DGK-theta Antibody (652308)

Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, TF-1 human erythroleukemic cell line, HEK293 human embryonic kidney cell line, mouse brain tissue, and rat brain tissue. PVDF Membrane was probed with 1 µg/mL of Human DGK-theta Monoclonal Antibody (Catalog # MAB6598) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DGK-theta at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 652308
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

DGK-theta Antibody (652308) Summary

• Immunogen: E. coli-derived recombinant human DGK-theta
  Asp696-Lys839
  Accession # P52824
• Specificity: Detects DGK-theta in direct ELISAs and Western blots. In direct ELISAs and Western blolts, no cross-reactivity with recombinant human DGK-alpha , -beta , -gamma , -epsilon , -iota , -kappa , or -zeta is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: DGKQ
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DGK-theta Antibody (652308)

• DAG kinase theta
• DAGK
• DAGK4EC 2.7.1.107
• DAGK7
• DGKQ
• DGKtheta
• DGK-theta
• diacylglycerol kinase theta
• diacylglycerol kinase, theta (110kD)
• diacylglycerol kinase, theta 110kDa
• Diglyceride kinase theta

Background

Diacylglycerol kinase theta (DGK-theta ) is an approximately 110 kDa kinase that phosphorylates the lipid diacylglycerol. DGK-theta binds to RhoA, Akt, PKC epsilon and eta, and Steroidogenic Factor. It translocates to the nucleus or to the plasma membrane in response to NGF, TPA, or GPCR stimulation (thrombin, bradykinin, norepinephrine). DGK-theta contains three Cys-rich zinc finger regions (aa 60-108, aa 121-168, and aa 183-234), a Ras-associating domain (aa 395-494), two LxxLL motifs (aa 555-559 and aa 574-578), and a catalytic domain (aa 590-714). Over aa 696-839, human DGK-theta shares 90% aa sequence identity with mouse and rat DGK-theta .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DGKQ
• Uniprot:
  • P52824()