DGK-theta Antibody (652308) [PE]

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 652308
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: PE

DGK-theta Antibody (652308) [PE] Summary

• Immunogen: E. coli-derived recombinant human DGK-theta
  Asp696-Lys839
  Accession # P52824
• Specificity: Detects DGK-theta in direct ELISAs and Western blots. In direct ELISAs and Western blolts, no cross-reactivity with recombinant human DGK-alpha , -beta , -gamma , -epsilon , -iota , -kappa , or -zeta is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: DGKQ
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Alternate Names for DGK-theta Antibody (652308) [PE]

• DAG kinase theta
• DAGK
• DAGK4EC 2.7.1.107
• DAGK7
• DGKQ
• DGKtheta
• DGK-theta
• diacylglycerol kinase theta
• diacylglycerol kinase, theta (110kD)
• diacylglycerol kinase, theta 110kDa
• Diglyceride kinase theta

Background

Diacylglycerol (DAG) is a fundamental lipid second messenger that is produced in the nucleus. The accumulation of DAG in the nucleus is important for the regulation of cell growth and differentiation. Diacylglycerol kinases (DGKs) convert DAG to phosphatidic acid, thereby terminating diacylglycerol signaling, which results in the reduction of protein kinase C activity and cell cycle progression of T lymphocytes. Diacylglycerol kinases are divided into five subtypes, Type I-Type V. DGK-theta is a Type V DGK, and localizes mainly to the nucleus of various cell lines, such as MDA-MB-453, MCF-7, PC12 and HeLa. Nuclear DGK-theta co-localizes with phosphatidylinositol 4,5-bisphosphate (PIP(2)). DGK-theta is the isoform responsive to alpha-Thrombin stimulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DGKQ