Immunocytochemistry/ Immunofluorescence: Dicer Antibody [NBP1-71691] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were ...read more
Immunohistochemistry-Paraffin: Dicer Antibody [NBP1-71691] - Analysis of Dicer in paraffin embedded human colorectal cancer tissue using anti-Dicer antibody. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: Dicer Antibody [NBP1-71691] - Dicer antibody was tested in HeLa cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry: Dicer Antibody [NBP1-71691] - Staining of Dicer in mouse prostate using DAB with hematoxylin counterstain.
This Dicer antibody is useful for for Immunocytochemistry/Immunofluorescence and Immunohistochemistry-paraffin embedded sections. In ICC/IF, cytoplasmic staining was observed in HeLa cells. In IHC-P, staining was observed in the cytoplasm of mouse prostate. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Read 1 Review rated 4 using NBP1-71691 in the following applications:
Dicer (DICER1 or double-strand-specific ribonuclease mDCR-1) belongs to RNase III family and is essential for RNA induced silencing complex (RISC) formation and it also forms a component of RISC loading complex (RLC) also known as the miRNA loading complex (miRLC) that contains - DICER1, EIF2C2/AGO2 and TARBP2. Within RLC/miRLC complex, DICER1 and TARBP2 are required to process precursor miRNAs to mature miRNAs and then load them onto EIF2C2/AGO2. Dicer also cleaves double-stranded RNA to produce short interfering RNAs (siRNAs) which targets selective destruction of complementary RNAs. siRNAs play a key roles in mitosis and DICER1 mutations have been shown to be associated with malignancies including blastoma, breast cancer, adenocarcinoma, and pancreatitis. It is also involved in myelination and other nervous system protections as well as pathologies including miRNA induced malformations of critical sensory cells in vision/hearing, cardiovascular diseases etc.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Slicing and Dicing RNA with Dicer Dicer is an RNaseIII-like enzyme capable of cleaving double-stranded RNA (dsRNA) into smaller 21-23 nt RNA fragments known as short interfering RNA (siRNAs). It targets the selective degradation of complementary RNAs in a posttranscriptional gene sile... Read full blog post.
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