Dicer Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: Dicer Antibody [NBP1-71691] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were ...read more
Immunohistochemistry-Paraffin: Dicer Antibody [NBP1-71691] - Analysis of Dicer in paraffin embedded human colorectal cancer tissue using anti-Dicer antibody. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: Dicer Antibody [NBP1-71691] - Dicer antibody was tested in HeLa cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry: Dicer Antibody [NBP1-71691] - Staining of Dicer in mouse prostate using DAB with hematoxylin counterstain.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Dicer Antibody Summary

Immunogen
A recombinant protein made to an internal region of the human Dicer protein (within residues 1100-1300). [Swiss-Prot# Q9UPY3]
Localization
Cytoplasm.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DICER1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2.0 ug/ml
  • Immunocytochemistry/Immunofluorescence 2-5 ug/ml
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
Application Notes
This Dicer antibody is useful for for Immunocytochemistry/Immunofluorescence and Immunohistochemistry-paraffin embedded sections. In ICC/IF, cytoplasmic staining was observed in HeLa cells. In IHC-P, staining was observed in the cytoplasm of mouse prostate. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Reviewed Applications
Read 1 Review rated 4
using
NBP1-71691 in the following applications:

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (86%) and Chinese Hamster (82%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for Dicer Antibody

  • DCR1
  • dicer 1, double-stranded RNA-specific endoribonuclease
  • dicer 1, ribonuclease type III
  • Dicer
  • Dicer1, Dcr-1 homolog (Drosophila)
  • EC 3.1.26
  • EC 3.1.26.-
  • EC 3.1.26.3
  • Helicase MOI
  • Helicase with RNase motif
  • helicase-moi
  • HERNADicer1, Dcr-1 homolog
  • K12H4.8-LIKE
  • KIAA0928endoribonuclease Dicer

Background

Dicer (DICER1 or double-strand-specific ribonuclease mDCR-1) belongs to RNase III family and is essential for RNA induced silencing complex (RISC) formation and it also forms a component of RISC loading complex (RLC) also known as the miRNA loading complex (miRLC) that contains - DICER1, EIF2C2/AGO2 and TARBP2. Within RLC/miRLC complex, DICER1 and TARBP2 are required to process precursor miRNAs to mature miRNAs and then load them onto EIF2C2/AGO2. Dicer also cleaves double-stranded RNA to produce short interfering RNAs (siRNAs) which targets selective destruction of complementary RNAs. siRNAs play a key roles in mitosis and DICER1 mutations have been shown to be associated with malignancies including blastoma, breast cancer, adenocarcinoma, and pancreatitis. It is also involved in myelination and other nervous system protections as well as pathologies including miRNA induced malformations of critical sensory cells in vision/hearing, cardiovascular diseases etc.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-03349
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-Fr, IP
NB100-56648
Species: Hu, Mu, Rt
Applications: WB
NBP2-24725
Species: Hu, Eq, Pm
Applications: WB, IHC, IHC-P
NB200-335
Species: Hu
Applications: WB, IHC, IHC-P, IP
NBP1-89606
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, KD
NBP1-59631
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-01770
Species: Hu, Mu, Rt, Ca, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-75619
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
AF6367
Species: Hu
Applications: WB, Flow, CyTOF-ready
AF3757
Species: Hu
Applications: WB, ICC
MAB1980
Species: Hu
Applications: WB, Simple Western, IP, ICC
NBP1-76985
Species: Hu, Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, Flow-CS
NB600-302
Species: Hu, Mu, Dr
Applications: WB, Simple Western, ChIP, ELISA, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
NBP1-30141
Species: Hu, Mu, Rt, Po, Ce, Dr, In, Ma, Ye
Applications: WB, ChIP, ELISA, ICC/IF, IHC, IHC-P, IP
NBP1-89327
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-71691
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P

Publications for Dicer Antibody (NBP1-71691) (0)

There are no publications for Dicer Antibody (NBP1-71691).
By submitting your publication information earn gift cards and discounts for future purchases.

Review for Dicer Antibody (NBP1-71691) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-71691:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin Dicer NBP1-71691
Enlarge
reviewed by:
Anonymous
IHC-P Human 05/08/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMultiple tissues
SpeciesHuman

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Dicer Antibody (NBP1-71691) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional Dicer Products

Array NBP1-71691

Bioinformatics Tool for Dicer Antibody (NBP1-71691)

Discover related pathways, diseases and genes to Dicer Antibody (NBP1-71691). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Dicer Antibody (NBP1-71691)

Discover more about diseases related to Dicer Antibody (NBP1-71691).
 

Pathways for Dicer Antibody (NBP1-71691)

View related products by pathway.

PTMs for Dicer Antibody (NBP1-71691)

Learn more about PTMs related to Dicer Antibody (NBP1-71691).
 

Research Areas for Dicer Antibody (NBP1-71691)

Find related products by research area.

Blogs on Dicer.

Slicing and Dicing RNA with Dicer
Dicer is an RNaseIII-like enzyme capable of cleaving double-stranded RNA (dsRNA) into smaller 21-23 nt RNA fragments known as short interfering RNA (siRNAs). It targets the selective degradation of complementary RNAs in a posttranscriptional gene sile...  Read full blog post.

Ago2 antibodies and dicer-independent biogenesis of miRNA
Ago2, also called eIF2C2, antibody is one of 37 reagents targeted to the Argonaute protein family that we at Novus Biologicals have in our antibody catalogue. Argonaute proteins are encoded by genes which play an important role in regulating the contr...  Read full blog post.

Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Anonymous
05/08/2015
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol DICER1
Entrez
Uniprot