DHX36 Antibody - BSA Free

Western Blot: DHX36 Antibody [NBP2-92933] - Analysis of 200ug extracts of MCF7 cells using DHX36 at a dilition of 1:1000.

Western Blot: DHX36 Antibody [NBP2-92933] - Analysis of extracts of various cell lines, using DHX36 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

DHX36 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit DHX36 Antibody - BSA Free (NBP2-92933) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human DHX36 (NP_065916.2). MSYDYHQNWGRDGGPRSSGGGYGGGPAGGHGGNRGSGGGGGGGGGGRGGRGRHPGHLKGREIGMWYAKKQGQKNKEAERQERAVVHMDERREEQIVQLLNSVQAKNDKESEAQISWFAPEDHGYGTEVSTKNTPCSENKLDIQEKKLINQEKKMFRIRNRSYIDRDSEYLLQENEPDGTLDQKLLEDLQKKKNDLRYIEMQHFREKLPSYGMQKELVNLIDNHQVTVISGETGCGKTTQVTQFILDNYIERGKGSACRIV
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DHX36
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:50-1:100
  • Western Blot 1:200-1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for DHX36 Antibody - BSA Free

• DDX36
• DEAH (Asp-Glu-Ala-His) box polypeptide 36
• DEAH box protein 36
• EC 3.6.1
• EC 3.6.4.13
• G4 resolvase-1
• KIAA1488G4R1
• MLEL1RHAUDEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 36
• MLE-like protein 1
• probable ATP-dependent RNA helicase DHX36
• RNA helicase associated with AU-rich element ARE

Background

DHX36, also known as Probable ATP-dependent RNA helicase DHX36, consists of three isoforms of sizes 114.7 kDa, 113.2 kDa, and 111.5 kDa and is involved in breaking down mRNA with the ARE element in their 3'-UTR strand. Current research links this protein to sex development and spermatogenesis. The protein interacts with RPS9, AGO1, UBC, AGO2, and PHB in the RNA degradation pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DHX36