DHCR7 Antibody (HL3685)

Boiled and unboiled MCF-7 whole cell extract (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with DHCR7 antibody [HL3685] (NBP3-48834) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody

DHCR7 antibody [HL3685] detects DHCR7 protein by immunofluorescent analysis.Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.Green: DHCR7 stained by DHCR7 antibody [HL3685] (NBP3-48834) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .

Non-transfected (-) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with DHCR7 antibody [HL3685] (NBP3-48834) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: HL3685
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

DHCR7 Antibody (HL3685) Summary

• Description: Novus Biologicals Rabbit DHCR7 Antibody (HL3685) (NBP3-48834) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide of human DHCR7.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: DHCR7
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for DHCR7 Antibody (HL3685)

• 7-dehydrocholesterol reductase
• 7-DHC reductase
• D7SR
• delta-7-dehydrocholesterol reductase
• EC 1.3.1
• EC 1.3.1.21
• Putative sterol reductase SR-2
• SLOS
• Smith-Lemli-Opitz syndrome
• Sterol Delta(7)-reductase
• sterol delta-7-reductase

Background

The DHCR7 gene encodes delta-7-sterol reductase (EC 1.3.1.21), the penultimate enzyme of mammalian sterol biosynthesis that converts 7-dehydrocholesterol (7-DHC) to cholesterol. This enzyme removes the C(7-8) double bond introduced by the sterol delta8-delta7 isomerases. In addition, its role in drug-induced malformations is known: inhibitors of the last step of cholesterol biosynthesis such as AY9944 and BM15766 severely impair brain development (Moebius et al., 1998 [PubMed 9465114]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DHCR7