DDX3 Antibody (3B9E3)

Images

 
Western Blot: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis of DDX3X, DDX5 protein expression levels by Western blot in DTMUV-infected and control baby hamster kidney cell line BHK-21. From a verified customer review.
Immunohistochemistry: DDX3 Antibody (3B9E3) [NBP2-52457] - Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using DDX3X mouse mAb with DAB staining.
Flow Cytometry: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis of HepG2 cells using DDX3X mouse mAb (green) and negative control (red).
Western Blot: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis using DDX3X mouse mAb against Hela (1), NIH3T3 (2), C6 (3), COS7 (4), A431 (5), and HEK293 (6) cell lysate.
Western Blot: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis using DDX3X mAb against HEK293 (1) and DDX3X (AA: 518-661)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis using DDX3X mAb against human DDX3X (AA: 518-661) recombinant protein. (Expected MW is 41.4 kDa)
Immunohistochemistry: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis of paraffin-embedded cervical cancer tissues using DDX3X mouse mAb with DAB staining.
Flow Cytometry: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis of Hela cells using DDX3X mouse mAb (green) and negative control (red).
Immunofluorescence: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis of Hela cells using DDX3X mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Immunofluorescence: DDX3 Antibody (3B9E3) [NBP2-52457] - Immunofluorescence analysis of Hela cells using DDX3X mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ha, PmSpecies Glossary
Applications WB, ELISA, Flow, IHC, IHC-P, CyTOF-ready, ICC/IF
Clone
3B9E3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

DDX3 Antibody (3B9E3) Summary

Immunogen
Purified recombinant fragment of human DDX3X (AA: 518-661) expressed in E. Coli.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
DDX3X
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • CyTOF-ready
  • Immunocytochemistry 1:200-1:1000
  • Immunofluorescence
Application Notes
This antibody is Cytof ready. Western blot reported from a verified customer review.
Theoretical MW
73.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 4
using
NBP2-52457 in the following applications:

Publications
Read Publication using
NBP2-52457 in the following applications:

  • WB
    1 publication

Reactivity Notes

Hamster reactivity reported in scientific literature (PMID: 28516729).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for DDX3 Antibody (3B9E3)

  • DBXCAP-Rf
  • DDX14
  • DDX3DEAD/H box-3
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked
  • DEAD box protein 3, X-chromosomal
  • DEAD box, X isoform
  • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3
  • EC 3.6.1
  • EC 3.6.4.13
  • helicase like protein 2
  • Helicase-like protein 2
  • HLP2ATP-dependent RNA helicase DDX3X

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DDX3 Antibody (NBP2-52457)(1)

We have publications tested in 1 confirmed species: Hamster.

We have publications tested in 1 application: WB.


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WB
(1)
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Hamster
(1)
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Reviews for DDX3 Antibody (NBP2-52457) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, the baby hamster kidney cell line (BHK-21).

Reviews using NBP2-52457:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(1)
the baby hamster kidney cell line (BHK-21)
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot DDX3 NBP2-52457
Enlarge
3
reviewed by:
Anonymous
WB Human 06/21/2018
View

Summary

ApplicationWestern Blot
Sample Testedhela whole cell lysate
SpeciesHuman
Lot150828
Western Blot DDX3 NBP2-52457
Enlarge
5
reviewed by:
Anonymous
WB the baby hamster kidney cell line (BHK-21) 12/13/2017
View

Summary

ApplicationWestern Blot
Sample Testedthe baby hamster kidney cell line (BHK-21)
Speciesthe baby hamster kidney cell line (BHK-21)
LotNBP2-52457

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for DDX3 Antibody (NBP2-52457) (0)

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Secondary Antibodies

 

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Array NBP2-52457

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Recent Reviews

4
5
1
4
0
3
1
2
0
1
0

Anonymous
06/21/2018
Application: WB
Species: Human

Anonymous
12/13/2017
Application: WB
Species: the baby hamster kidney cell line (BHK-21)

Bioinformatics

Gene Symbol DDX3X