Dicer Antibody (CL0378) - Azide and BSA Free

Staining of A-431 cells using the anti-Dicer monoclonal antibody) .

Staining of human breast cancer (ductal carcinoma) shows moderate cytoplasmic positivity in cancer cells.

Lane 1: Marker [kDa]Lane 2: Human cell line HEK293T

Staining of human colorectal cancer (adenocarcinoma) shows cytoplasmic positivity in cancer cells.

Staining of human cerebral cortex shows cytoplasmic positivity in the glial cells.

Staining of human prostate shows cytoplasmic immunoreactivity in the glandular epithelium cells.

Staining of human lung cancer (adenocacrinoma) shows strong cytoplasmic positivity in cancer cells.

Staining of human testis shows cytoplasmic positivity in the seminiferous tubules cells.

Staining of human uterus shows cytoplasmic immunoreactivity in the glandular epithelium cells.

Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-Dicer antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: CL0378
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Dicer Antibody (CL0378) - Azide and BSA Free Summary

• Immunogen: This antibody was generated using a recombinant protein sequence of Q9UPY3, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: DICER1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF Fixation Permeabilization: Use PFA/Triton X-100.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for Dicer Antibody (CL0378) - Azide and BSA Free

• DCR1
• dicer 1, double-stranded RNA-specific endoribonuclease
• dicer 1, ribonuclease type III
• Dicer
• Dicer1, Dcr-1 homolog (Drosophila)
• EC 3.1.26
• EC 3.1.26.-
• EC 3.1.26.3
• Helicase MOI
• Helicase with RNase motif
• helicase-moi
• HERNADicer1, Dcr-1 homolog
• K12H4.8-LIKE
• KIAA0928endoribonuclease Dicer

Background

Dicer encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DICER1