantile lactic acidosis. Moreover, defects in DLD are the cause of DLD deficiency; which results in extensive metabolic disturbances, including lactic acidemia, Krebs cycle dysfunction, and impaired branched amino acid degradation. DLD deficiency also causes neurological degeneration due to the sensitivity of the central nervous system to defects in oxidative metabolism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DLD
• Uniprot:
  • P09623(Human)